Hostname: page-component-cd9895bd7-jn8rn Total loading time: 0 Render date: 2024-12-24T00:55:03.687Z Has data issue: false hasContentIssue false
  • English
  • Français

Multiple aster formation is frequently observed in bovine oocytes retrieved from 1-day stored ovaries

Published online by Cambridge University Press:  03 March 2015

H. Hara ,
M. Tagiri ,
M. Hirabayashi  and
S. Hochi
H. Hara
Affiliation:
Interdisciplinary Graduate School of Science and Technology, Shinshu University, Ueda, Nagano 386–8567, Japan. National Institute for Physiological Sciences, Okazaki, Aichi 444–8787, Japan.
M. Tagiri
Affiliation:
Graduate School of Science and Technology, Shinshu University, Ueda, Nagano 386–8567, Japan.
M. Hirabayashi
Affiliation:
National Institute for Physiological Sciences, Okazaki, Aichi 444–8787, Japan.
S. Hochi*
Affiliation:
Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano 386–8567, Japan. Interdisciplinary Graduate School of Science and Technology, Shinshu University, Ueda, Nagano 386–8567, Japan. Graduate School of Science and Technology, Shinshu University, Ueda, Nagano 386–8567, Japan. Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano 386–8567, Japan.
*
All correspondence to: S. Hochi. Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano 386–8567, Japan. Tel: +81 268 215350. Fax: +81 268 215830. e-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Summary

We have recently reported that multiple aster formation after in vitro fertilization (IVF) was one of the factors that negatively affected the developmental competence of vitrified-warmed bovine matured oocytes, and that short-term culture of the post-warm oocytes with an inhibitor of Rho-associated coiled-coil kinase (ROCK) suppressed the multiple aster formation and improved the blastocyst yield. The present study was conducted to investigate whether increased multiple aster formation following IVF was involved in impaired developmental competence of stored ovary-derived bovine oocytes. Oocytes retrieved from 1-day stored ovaries had lower developmental potential to day 8 blastocysts when compared with those from fresh ovaries (37 versus 63%). Immunostaining of α-tubulin 10 h post-IVF revealed that a higher incidence of multiple aster formation occurred in oocytes retrieved from stored ovaries than from fresh ovaries (31 versus 15%). Treatment of post-in vitro maturated (post-IVM) oocytes with ROCK inhibitor for 2 h significantly suppressed the incidence of multiple aster formation (10 versus 32% in the control group). However, the suppression effect of ROCK inhibitor on multiple aster formation in IVM/IVF oocytes did not improve blastocyst yield from stored ovary-derived oocytes (41 versus 37% in the control group). These results suggested that the higher incidence of multiple aster formation by bovine ovary storage was not responsible for the decreased developmental competence of IVF oocytes.

Keywords

BlastocystsBovine IVFMultiple astersOvary storageROCK inhibitor (Y-27632)
Type
Research Article
Information
Zygote , Volume 24 , Issue 1 , February 2016 , pp. 115 - 120
Copyright
Copyright © Cambridge University Press 2015 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Hara, H., Abdalla, H., Morita, H., Kuwayama, M., Hirabayashi, M. & Hochi, S. (2011). Procedure for bovine ICSI, not sperm freeze-drying, impairs the function of the microtubule-organizing center. J. Reprod. Dev. 57, 428–32.Google Scholar
Hara, H., Hwang, I.S., Kagawa, N., Kuwayama, M., Hirabayashi, M. & Hochi, S. (2012). High incidence of multiple aster formation in vitrified-warmed bovine oocytes after in vitro fertilization. Theriogenology 77, 908–15.Google Scholar
Holm, P., Booth, P.J., Schmidt, M.H., Greve, T. & Callesen, H. (1999). High bovine blastocyst development in a static in vitro production system using SOFaa medium supplemented with sodium citrate and myo-inositol with or without serum-proteins. Theriogenology 52, 683–700.Google Scholar
Hwang, I.S., Hara, H., Chung, H.J., Hirabayashi, M. & Hochi, S. (2013). Rescue of vitrified-warmed bovine oocytes with Rho-associated coiled-coil kinase inhibitor. Biol. Reprod. 89, 26.Google Scholar
Iwata, H., Hayashi, T., Sato, H., Kimura, K., Kuwayama, T. & Monji, Y. (2005). Modification of ovary stock solution with magnesium and raffinose improves the developmental competence of oocytes after long preservation. Zygote 13, 303–8.CrossRefGoogle ScholarPubMed
Kim, N.H., Simerly, C., Funahashi, H., Schatten, G. & Day, B.N. (1996). Microtubule organization in porcine oocytes during fertilization and parthenogenesis. Biol. Reprod. 54, 1397–404.Google Scholar
Matsukawa, K., Akagi, S., Adachi, N., Kubo, M., Hirako, M., Watanabe, S. & Takahashi, S. (2007). Effect of ovary storage on development of bovine oocytes after intracytoplasmic sperm injection, parthenogenetic activation, or somatic cell nuclear transfer. J. Mamm. Ova Res. 24, 114–9.Google Scholar
Nagao, Y., Harada, Y., Yamaguchi, M., Igarashi, A., Ooshima, Y. & Kato, Y. (2010). Antioxidant treatment during preservation of bovine ovaries increased the development potential of embryos. Zygote 18, 315–21.Google Scholar
Nakao, H. & Nakatsuji, N. (1992). Effects of storage conditions of bovine ovaries and oocytes on the success rate of in vitro fertilization and culture. J. Reprod. Dev. 38, 11–3.CrossRefGoogle Scholar
Riento, K. & Ridley, A.J. (2003). ROCKs: multifunctional kinases in cell behaviour. Nat. Rev. Mol. Cell. Biol. 4, 446–56.Google Scholar
Rizos, D., Ward, F., Duffy, P., Boland, M.P. & Lonergan, P. (2002). Consequences of bovine oocyte maturation, fertilization or early embryo development in vitro versus in vivo: Implications for blastocyst yield and blastocyst quality. Mol. Reprod. Dev. 61, 234–48.CrossRefGoogle ScholarPubMed
Schatten, G. (1994). The centrosome and its mode of inheritance: The reduction of the centrosome during gametogenesis and its restoration during fertilization. Dev. Biol. 165, 299–335.CrossRefGoogle ScholarPubMed
Schofield, A.V., Steel, R. & Bernard, O. (2012). Rho-associated coiled-coil kinase (ROCK) protein controls microtubule dynamics in a novel signaling pathway that regulates cell migration. J. Biol. Chem. 287, 43620–9.Google Scholar
Schofield, A.V., Garmell, C., Suryadinata, R., Sarcevic, B. & Bernard, O. (2013). Tubulin polymerizing protein 1 (TPPP1) phosphorylation by Rho-associated coiled-coil kinase (ROCK) and cyclin-dependent kinase 1 (Cdk1) inhibits microtubule dynamics to increase cell proliferation. J. Biol. Chem. 288, 7907–17.CrossRefGoogle ScholarPubMed
Somfai, T., Imai, K., Kaneda, M., Akagi, S., Watanabe, S., Haraguchi, S., Mizutani, E., Dang-Nguyen, T.Q., Inaba, Y., Geshi, M. & Nagai, T. (2011). The effect of ovary storage and in vitro maturation on mRNA levels in bovine oocytes; A possible impact of maternal ATP1A1 on blastocyst development in slaughterhouse-derived oocytes. J. Reprod. Dev. 57, 723–30.Google Scholar
Sripunya, N., Somfai, T., Inaba, Y., Nagai, T., Imai, K. & Parnpai, R. (2010). A comparison of Cryotop and solid surface vitrification methods for the cryopreservation of in vitro matured bovine oocytes. J. Reprod. Dev. 56, 176–81.Google Scholar
Terada, Y., Nakamura, S., Simerly, C., Hewitson, L., Murakami, T., Yaegashi, N., Okamura, K. & Schatten, G. (2004). Centrosomal function assessment in human sperm using heterologous ICSI with rabbit eggs: A new male factor infertility assay. Mol. Reprod. Dev. 67, 360–5.Google Scholar