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Moderate heat treatment increases the penetrability of zonae pellucidae of salt-stored mammalian oocytes by spermatozoa

Published online by Cambridge University Press:  26 September 2008

Ryuzo Yanagimachi ,
Haruo Katayose ,
Gary Killian ,
Chin N. Lee ,
Douglas T. Carrell  and
Thomas T.F. Huang
Ryuzo Yanagimachi*
Affiliation:
Department of Anatomy and Reproductive Biology, University of Hawaii, Hawaii
Haruo Katayose
Affiliation:
Department of Anatomy and Reproductive Biology, University of Hawaii, Hawaii
Gary Killian
Affiliation:
Department of Anatomy and Reproductive Biology, University of Hawaii, Hawaii
Chin N. Lee
Affiliation:
Department of Anatomy and Reproductive Biology, University of Hawaii, Hawaii
Douglas T. Carrell
Affiliation:
Department of Anatomy and Reproductive Biology, University of Hawaii, Hawaii
Thomas T.F. Huang
Affiliation:
Department of Anatomy and Reproductive Biology, University of Hawaii, Hawaii
*
R. Yanagimachi, Department of Anatomy and Reproductive Biology, University of Hawaii School of Medicine, Honolulu, Hawaii 96822, USA. Tel: (808) 956-8746. Fax: (808) 956-5474.
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Summary

The zona pellucida of mammalian oocytes stored in highly concentrated solutions of neutral salts is known to retain its biological and biochemical properties. However, the zona may become resistant to sperm penetration as the storage period is increased. In cattle and hamsters, the penetrability of zonae of salt-stored oocytes was restored or increased by treating the oocytes with moderate heat without altering the gross morphology of the zona. Although this technique did not work for salt-stored human ova, this may have been due to the use of so-called inseminated- unfertilised ova which (1) may have been fertilised but failed to activate, or (2) were not fertilised because of functionally defective zonae.

Keywords

BovineHamsterHumanOocyteZona pellucida
Type
Article
Information
Zygote , Volume 1 , Issue 4 , November 1993 , pp. 345 - 351
Copyright
Copyright © Cambridge University Press 1993

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