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In vitro development of nuclear transfer embryos derived from porcine embryonic germ cells and their descendent neural precursor cells

Published online by Cambridge University Press:  12 July 2011

Susa Shin ,
Kwang Sung Ahn ,
Seong-Jun Choi ,
Soon Young Heo  and
Hosup Shim
Susa Shin
Affiliation:
Department of Physiology, Dankook University School of Medicine, Cheonan, Korea.
Kwang Sung Ahn
Affiliation:
Department of Physiology, Dankook University School of Medicine, Cheonan, Korea.
Seong-Jun Choi
Affiliation:
StemK Inc., Ansan, Korea.
Soon Young Heo
Affiliation:
Department of Nanobiomedical Science and WCU Research Center for Nanobiomedical Science, Dankook University, Cheonan, Korea.
Hosup Shim*
Affiliation:
Department of Nanobiomedical Science and WCU Center for Nanobiomedical Science, Dankook University, San 29 Anseo-dong, Dongnam-gu, Cheonan, Chungnam 330–714, Korea. Institute of Tissue Regeneration Engineering, Dankook University, Cheonan, Korea.
*
All correspondence to: Hosup Shim. Department of Nanobiomedical Science and WCU Center for Nanobiomedical Science, Dankook University, San 29 Anseo-dong, Dongnam-gu, Cheonan, Chungnam 330–714, Korea. Tel: +82 41 550 3865. Fax: +82 41 550 1149. e-mail: [email protected]
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Summary

Undifferentiated stem cells may support a greater development of cloned embryos compared with differentiated cell types due to their ease of reprogramming during the nuclear transfer (NT) process. Hence, stem cells may be more suitable as nuclear donor cells for NT procedures than are somatic cells. Embryonic germ (EG) cells are undifferentiated stem cells that are isolated from cultured primordial germ cells (PGC) and can differentiate into several cell types. In this study, the in vitro development of NT embryos using porcine EG cells and their derivative neural precursor (NP) cells was investigated, thus eliminating any variation in genetic differences. The rates of fusion did not differ between NT embryos from EG and NP cells; however, the rate of cleavage in NT embryos derived from EG cells was significantly higher (p < 0.05) than that from NP cells (141/247 [57.1%] vs. 105/228 [46.1%]). Similarly, the rate of blastocyst development was significantly higher (P < 0.05) in NT using EG cells than the rate using NP cells (43/247 [17.4%] vs. 18/228 [7.9%]). The results obtained from the present study in pigs demonstrate a reduced capability for nuclear donor cells to be reprogrammed following the differentiation of porcine EG cells. Undifferentiated EG cells may be more amenable to reprogramming after reconstruction compared with differentiated somatic cells.

Keywords

Embryonic germ cellDifferentiationNeural precursor cellNuclear transferPig
Type
Research Article
Information
Zygote , Volume 20 , Issue 1 , February 2012 , pp. 9 - 15
Copyright
Copyright © Cambridge University Press 2011

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