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Isoloation of H-SF substances, the high-molecular-weight precursors of egg envelope preteins, from the ascites accumulated in the oestrogen-treated fish Oryzias latipes

Published online by Cambridge University Press:  26 September 2008

Kenji Murata ,
Ichiro Iuchi  and
Kenjiro Yamagami
Kenji Murata
Affiliation:
Life Science Institute, Sophia University, Tokyo, Japan.
Ichiro Iuchi
Affiliation:
Life Science Institute, Sophia University, Tokyo, Japan.
Kenjiro Yamagami*
Affiliation:
Life Science Institute, Sophia University, Tokyo, Japan.
*
Dr Kenjiro Yamagami, Life Science Institute, Sophia University, 7-1 Kioicho, Chiyoda-Ku, Tokyo 102, Japan. Telephone: (0)3-3238-3393. Fax: (0)3-3238-3393.
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Extract

High- and low-molecular weight spawning-female-specific (H-SF and L-SF) substances are liver-derived putative precursors of the inner layer of egg envelope of the medaka Oryzias latipes, and are also found in the ascites which are accumulated in the male fish on prolonged treatment with oestrogen. In the present study, the H-SF substances purified from the ascites of the oestrogen-treated male medaka were found to consist of three proteins. Their molecular weights ranged from 74 000 to 76 000, which corresponds to those of ZI-1 and ZI-2, the major components of medaka egg envelope. The amino acid composition of the H-SF substances was characterised by high contents of proline (17.58 mol%) and glutamic acid/glutamine (13.34 mol%). The characteristics were almost identical to those of ZI-1 and ZI-2 but differed from those of L-SF substance, a precursor of ZI-3, another major component of the egg envelope. These results confirm our view that H-SF substances are the precursors of ZI-1 and ZI-2. The average amino acid compositions of H-SF substances and L-SF subsatance coincided with the amino acid composition of whole egg envelope. This result strongly suggests that an equal amount of H-SF substances and L-SF substance would construct the inner layer of egg envelope after a little modification.

Keywords

Egg envelopeH-SF substancesOestrogenOogenesisOryzias latipes
Type
Article
Information
Zygote , Volume 1 , Issue 4 , November 1993 , pp. 315 - 324
Copyright
Copyright © Cambridge University Press 1993

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