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Isoloation of H-SF substances, the high-molecular-weight precursors of egg envelope preteins, from the ascites accumulated in the oestrogen-treated fish Oryzias latipes

Published online by Cambridge University Press:  26 September 2008

Kenji Murata ,
Ichiro Iuchi  and
Kenjiro Yamagami
Kenji Murata
Affiliation:
Life Science Institute, Sophia University, Tokyo, Japan.
Ichiro Iuchi
Affiliation:
Life Science Institute, Sophia University, Tokyo, Japan.
Kenjiro Yamagami*
Affiliation:
Life Science Institute, Sophia University, Tokyo, Japan.
*
Dr Kenjiro Yamagami, Life Science Institute, Sophia University, 7-1 Kioicho, Chiyoda-Ku, Tokyo 102, Japan. Telephone: (0)3-3238-3393. Fax: (0)3-3238-3393.
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Extract

High- and low-molecular weight spawning-female-specific (H-SF and L-SF) substances are liver-derived putative precursors of the inner layer of egg envelope of the medaka Oryzias latipes, and are also found in the ascites which are accumulated in the male fish on prolonged treatment with oestrogen. In the present study, the H-SF substances purified from the ascites of the oestrogen-treated male medaka were found to consist of three proteins. Their molecular weights ranged from 74 000 to 76 000, which corresponds to those of ZI-1 and ZI-2, the major components of medaka egg envelope. The amino acid composition of the H-SF substances was characterised by high contents of proline (17.58 mol%) and glutamic acid/glutamine (13.34 mol%). The characteristics were almost identical to those of ZI-1 and ZI-2 but differed from those of L-SF substance, a precursor of ZI-3, another major component of the egg envelope. These results confirm our view that H-SF substances are the precursors of ZI-1 and ZI-2. The average amino acid compositions of H-SF substances and L-SF subsatance coincided with the amino acid composition of whole egg envelope. This result strongly suggests that an equal amount of H-SF substances and L-SF substance would construct the inner layer of egg envelope after a little modification.

Keywords

Egg envelopeH-SF substancesOestrogenOogenesisOryzias latipes
Type
Article
Information
Zygote , Volume 1 , Issue 4 , November 1993 , pp. 315 - 324
Copyright
Copyright © Cambridge University Press 1993

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References

Hamazaki, T., Iuchi, I. & Yamagami, K. (1984). Chorion glycoprotein-like immunoreactivity in some tissues of adult female medaka. Zool Sci. 1, 148–50.Google Scholar
Hamazaki, T.S., Iuchi, I. & Yamagami, K. (1985). A spawning female-specific substance reactive to anti-chorion (egg envelope) glycoprotein antibody in the teleost. Oryzias latipes. J. Exp. Zool. 235, 269–79.CrossRefGoogle Scholar
Hamazaki, T.S., Iuchi, I. & Yamagami, K. (1987)a. production of a ‘spawning female-specific substance’ in hepatic cells and its accumulation in the ascites of the estrogen-treated adult fish, Oryzias latipes. J. Exp. Zool. 242, 325–32.CrossRefGoogle Scholar
Hamazaki, T.S., Iuchi, I. & Yamagami, K. (1986b). Purification and identification of vitellogenin and its immunohistochemical detection in growing oocytes of the teleost, Oryzias latipes. J. Exp. Zool. 242, 333–41.CrossRefGoogle Scholar
Hamazaki, T.S., Iuchi, I. & Yamagami, K. (1987c). Isolation and partial characterization of a ‘spawning female-specific substance’ in the teleost, Oryzias latipes. J.Exp Zool. 242, 343–9.CrossRefGoogle Scholar
Hamazaki, T.S.,Nagahama, Y., Iuchi, I. & Yamagami, K. (1989). A glycoprotein from the liver constitutes the inner layer of the egg envelope (zona pellucida interna) of the fish, Oryzias latipes Dev. Biol. 133,101–10.CrossRefGoogle ScholarPubMed
Hyllner, S.J. & Haux, C. (1992). Immunochemical detection of the major vitelline envelope proteins in the plasma and oocytes of the maturing female rainbow trout, Oncorhynchus mykiss. J. Endocrinol. 135, 303–9.CrossRefGoogle ScholarPubMed
Hyllner, S.J., Oppen-Berntsen, D.O., Helvik, J.V., Walther, B.T. & Haux, C. (1991). Oestradiol-17βinduces the major vitelline envelope proteins in both sexes in teleosts. J. Endocrinol. 131, 229–36.CrossRefGoogle ScholarPubMed
Iuchi, I., Yamagami, K. (1976). Major glycoproteins solubilised from the teleostean egg membrane by the action of the hatching enzyme Biochim. Biophys. Acta 453, 240–9.CrossRefGoogle ScholarPubMed
Khyse-Anderson, J. (1984). Electroblotting of multiple gels: a single apparatus without buffer tank for rapid transfer of protein from polyacrylamide to nitrocellulose. J. Biochem. Biophys. Methods 10, 203–9.CrossRefGoogle Scholar
Laemmli, U.K. (1970). Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature 227, 680–5.CrossRefGoogle ScholarPubMed
Murata, K., Hamazaki, T.S., Iuchi, I. & Yamagami, K. (1991). Spawning female-specific egg envelope glycoprotein-like substances in Oryzias latipes. Dev. Growth Differ. 33, 553–62.Google Scholar
Oppen-Berntsen, D.O., Gram-Jansen, E. & Walther, B.T. (1992a). Zona radiata proteins are synthesized by rainbow trout (Oncorhycus mykiss) hepatocytes in response to oestradiol-17β. Endocrinol. 135, 293–302.Google Scholar
Oppen-Berntsen, D.O., Hyllner, S.J., Haux, C., Helvik, J.V. & Walther, B.T. (1992b). Eggshell zona radiata proteins from cod (Gadus morhua): extra-ovarian origin and induction by estradiol-;17β. Int. J. Dev. Biol. 36, 247–54.Google Scholar
Ornstein, L. & Davis, B.J. (1962). Disc Electrophoresis. Reprinted by Distillation Products Industries, Eastman Kodak Co.Google Scholar
Tesoriero, J.V. (1976). Formation of the chorion (zona pellucida) in the teleost, Oryzias latipes: autoradiography of [3H] proline incorporation. J. Ultrastruct. Res. 64, 315–26.CrossRefGoogle Scholar
Towbin, H. (1979). Electrophoretic transfer of protein from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 79, 4350–4.CrossRefGoogle Scholar
Waldeyer, W. (1906). In: Handbuch der vergleichenden und experimentellen Entwicklungslehere, ed. Hertwig, O., pp. 86–476.: Jena: Fischer.Google Scholar
Wilson, E.B. (1928). The cell in Development and Heredity, 3rd edn.London: MacMillan.Google Scholar
Yamagami, K. (1981). Mechanisms of hatching in fish: secretion of hatching enzyme and enzymatic choriolysis. Am. Zool. 21, 459–71.CrossRefGoogle Scholar
Yamagami, K., Hamazaki, T.S., Yasumasu, S., Masuda, K. & Iuchi, I. (1992). Molecular and cellular basis of formation, hardening, and breakdown of the egg envelope in fish. Int. Rev. Cytol. 136, 51–92.Google Scholar