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Penetration of concanavalin-A-treated Chinese hamster oocytes by golden hamster spermatozoa in vitro, and chromosome analysis of hybrid 1-cell zygotes

Published online by Cambridge University Press:  26 September 2008

Seiji Watanabe ,
Hiroyuki Tateno  and
Yujiroh Kamiguchi
Seiji Watanabe*
Affiliation:
Department of Biological Sciences, Asahikawa Medical College, Asahikawa, Japan
Hiroyuki Tateno
Affiliation:
Department of Biological Sciences, Asahikawa Medical College, Asahikawa, Japan
Yujiroh Kamiguchi
Affiliation:
Department of Biological Sciences, Asahikawa Medical College, Asahikawa, Japan
*
Seiji Watanabe, Department of Biological Sciences, Asahikawa Medical College, 4–5 Nishikagura, Asahikawa 078, Japan. Telephone: +81-166-68-2731,. Fax: +81-166-65-5803
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Summary

Pretreatment of zona-free Chinese hamster (CH) oocytes with three kinds of lectin – concanavalin A (Con-A), phytohaemagglutinin-P (PHA) and wheat germ agglutinin (WGA) – was attempted in order to improve penetration by golden hamster (GH) spermatozoa in vitro. Con-A had no significant effect on penetration at 2 μg/ml, adequately facilitated oocyte-sperm fusion at 4 μg/ml, and caused excessive sperm binding and resultant severe polyspermy at 10 μg/ml. Neither PHA nor WGA had positive effects on sperm penetration at any concentrations (2–10 μg/ml) examined. Using the Con-A (4 μg/ml)pretreatment, high rates of interspecific fertilisation and subsequent chromosome analysis of hybrid 1-cell zygotes were achieved. Among 258 CH oocytes used, 212 (82.2%) were fertilised and 153 (72.2% of fertilised ova) developed to the first cleavage metaphase. Eventually, 132 CH-derived chromosome complements and 153 GH-derived ones were successfully karyoanalysed. Incidences of aneuploidy and structural anomaly were 3.1% and 2.3% in CH complements, and 1.4% and 6.5% in GH complements, respectively. These incidences were not significantly different from those obtained by intraspecific in vivo fertilisation, suggesting that our interspecific in vitro fertilisation system does not cause chromosome aberrations.

Keywords

Chinese hamster oocytesChromosomesGolden hamster spermatozoaInterspecific in vitro fertilisationLectin
Type
Article
Information
Zygote , Volume 4 , Issue 3 , August 1996 , pp. 167 - 172
Copyright
Copyright © Cambridge University Press 1996

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References

Refernces

Barnett, D.K. & Bavister, B.D.. (1992). Hypotaurine requirement for in vitro development of golden hamster one-cell embryos into morulae and blastocysts, and production of term offspring from in-vitro-fertilzed ova. Biol. Reprod. 47, 297–304.CrossRefGoogle ScholarPubMed
Bavister, B.D.. (1989). A consistently successful procedure for in vitro fertilization of golden hamster eggs. Gamete Res. 23, 139–58.CrossRefGoogle ScholarPubMed
Boerjan, M.L. & Boer, P.D.. (1990). First cell cycle of zygotes of the mouse derived from oocytes aged postovulation in vivo and fertilized in vivo. Mol. Reprod. Dev. 25, 155–63.CrossRefGoogle ScholarPubMed
Brandriff, B., Gordon, L., Ashworth, L., Watchmaker, G., Moore, D. II, Wyrobek, A.J. & Carrano, A.V.. (1985). Chromosomes of human sperm: variability among normal individuals Hum. Genet. 70, 18–24.CrossRefGoogle ScholarPubMed
Estop, A.M., Márquez, C., Munné, s., Navarro, J., Cieply, K., Kirk, V.V., Martorell, M.R., Benet, J. & Templado, C.. (1995). An analysis of human sperm chromosome breakpoints. Am. J. Hum. Genet. 56, 452–60.Google ScholarPubMed
Farrell, P.S. & Bavister, B.D.. (1984). Short-term exposure of two-cell hamster embryos to collection media is detrimental to viability. Biol. Reprod. 31, 109–14.CrossRefGoogle ScholarPubMed
Iizawa, Y., Kamiguchi, Y. & Mikamo, K.. (1992). A stable superovulation method in the Chinese hamster with two succesive administrations of PMSG. Jpn. J. Fertil Steril. 37, 445–50.Google Scholar
Juetten, J. & Bavister, B.D.. (1983). Effects of egg aging on in vitro fertilization and first cleavage division in the hamster. Gamete Res. 8, 219–30.CrossRefGoogle Scholar
Kamiguchi, Y., Tateno, H. & Mikamo, K.. (1994). Chromosomally abnormal gametes as a cause of developmental and congenital anomalies in humans. Cong. Anom. 34, 1–12.CrossRefGoogle Scholar
Kinsey, W.H. & Koehler, J.K.. (1976). Fine structural localization of concanavalin A binding sites on hamster spermatozoa. J. Supramol. Struct. 5, 185–98.CrossRefGoogle ScholarPubMed
Martin, R.H., Balkan, W., Burns, K., Rademaker, A.W., Lin, C.C. & Rudd, N.L.. (1983). The chromosome constitution of 1000 human spermatozoa. Hum. Genet. 63, 305–9.CrossRefGoogle ScholarPubMed
Mikamo, K. & Kamiguchi, Y.. (1983). A new assssment system for chromosomal mutagenicity using oocytes and early zygotes of the Chinese hamster. In: Radiation-Induced chromosome Damage in Man, ed. Ishihara, T & Sasaki, M.S., pp. 411–32. New York: Alan R. Liss.Google Scholar
Nicolson, G.L.Yanagimachi, R. & Yanagimachi, H.. (1975). Ultrastural localization of lectin-binding sites on the zonae pellucidae and plasma membranes of mammalian eggs. J. Cell Biol. 66, 263–74.CrossRefGoogle Scholar
Roldan, E.R.S. & Yanagimachi, R.. (1989). Cross-fertilization between Syrian and Chinese hamsters. J. Exp. Zool. 250. 321–8.CrossRefGoogle ScholarPubMed
Santaló, J., Estop, A.M. & Egozcue, J.. (1986). The chromosome complement of first-cleavage mouse embryos after in vitro fertilization. J. In Vitro Fertil. Embryo Transfer. 3, 99–105.CrossRefGoogle ScholarPubMed
Schini, S.A. & Bavister, B.D.. (1988). Two-cell block to development of cutured hamster embryos is caused by phosphate and glucose. Biol. Reprod. 39, 1183–92.CrossRefGoogle Scholar
Shaver, E.L. & Carr, D.H. (1967). Chromosome abnormalities in rabbit blastocysts following delayed fertilization. J. Reprod. Fertil. 14, 415–20.CrossRefGoogle ScholarPubMed
Tateno, H., Kamiguchi, Y., Shimada, M. & Mikamo, K.. (1996). Difference in types of radiation-induced structural chromosome aberrations and their incidences between Chinese and Syrian hamster spermatozoa. Mutat. Res. 350, 339–48.CrossRefGoogle ScholarPubMed
Vickers, A.D.. (1969). Delayed fertilization and chromosomal anomalies in mouse embroyos. J. Reprod. Fertil. 20, 69–76.CrossRefGoogle Scholar
Yanagimachi, R.. (1984). Zona-free hamster eggs: their use in assessing fertilizing capacity and examining chromosomes of human spermatozoa. Gamete res. 10, 187–232.CrossRefGoogle Scholar