Hostname: page-component-586b7cd67f-t8hqh Total loading time: 0 Render date: 2024-11-23T00:49:08.208Z Has data issue: false hasContentIssue false
  • English
  • Français

Involvement of PLA2, COX and LOX in Rhinella arenarum oocyte maturation

Published online by Cambridge University Press:  27 February 2013

Maria Eugenia Ortiz ,
Marta Inés Bühler  and
Liliana Isabel Zelarayán
Maria Eugenia Ortiz
Affiliation:
Centro de Referencias para Lactobacilos (CERELA)-CONICET, Chacabuco 145, Tucumán, Argentina.
Marta Inés Bühler
Affiliation:
Instituto de Biología, Facultad de Bioqca, Qca. y Farmacia, INSIBIO-UNT, Chacabuco 461, Tucumán, Argentina.
Liliana Isabel Zelarayán*
Affiliation:
Instituto de Biología, Facultad de Bioqca, Qca. y Farmacia, INSIBIO-UNT, Chacabuco 461, 4000. San Miguel de Tucumán, Argentina.
*
All correspondence to: Liliana Isabel Zelarayán. Instituto de Biología, Facultad de Bioqca, Qca. y Farmacia, INSIBIO-UNT, Chacabuco 461, 4000. San Miguel de Tucumán, Argentina. Tel: +54 381 4247752 Ext. 7093. Fax: +54 381 4247752 Ext. 7004. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Summary

In Rhinella arenarum, progesterone is the physiological nuclear maturation inducer that interacts with the oocyte surface and starts a cascade of events that leads to germinal vesicle breakdown (GVBD). Polyunsaturated fatty acids and their metabolites produced through cyclooxygenase (COX) and lipoxygenase (LOX) pathways play an important role in reproductive processes. In amphibians, to date, the role of arachidonic acid (AA) metabolites in progesterone (P4)-induced oocyte maturation has not been clarified. In this work we studied the participation of three enzymes involved in AA metabolism – phospholipase A2 (PLA2), COX and LOX in Rhinella arenarum oocyte maturation. PLA2 activation induced maturation in Rhinella arenarum oocytes in a dose-dependent manner. Oocytes when treated with 0.08 μM melittin showed the highest response (78 ± 6% GVBD). In follicles, PLA2 activation did not significantly induce maturation at the assayed doses (12 ± 3% GVBD). PLA2 inhibition with quinacrine prevented melittin-induced GVBD in a dose-dependent manner, however PLA2 inactivation did not affect P4-induced maturation. This finding suggests that PLA2 is not the only phospholipase involved in P4-induced maturation in this species. P4-induced oocyte maturation was inhibited by the COX inhibitors indomethacin and rofecoxib (65 ± 3% and 63 ± 3% GVBD, respectively), although COX activity was never blocked by their addition. Follicles showed a similar response following the addition of these inhibitors. Participation of LOX metabolites in maturation seems to be correlated with seasonal variation in ovarian response to P4. During the February to June period (low P4 response), LOX inhibition by nordihydroguaiaretic acid or lysine clonixinate increased maturation by up to 70%. In contrast, during the July to January period (high P4 response), LOX inhibition had no effect on hormone-induced maturation.

Keywords

CyclooxygenaseLipoxygenaseOocyte maturationPhospholipase A2Rhinella arenarum
Type
Research Article
Information
Zygote , Volume 22 , Issue 4 , November 2014 , pp. 440 - 445
Copyright
Copyright © Cambridge University Press 2013 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Caldironi, H.A. & Alonso, T.S. (1996). Lipid characterization of full-grown amphibian oocytes and their plasma membrane-enriched fractions. Lipids 31, 651–6.Google Scholar
Cerdá, J., Zanuy, S., Carrillo, M., Ramos, J. & Serrano, R. (1995). Short- and long-term dietary effects on female sea bass (Dicentrarchus labrax): seasonal changes in plasma profiles of lipids and sex steroids in relation to reproduction. Comp. Biochem. Physiol. Part C: Pharmacology, Toxicology and Endocrinology 111, 83–91.Google Scholar
Cerdá, J., Zanuy, S. & Carrillo, M. (1997). Evidence for dietary effects on plasma levels of sexual steroids during spermatogenesis in the sea bass. Aquacult. Int. 5, 473–7.Google Scholar
Diouf, M.N., Sayasith, K., Lefebvre, R., Silversides, D.W., Sirois, J. & Lussier, J.G. (2006). Expression of phospholipase A2 group IVA (PLA2G4A) is upregulated by human chorionic gonadotropin in bovine granulose cells of ovulatory follicles. Biol. Reprod. 74, 1096–103.Google Scholar
Johnson, A.L., Tilly, J.L. & Levorse, J.M. (1991). Possible role for arachidonic acid in the control of steroidogenesis in hen theca. Biol. Reprod. 44, 338–44.Google Scholar
Kurusu, S., Iwao, M., Kawaminami, M. & Hashimoto, I. (1998). Involvement of cytosolic phospholipase A2 in the ovulatory process in gonadotropin-primed immature rats. Prostaglandins Leukot. Essent. Fatty Acids 58, 405–11.CrossRefGoogle ScholarPubMed
Lin, T. (1985). Mechanism of action of gonadotropin-releasing hormone stimulated Leydig cell steroidogenesis III. The role of arachidonic acid and calcium/phospholipid dependent protein kinase. Life Sci. 36, 1255–64.Google Scholar
Lister, A.L. & Van Der Kraak, G. (2008). An investigation into the role of prostaglandins in zebrafish oocyte maturation and ovulation. Gen. Comp. Endocrinol. 159, 46–57.Google Scholar
Mata, A., Zelarayán, L.I., Sánchez Toranzo, G., Alonso, T. & Bühler, M.I. (2000). Neutral lipid characterization of Bufo arenarum oocyte capable and not capable of undergoing spontaneous maturation. Abstract of XVI Annual Scientific Meeting, Tucuman Biology Society. Biocell 24, 134.Google Scholar
Medina, M., Ramos, I., Crespo, C.A., González-Calvar, S. & Fernández, S.N. (2004). Changes in serum sex steroid levels throughout the reproductive cycle of Bufo arenarum females. Gen. Comp. Endocrinol. 136, 143–51.Google Scholar
Meijer, L., Brash, A., Bryant, R., Kwokei, N.G., Maclouff, J. & Sprecher, H. (1986). Stereospecific induction of starfish oocyte maturation by (8R)-hydroxyeicosatetraenoic acid. J. Biol. Chem. 261, 17040–7.CrossRefGoogle ScholarPubMed
Navas, J.M., Bruce, M., Thrush, M., Farndale, B.N., Bromage, N., Zanuy, S., Carrillo, M., Bell, J. & Ramos, J. (1997). The impact of seasonal alteration in the lipid composition of broodstock diets on egg quality in the European of sea bass (Dicentrarchus labrax). J. Fish Biol. 51, 760–73.Google Scholar
Navas, J.M., Mañanos, E., Thrush, M., Ramos, J., Zanuy, S., Carrillo, M., Zohar, Y. & Bromage, N. (1998). Effect of dietary lipid composition on vitellogenin, 17β-estradiol and gonadotropin plasma levels and spawning performance in captive sea bass (Dicentrarchus labrax). Aquaculture 165, 65–79.Google Scholar
Patiño, R., Yoshizaki, G., Bolamba, D. & Thomas, P. (2003). Role of arachidonic acid and protein kinase C during maturation-inducing hormone-dependent meiotic resumption and ovulation in ovarian follicles of Atlantic croaker. Biol. Reprod. 68, 516–23.Google Scholar
Sánchez Toranzo, G., Bonilla, F., Zelarayán, L., Oterino, J. & Bühler, M.I. (2006). Activation of maturation promoting factor in Bufo arenarum oocytes: injection of mature cytoplasm and germinal vesicle contents. Zygote 14, 305–16.Google Scholar
Sorbera, L.A., Asturiano, J.F., Carrillo, M. & Zanuy, S. (2001). Effects of polyunsaturated fatty acids and prostaglandins on oocyte maturation in a marine teleost, the European Sea Bass (Dicentrarchus labrax). Biol. Reprod. 64, 382–9.Google Scholar
Zelarayán, L.I., Alonso, T., Mata, A., Sánchez Toranzo, G., Oterino, J. & Bühler, M.I. (1999). Evaluation of parameters relationed with the Bufo arenarum oocyte competence to mature spontaneously. Abstract of XV Annual Scientific Meeting, Tucuman Biology Society. Biocell 23, 84.Google Scholar
Zelarayán, L.I., Sánchez Toranzo, G., Oterino, J. & Buhler, M. (2000). Involvement of purines and phosphoinositides on spontaneous and progesterone induced maturation in Bufo arenarum oocytes. J. Exp. Zool. 287, 151–7.Google Scholar
Zelarayán, L.I., Ortiz, M.E., Unáas, L., Ajmat, M.T., Bonilla, F., Sánchez Toranzo, G. & Bühler, M.I. (2009). Prostaglandins effects on Rhinella arenarum oocyte maturation. Abstract of XXV Annual Scientific Meeting, Tucuman Biology Association. Biocell 33, 161.Google Scholar
Zelarayán, LI., Ajmat, M.T., Bonilla, F. & Bühler, M.I. (2012). Involvement of G protein and purines in Rhinella arenarum oocyte maturation. Zygote 2, 1–10.Google Scholar