Hostname: page-component-586b7cd67f-dsjbd Total loading time: 0 Render date: 2024-11-22T11:10:30.171Z Has data issue: false hasContentIssue false
  • English
  • Français

Nuclear-to-cytoplasmic ratios of 1PN and 2PN zygotes after in vitro fertilization of mouse oocytes

Published online by Cambridge University Press:  28 June 2021

Natsumi Okajima ,
Wei Xiao ,
Alex Lopata ,
Tadashi Sankai ,
Lubna Yasmin ,
Yasushi Nagai ,
Ryota Okamoto ,
Hidetaka Tasaki Open the ORCID record for Hidetaka Tasaki [Opens in a new window]  and
Junko Otsuki Open the ORCID record for Junko Otsuki [Opens in a new window]
Natsumi Okajima
Affiliation:
Department of Animal Science, Okayama University, Okayama, Japan
Wei Xiao
Affiliation:
Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan
Alex Lopata
Affiliation:
Retired, previously worked at the Department of Obstetrics and Gynecology, Royal Women’s Hospital, University of Melbourne, Australia
Tadashi Sankai
Affiliation:
Tsukuba Primate Research Center, National Institutes of Biomedical Innovation, Health and Nutrition, Ibaraki, Japan
Lubna Yasmin
Affiliation:
Department of Oncology, Cross Cancer Institute, University of Alberta, Alberta, Canada
Yasushi Nagai
Affiliation:
Nagai Mother’s Hospital, Saitama, Japan
Ryota Okamoto
Affiliation:
Department of Obstetrics and Gynecology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan
Hidetaka Tasaki
Affiliation:
Department of Animal Science, Okayama University, Okayama, Japan Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan Assisted Reproductive Technology Center, Okayama University, Okayama, Japan
Junko Otsuki*
Affiliation:
Department of Animal Science, Okayama University, Okayama, Japan Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan Assisted Reproductive Technology Center, Okayama University, Okayama, Japan
*
Author for correspondence: J. Otsuki. Department of Animal Science, Okayama University, Okayama, Japan. Email: [email protected]
Get access Rights & Permissions [Opens in a new window]

Summary

Numerous studies have reported comparisons of the nuclear-to-cytoplasmic (NC) ratio during mitosis. However, little information is known about how the pronuclear size is regulated and determined at the end of meiosis II in mammalian zygotes. The present study aims to analyze the NC ratio of female and male pronuclei, and also to compare the size of single pronuclei using photographs that were obtained during experiments to create chimeric hermaphrodites from 2-cell oocytes. The volume of both the female and the male pronucleus was found to correlate with the volume of the oocyte cytoplasm. The NC ratio of the male pronucleus was greater than that of the female pronucleus. The NC ratio of the average volume of the female and male pronuclei was greater than the NC ratio of the mononucleate oocytes. The occurrence of 1PN oocytes was significantly higher when the volume of cytoplasm was lower than the cut-off value. These results indicated that the NC ratio is retained during pronuclear formation. A higher NC ratio in male compared with the female pronucleus indicated structural and/or molecular difference between the two pronuclei. 1PN formation may occur when sperm enters close to the MII spindle.

Keywords

Pronucleuscell volumenucleus-cell ratiomeiosis1PN
Type
Research Article
Information
Zygote , Volume 30 , Issue 1 , February 2022 , pp. 120 - 124
Copyright
© The Author(s), 2021. Published by Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Adenot, PG, Mercier, Y, Renard, JP and Thompson, EM (1997). Differential H4 acetylation of paternal and maternal chromatin precedes DNA replication and differential transcriptional activity in pronuclei of 1-cell mouse embryos. Development 124, 4615–25.CrossRefGoogle ScholarPubMed
Aoki, F, Worrad, DM and Schultz, RM (1997). Regulation of transcriptional activity during the first and second cell cycles in the preimplantation mouse embryo. Dev Biol 181, 296–307.CrossRefGoogle ScholarPubMed
Balakier, H, Squire, J and Casper, RF (1993). Characterization of abnormal one pronuclear human oocytes by morphology, cytogenetics and in-situ hybridization. Hum Reprod 8, 402–8.CrossRefGoogle ScholarPubMed
Brownlee, C and Heald, R (2019). Importin α partitioning to the plasma membrane regulates intracellular scaling. Cell 176, 805–15.e8.CrossRefGoogle Scholar
Gurdon, JB (1976). Injected nuclei in frog oocytes: fate, enlargement, and chromatin dispersal. J Embryol Exp Morphol 36, 523–40.Google ScholarPubMed
Hertwig, R (1903). Ueber die Korrelation von Zell-und Kerngröße und ihre Bedeutung für die geschlechtliche Differenzierung und die Teilung der Zelle. Biol Centralblatt 23, 4–62.Google Scholar
Johnson, MH, Eager, D, Muggleton-Harris, A and Grave, HM (1975). Mosaicism in organisation concanavalin A receptors on surface membrane of mouse egg. Nature 257(5524), 321–2.CrossRefGoogle ScholarPubMed
Jorgensen, P, Edgington, NP, Schneider, BL, Rupes, I, Tyers, M and Futcher, B (2007). The size of the nucleus increases as yeast cells grow. Mol Biol Cell 18, 3523–32.CrossRefGoogle ScholarPubMed
Jovtchev, G, Schubert, V, Meister, A, Barow, M and Schubert, I (2006). Nuclear DNA content and nuclear and cell volume are positively correlated in angiosperms. Cytogenet Genome Res 114, 77–82.CrossRefGoogle ScholarPubMed
Levy, DL and Heald, R (2010). Nuclear size is regulated by importin α and Ntf2 in Xenopus . Cell 143, 288–98.CrossRefGoogle ScholarPubMed
Longo, FJ and Chen, DY (1984). Development of surface polarity in mouse eggs. Scan Electron Microsc (Pt 2), 703–16.Google Scholar
Neumann, FR and Nurse, P (2007). Nuclear size control in fission yeast. J Cell Biol 179, 593–600.CrossRefGoogle ScholarPubMed
Otsu, E, Sato, A, Nagaki, M, Araki, Y and Utsunomiya, T (2004). Developmental potential and chromosomal constitution of embryos derived from larger single pronuclei of human zygotes used in in vitro fertilization. Fertil Steril 81, 723–4.CrossRefGoogle ScholarPubMed
Otsuki, J, Iwasaki, T, Enatsu, N, Katada, Y, Furuhashi, K and Shiotani, M (2019). Noninvasive embryo selection: kinetic analysis of female and male pronuclear development to predict embryo quality and potential to produce live birth. Fertil Steril 112, 874–81.CrossRefGoogle ScholarPubMed
Otsuki, J, Iwasaki, T, Tsuji, Y, Katada, Y, Sato, H, Tsutsumi, Y, Hatano, K, Furuhashi, K, Matsumoto, Y, Kokeguchi, S and Shiotani, M (2017). Potential of zygotes to produce live births can be identified by the size of the male and female pronuclei just before their membranes break down. Reprod Med Biol 16, 200–5.CrossRefGoogle ScholarPubMed
Otsuki, J, Nagai, Y, Lopata, A, Chiba, K, Yasmin, L and Sankai, T (2012). Symmetrical division of mouse oocytes during meiotic maturation can lead to the development of twin embryos that amalgamate to form a chimeric hermaphrodite. Hum Reprod 27, 380–7.CrossRefGoogle Scholar
Santella, L, Alikani, M, Talansky, BE, Cohen, J and Dale, B (1992). Is the human oocyte plasma membrane polarized? Hum Reprod 7, 999–1003.CrossRefGoogle ScholarPubMed
Staessen, C, Janssenswillen, C, Devroey, P and Van Steirteghem, AC (1993). Cytogenetic and morphological observations of single pronucleated human oocytes after in-vitro fertilization. Hum Reprod 8, 221–3.CrossRefGoogle ScholarPubMed
Theerthagiri, G, Eisenhardt, N, Schwarz, H and Antonin, W (2010). The nucleoporin Nup188 controls passage of membrane proteins across the nuclear pore complex. J Cell Biol 189, 1129–42.CrossRefGoogle ScholarPubMed
Van Blerkom, J and Caltrider, K (2013). Sperm attachment and penetration competence in the human oocyte: a possible aetiology of fertilization failure involving the organization of oolemmal lipid raft microdomains influenced by the ΔΨm of subplasmalemmal mitochondria. Reprod Biomed Online 27, 690–701.CrossRefGoogle ScholarPubMed
Van Blerkom, J and Zimmermann, S (2016). Ganglioside-enriched microdomains define an oolemma that is functionally polarized with respect to fertilizability in the mouse. Reprod Biomed Online 33, 458–75.CrossRefGoogle ScholarPubMed
Worrad, DM, Ram, PT and Schultz, RM (1994). Regulation of gene expression in the mouse oocyte and early preimplantation embryo: developmental changes in Sp1 and TATA box-binding protein, TBP. Development 120, 2347–57.CrossRefGoogle ScholarPubMed