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Phosphorylation of MAP kinase and p90rsk and its regulation during in vitro maturation of cumulus-enclosed rabbit oocytes

Published online by Cambridge University Press:  31 October 2002

Hai-Quan Yu ,
Shorgan Bou ,
Da-Yuen Chen  and
Qing-Yuan Sun
Hai-Quan Yu
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, P.R. China Key Laboratory for Mammalian Reproductive Biology and Biotechnology of Ministry of Education, Inner Mongolia University, Huhhot 010021, Inner Mongolia, P.R. China
Shorgan Bou
Affiliation:
Key Laboratory for Mammalian Reproductive Biology and Biotechnology of Ministry of Education, Inner Mongolia University, Huhhot 010021, Inner Mongolia, P.R. China
Da-Yuen Chen
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, P.R. China
Qing-Yuan Sun
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, P.R. China
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Abstract

Numerous studies have demonstrated that activation of the mitogen-activated protein (MAP) kinase is involved in the maturation of oocytes. In this study, the expression and phosphorylation of MAP kinase and p90rsk, one of the substrates of MAP kinase, during rabbit oocyte maturation were studied. The results showed that MAP kinase phosphorylation began to occur after germinal vesicle breakdown (GVBD) and the active form was maintained until metaphase II. p90rsk was also activated after GVBD following MAP kinase activation. Immunofluorescent analysis showed that p90rsk was enriched in the nuclear area after GVBD and was gradually localised to the spindle. When GVBD was inhibited by increased cAMP or decreased protein kinase C activity, the phosphorylation of both MAP kinase and p90rsk was blocked. Our data suggest that (1) MAP kinase/p90rsk activation is not necessary for GVBD, but plays an important role in the post-GVBD events including spindle assembly in rabbit oocytes; and (2) MAP kinase/p90rsk activation is down-regulated by cAMP and up-regulated by protein kinase C in cumulus-enclosed rabbit oocytes.

Keywords

MAP kinaseMicrotubuleOocytep90rskRabbit
Type
Research Article
Information
Zygote , Volume 10 , Issue 4 , November 2002 , pp. 311 - 316
Copyright
2002 Cambridge University Press

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