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Micromorphological and ultrastructural description of spermatozoa from squirrel monkeys (Saimiri collinsi Osgood, 1916)

Published online by Cambridge University Press:  14 January 2020

Danuza L. Leão ,
Wlaisa V. Sampaio ,
Patrícia C. Sousa ,
Arlindo A.A. Moura Open the ORCID record for Arlindo A.A. Moura [Opens in a new window] ,
Irma C. Oskam ,
Regiane R. Santos Open the ORCID record for Regiane R. Santos [Opens in a new window]  and
Sheyla F.S. Domingues
Danuza L. Leão*
Affiliation:
Laboratory of Wild Animal Biotechnology and Medicine, Federal University of Pará, Belém, Pará, Brazil Post graduation programme in Animal Health and Production in the Amazon, Federal Rural University of the Amazon, Belém, Pará, Brazil
Wlaisa V. Sampaio
Affiliation:
Laboratory of Wild Animal Biotechnology and Medicine, Federal University of Pará, Belém, Pará, Brazil Post graduation programme in Animal Health and Production in the Amazon, Federal Rural University of the Amazon, Belém, Pará, Brazil Federal Rural University of the Amazon, Campus Parauapebas, Pará, Brazil
Patrícia C. Sousa
Affiliation:
Laboratory of Wild Animal Biotechnology and Medicine, Federal University of Pará, Belém, Pará, Brazil
Arlindo A.A. Moura
Affiliation:
Laboratory of Animal Physiology, Federal University of Ceará, Ceará,Brazil
Irma C. Oskam
Affiliation:
The Animal Production Experimental Center, Norwegian University of Life Sciences, Ås, Norway Faculty of Veterinary Medicine, Federal University of Pará, Castanhal, Pará, Brazil
Regiane R. Santos
Affiliation:
Laboratory of Wild Animal Biotechnology and Medicine, Federal University of Pará, Belém, Pará, Brazil
Sheyla F.S. Domingues
Affiliation:
Laboratory of Wild Animal Biotechnology and Medicine, Federal University of Pará, Belém, Pará, Brazil Post graduation programme in Animal Health and Production in the Amazon, Federal Rural University of the Amazon, Belém, Pará, Brazil The Animal Production Experimental Center, Norwegian University of Life Sciences, Ås, Norway Faculty of Veterinary Medicine, Federal University of Pará, Castanhal, Pará, Brazil
*
Author for correspondence: Danuza Leite Leão. Laboratory of Wild Animal Biotechnology and Medicine, Federal University of Pará, BR 316 Km 61, CEP 68740-970, Castanhal, Pará, Brazil. Tel: +55 91 33114707. E-mail: [email protected]
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Summary

Saimiri collinsi is used as an animal model in biotechnology research for conservation of species from the genus Saimiri. However, the development of biotechnologies depends on a proper knowledge of the sperm morphology to understand the basic aspects of sperm physiology, as potential male fertility depends on different cellular sperm structures. With this purpose, this study characterized the micromorphological and ultrastructural characteristics of squirrel monkeys (Saimiri collinsi) sperm using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). SEM electromyography revealed that a normal Saimiri collinsi sperm measures 71.7 ± 0.7 μm with lateral tail insertion, a paddle-shaped flattened head and an acrosome occupying most of the head. TEM also showed that the middle piece is characterized by a central 9 + 2 microtubule axoneme surrounded by nine dense fibres, and that the mitochondria were juxtaposed, forming the mitochondrial sheath. Here we provide the first micromorphological and ultrastructure description of S. collinsi sperm.

Keywords

Electron microscopyNeotropical primatesScanningSpermTransmission
Type
Research Article
Information
Zygote , Volume 28 , Issue 3 , June 2020 , pp. 203 - 207
Copyright
© Cambridge University Press 2020

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