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Lectin uptake and incorporation into the calcitic spicule of sea urchin embryos

Published online by Cambridge University Press:  15 April 2014

Nancy M. Mozingo
Nancy M. Mozingo*
Affiliation:
Biology Program, California State University, Channel Islands, Camarillo, California, USA
*
1 All correspondence to: Nancy M. Mozingo. Tel: +1 805 437 8989. Fax: +1 805 437 8895. e-mail: [email protected]
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Summary

Primary mesenchyme cells (PMCs) are skeletogenenic cells that produce a calcareous endoskeleton in developing sea urchin larvae. The PMCs fuse to form a cavity in which spicule matrix proteins and calcium are secreted forming the mineralized spicule. In this study, living sea urchin embryos were stained with fluorescently conjugated wheat germ agglutinin, a lectin that preferentially binds to PMCs, and the redistribution of this fluorescent tag was examined during sea urchin development. Initially, fluorescence was associated primarily with the surface of PMCs. Subsequently, the fluorescent label redistributed to intracellular vesicles in the PMCs. As the larval skeleton developed, intracellular granular staining diminished and fluorescence appeared in the spicules. Spicules that were cleaned to remove membranous material associated with the surface exhibited bright fluorescence, which indicated that fluorescently labelled lectin had been incorporated into the spicule matrix. The results provide evidence for a cellular pathway in which material is taken up at the cell surface, sequestered in intracellular vesicles and then incorporated into the developing spicule.

Keywords

BlastocoelCalciumPrimary mesenchyme cellsSkeletogenesisWheat germ agglutinin
Type
Short Communication
Information
Zygote , Volume 23 , Issue 3 , June 2015 , pp. 467 - 473
Copyright
Copyright © Cambridge University Press 2014 

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