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In vitro maturation alters gene expression in bovine oocytes

Published online by Cambridge University Press:  17 February 2016

Paulo R. Adona ,
Cláudia L.V. Leal ,
Fernando H. Biase ,
Tiago H. De Bem ,
Lígia G. Mesquita ,
Flávio V. Meirelles ,
André L. Ferraz ,
Luiz R. Furlan ,
Paulo S. Monzani  and
Samuel Guemra
Paulo R. Adona*
Affiliation:
Agropecuária Laffranchi. PO box 45. Zip Code: 86125-000 – Tamarana, Paraná. Brazil. Escola de Medicina Veterinária, Universidade Norte do Paraná, Arapongas, PR, Brazil. Departamento de Ciências Básicas, Universidade de São Paulo, Pirassununga, SP, Brazil.
Cláudia L.V. Leal
Affiliation:
Departamento de Ciências Básicas, Universidade de São Paulo, Pirassununga, SP, Brazil.
Fernando H. Biase
Affiliation:
Department of Animal Sciences, Auburn University, Auburn, AL, USA.
Tiago H. De Bem
Affiliation:
Departamento de Ciências Básicas, Universidade de São Paulo, Pirassununga, SP, Brazil.
Lígia G. Mesquita
Affiliation:
Departamento de Ciências Básicas, Universidade de São Paulo, Pirassununga, SP, Brazil.
Flávio V. Meirelles
Affiliation:
Departamento de Ciências Básicas, Universidade de São Paulo, Pirassununga, SP, Brazil.
André L. Ferraz
Affiliation:
Escola de Zootecnia, Universidade estadual de Mato Grosso do Sul, Aquidauana, MS, Brazil.
Luiz R. Furlan
Affiliation:
Departamento de Tecnologia, Universidade Estadual Paulista, Botucatu, SP, Brazil.
Paulo S. Monzani
Affiliation:
Escola de Medicina Veterinária, Universidade Norte do Paraná, Arapongas, PR, Brazil. Laboratório de Reprodução Animal, Agropecuária Laffranchi, Tamarana, PR, Brazil.
Samuel Guemra
Affiliation:
Escola de Medicina Veterinária, Universidade Norte do Paraná, Arapongas, PR, Brazil. Laboratório de Reprodução Animal, Agropecuária Laffranchi, Tamarana, PR, Brazil.
*
All correspondence to: Paulo R. Adona. Agropecuária Laffranchi. PO box 45. Zip Code: 86125-000 – Tamarana, Paraná. Brazil. Tel: +55 43 33994700. E-mail: [email protected]
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Summary

Gene expression profiling of in vivo- and in vitro-matured bovine oocytes can identify transcripts related to the developmental potential of oocytes. Nonetheless, the effects of in vitro culturing oocytes are yet to be fully understood. We tested the effects of in vitro maturation on the transcript profile of oocytes collected from Bos taurus indicus cows. We quantified the expression of 1488 genes in in vivo- and in vitro-matured oocytes. Of these, 51 genes were up-regulated, whereas 56 were down-regulated (≥2-fold) in in vivo-matured oocytes in comparison with in vitro-matured oocytes. Quantitative real-time polymerase chain reaction (PCR) of nine genes confirmed the microarray results of differential expression between in vivo- and in vitro-matured oocytes (EZR, EPN1, PSEN2, FST, IGFBP3, RBBP4, STAT3, FDPS and IRS1). We interrogated the results for enrichment of Gene Ontology categories and overlap with protein–protein interactions. The results revealed that the genes altered by in vitro maturation are mostly related to the regulation of oocyte metabolism. Additionally, analysis of protein–protein interactions uncovered two regulatory networks affected by the in vitro culture system. We propose that the differentially expressed genes are candidates for biomarkers of oocyte competence. In vitro oocyte maturation can affect the abundance of specific transcripts and are likely to deplete the developmental competence.

Type
Research Article
Information
Zygote , Volume 24 , Issue 4 , August 2016 , pp. 624 - 633
Copyright
Copyright © Cambridge University Press 2016 

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