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Inhibition of boar sperm hyaluronidase activity by tannic acid reduces polyspermy during in vitro fertilization of porcine oocytes

Published online by Cambridge University Press:  01 November 2006

Hideki Tatemoto ,
Isao Tokeshi ,
Satoshi Nakamura ,
Norio Muto  and
Tadashi Nakada
Hideki Tatemoto*
Affiliation:
Department of Bioproduction, Faculty of Agriculture, University of the Ryukyus, Nishihara-cho, Okinawa 903-0213, Japan.
Isao Tokeshi
Affiliation:
Department of Bioproduction, Faculty of Agriculture, University of the Ryukyus, Nishihara-cho, Okinawa 903-0213, Japan.
Satoshi Nakamura
Affiliation:
Laboratory of Swine, Okinawa Prefectural Livestock Experiment Station, Nakijin-son, Okinawa 905-0426, Japan.
Norio Muto
Affiliation:
Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima, Shobara, Hiroshima 727-0023, Japan.
Tadashi Nakada
Affiliation:
Department of Bioproduction, Faculty of Agriculture, University of the Ryukyus, Nishihara-cho, Okinawa 903-0213, Japan.
*
All correspondence to: H. Tatemoto, Department of Bioproduction, Faculty of Agriculture, University of the Ryukyus, Nishihara-cho, Okinawa 903-0213, Japan. Fax: +81 98 895 8757. e-mail: [email protected]
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Summary

The present study was conducted to examine the effects of three polyphenols (tannic acid, apigenin and quercetin) on hyaluronidase activity and in vitro fertilization (IVF) parameters. Among them, tannic acid showed by far the strongest potency for blocking hyaluronidase activity extracted from preincubated boar sperm, causing a dose-dependent inhibition over the range of 2–10 μg/ml. When cumulus-intact and cumulus-free oocytes were inseminated in IVF medium containing tannic acid, the penetration and the polyspermy rates were significantly decreased in the presence of 10 μg/ml tannic acid compared with those in the absence of tannic acid, and the addition of 5 μg/ml tannic acid significantly reduced the polyspermy rate (p < 0.05) compared with that of the control while maintaining the high penetration rate. However, apigenin and quercetin had no effect on the rate of polyspermy. Interestingly, the incidence of polyspermy was significantly reduced in oocytes inseminated with sperm pretreated with 5 μg/ml tannic acid (p < 0.05), although the pretreatment of oocytes had no effect against the polyspermy after insemination with untreated sperm. Treatment with tannic acid caused neither a protective proteolytic modification of the zona pellucida matrix before fertilization, nor a reduction of the proteolytic activity of acrosomal contents or the number of zona-bound spermatozoa. These data suggest that an appropriate concentration of tannic acid prevents polyspermy through the inhibition of sperm hyaluronidase activity during IVF of porcine oocytes.

Keywords

Boar spermatozoaHyaluronidaseIn vitro fertilizationPolyphenolsPolyspermy
Type
Research Article
Information
Zygote , Volume 14 , Issue 4 , November 2006 , pp. 275 - 285
Copyright
Copyright © Cambridge University Press 2006

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