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Polyspermy in Bufo arenarum oocytes matured in vitro

Published online by Cambridge University Press:  26 September 2008

J. Oterino ,
G. Sánchez Toranzo ,
L. Zelarayán  and
M.I. Bühler
J. Oterino
Affiliation:
Instituto Superior de Investigaciones Biológicas (INSIBIO), Departamento de Biología del Desarrollo, Universidad Nacional de Tucumán, Argentina
G. Sánchez Toranzo
Affiliation:
Instituto Superior de Investigaciones Biológicas (INSIBIO), Departamento de Biología del Desarrollo, Universidad Nacional de Tucumán, Argentina
L. Zelarayán
Affiliation:
Instituto Superior de Investigaciones Biológicas (INSIBIO), Departamento de Biología del Desarrollo, Universidad Nacional de Tucumán, Argentina
M.I. Bühler
Affiliation:
Instituto Superior de Investigaciones Biológicas (INSIBIO), Departamento de Biología del Desarrollo, Universidad Nacional de Tucumán, Argentina
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Summary

Full-grown ovarian oocytes of the amphibian Bufo arenarum were induced to mature in vitro by removing the follicular layers (spontaneous maturation) or by treatment with progesterone (hormone-induced maturation). These oocytes were then treated with trypsin and inseminated with homologous spermatozoa. Oocytes matured in vivo that had not undergone any influence of the oviducts (coelomic oocytes), inseminated under the same experimental conditions, were used as controls. The results show that oocytes induced to mature in vitro and exhibiting apparently normal signs of activation were polyspermic. In fact, 2 h after insemination numerous functioning pronuclei could be observed in the animal hemisphere. These results suggest that even though the oocytes which matured in vitro were able to undergo activation after insemination, they were unable to establish an effective block to polyspermy.

Keywords

AmphibianMaturation in vitroOvarian oocytesPolyspermy
Type
Article
Information
Zygote , Volume 5 , Issue 3 , August 1997 , pp. 267 - 271
Copyright
Copyright © Cambridge University Press 1997

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