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Interference of fixatives and fixation period on the morphologic analysis of ovarian preantral follicles

Published online by Cambridge University Press:  14 May 2021

D.C.C. Brito ,
L.V.S. Ñaupas ,
S.S. Souza ,
G.L.H. Alcântara ,
J.R. Figueiredo ,
R.R. Santos Open the ORCID record for R.R. Santos [Opens in a new window]  and
A.P.R. Rodrigues
D.C.C. Brito
Affiliation:
Laboratory of Manipulation of Oocytes and Ovarian Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, Ceará State University, Fortaleza, CE, Brazil
L.V.S. Ñaupas
Affiliation:
Laboratory of Manipulation of Oocytes and Ovarian Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, Ceará State University, Fortaleza, CE, Brazil
S.S. Souza
Affiliation:
Laboratory of Manipulation of Oocytes and Ovarian Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, Ceará State University, Fortaleza, CE, Brazil
G.L.H. Alcântara
Affiliation:
Laboratory of Manipulation of Oocytes and Ovarian Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, Ceará State University, Fortaleza, CE, Brazil
J.R. Figueiredo
Affiliation:
Laboratory of Manipulation of Oocytes and Ovarian Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, Ceará State University, Fortaleza, CE, Brazil
R.R. Santos*
Affiliation:
Schothorst Feed Research, Lelystad, The Netherlands
A.P.R. Rodrigues
Affiliation:
Laboratory of Manipulation of Oocytes and Ovarian Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, Ceará State University, Fortaleza, CE, Brazil
*
Author for correspondence: R.R. Santos. Schothorst Feed Research, Lelystad, The Netherlands. E-mail: [email protected]
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Summary

Ovine ovarian fragments (3 × 3 × 1 mm) were fixed in neutral buffered formalin (NBF), Carnoy’s solution (CAR), Davidson’s solution (DAV), or paraformaldehyde (PFA) for 12 h or 24 h. After this fixation time, each fragment was prepared for histological analysis. Although fixative and fixation period did not affect follicular and stromal cells density, the percentages of morphologically normal primordial and primary follicles was affected by the fixative type and period of fixation. Paraformaldehyde was not indicated as a fixative for ovarian fragments. Formalin was a suitable fixative only when the period of fixation was 12 h, while Carnoy was efficient after a fixation period of 24 h. In conclusion, the most indicated fixative for the morphological evaluation of ovarian preantral follicles was DAV, regardless of the fixation period, that is 12 or 24 h.

Keywords

AssessmentPrimary folliclesPrimordial folliclesStromal cells histology
Type
Short Communication
Information
Zygote , Volume 30 , Issue 1 , February 2022 , pp. 144 - 147
Copyright
© The Author(s), 2021. Published by Cambridge University Press

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