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Impact of the number of retrieved oocytes on IVF outcomes: oocyte maturation, fertilization, embryo quality and implantation rate

Published online by Cambridge University Press:  19 December 2022

M. Jamil Open the ORCID record for M. Jamil [Opens in a new window] ,
H. Debbarh ,
A. Kabit ,
M. Ennaji ,
M. Zarqaoui ,
W. R. Senhaji ,
M. Hissane ,
B. Saadani ,
N. Louanjli  and
R. Cadi
M. Jamil*
Affiliation:
Laboratory of Molecular Genetic Physiopathology and Biotechnology. Department of Biology, Ain Chock Faculty of Sciences, HASSAN II University, Casablanca, Morocco IVF Center IRIFIV, Iris Clinic, Casablanca, Morocco
H. Debbarh
Affiliation:
Laboratory of Molecular Genetic Physiopathology and Biotechnology. Department of Biology, Ain Chock Faculty of Sciences, HASSAN II University, Casablanca, Morocco
A. Kabit
Affiliation:
IVF Center IRIFIV, Iris Clinic, Casablanca, Morocco
M. Ennaji
Affiliation:
IVF Center IRIFIV, Iris Clinic, Casablanca, Morocco
M. Zarqaoui
Affiliation:
IVF Center IRIFIV, Iris Clinic, Casablanca, Morocco
W. R. Senhaji
Affiliation:
IVF Center IRIFIV, Iris Clinic, Casablanca, Morocco
M. Hissane
Affiliation:
IVF Center IRIFIV, Iris Clinic, Casablanca, Morocco
B. Saadani
Affiliation:
IVF Center IRIFIV, Iris Clinic, Casablanca, Morocco
N. Louanjli
Affiliation:
IVF Center IRIFIV, Iris Clinic, Casablanca, Morocco
R. Cadi
Affiliation:
Laboratory of Molecular Genetic Physiopathology and Biotechnology. Department of Biology, Ain Chock Faculty of Sciences, HASSAN II University, Casablanca, Morocco
*
Author for correspondence: M. Jamil. Laboratory of Molecular Genetic Physiopathology and Biotechnology. Department of Biology, Ain Chock Faculty of Sciences, HASSAN II University, Casablanca, Morocco. E-mail: [email protected]
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Summary

The process of oocyte retrieval represents a key phase during the cycles of in vitro fertilization (IVF). It involves controlled ovarian stimulation to retrieve the highest number of oocytes possible. According to many previous studies, the higher the number of oocytes the higher the chances of obtaining embryos for multiple transfers. In this study, in total, 1987 patients were retrospectively reviewed to investigate the correlations between the number of retrieved oocytes and the subsequent IVF outcomes. Patients were divided into three groups according to the number of retrieved oocytes (Group 1: ≤5 oocytes; Group 2: 6–15 oocytes; Group 3: ≥15 oocytes). The results showed a significant negative correlation between oocyte number and maturation rate as well as fertilization rate. However, a significant positive correlation was found between oocyte number and the blastulation rate. The implantation rate after fresh embryo transfers was higher in group 2 (6–15 oocytes) compared with group 1 (≤5 oocytes). According to our findings, we conclude that oocyte numbers between 6 and 15 oocytes can result in the highest chances of positive IVF outcomes in terms of embryo quality and fresh embryo transfers with lower risks of ovarian hyperstimulation.

Keywords

BlastulationEmbryo qualityImplantationIn vitro fertilizationOocyte retrieval
Type
Research Article
Information
Zygote , Volume 31 , Issue 1 , February 2023 , pp. 91 - 96
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Copyright
© The Author(s), 2022. Published by Cambridge University Press

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References

Agrawal, S., Panchal, S. and Nagori, C. (2020). Relationship between follicular volume, oocyte competence, and blastocyst development in ART. Donald School Journal of Ultrasound in Obstetrics and Gynecology, 14(2), 136–143. doi: 10.5005/jp-journals-10009-1636 Google Scholar
Arya, B. K., Haq, A. U. and Chaudhury, K. (2012). Oocyte quality reflected by follicular fluid analysis in poly cystic ovary syndrome (PCOS): a hypothesis based on intermediates of energy metabolism. Medical Hypotheses, 78(4), 475–478. doi: 10.1016/j.mehy.2012.01.009 CrossRefGoogle ScholarPubMed
Behl, R. and Pandey, R. S. (2002). FSH induced stimulation of catalase activity in goat granulosa cells in vitro . Animal Reproduction Science, 70(3–4), 215–221. doi: 10.1016/s0378-4320(02)00006-4 CrossRefGoogle ScholarPubMed
Biljan, M. M., Buckett, W. M., Dean, N., Phillips, S. J. and Tan, S. L. (2000). The outcome of IVF-embryo transfer treatment in patients who develop three follicles or less. Human Reproduction, 15(10), 2140–2144. doi: 10.1093/humrep/15.10.2140 CrossRefGoogle ScholarPubMed
Bosch, E., Labarta, E., Kolibianakis, E., Rosen, M. and Meldrum, D. (2016). Regimen of ovarian stimulation affects oocyte and therefore embryo quality. Fertility and Sterility, 105(3), 560–570. doi: 10.1016/j.fertnstert.2016.01.022 CrossRefGoogle ScholarPubMed
Briggs, R., Kovacs, G., MacLachlan, V., Motteram, C. and Baker, H. W. G. (2015). Can you ever collect too many oocytes? Human Reproduction, 30(1), 81–87. doi: 10.1093/humrep/deu272 CrossRefGoogle ScholarPubMed
Cai, Q., Wan, F., Huang, K. and Zhang, Hanwang. (2013). Does the number of oocytes retrieved influence pregnancy after fresh embryo transfer? PLOS ONE, 8(2), e56189. doi: 10.1371/journal.pone.0056189 CrossRefGoogle ScholarPubMed
Cohen, Y., Tannus, Samer, Alzawawi, N., Son, W. Y., Dahan, M. and Buckett, W. (2018). Poor ovarian response as a predictor for live birth in older women undergoing IVF. Reproductive Biomedicine Online, 36(4), 435–441. doi: 10.1016/j.rbmo.2018.01.008 CrossRefGoogle ScholarPubMed
Das, M., Djahanbakhch, O., Hacihanefioglu, B., Saridogan, E., Ikram, M., Ghali, L., Raveendran, M. and Storey, A. (2008). Granulosa cell survival and proliferation are altered in polycystic ovary syndrome. Journal of Clinical Endocrinology and Metabolism, 93(3), 881–887. doi: 10.1210/jc.2007-1650 CrossRefGoogle ScholarPubMed
De Sutter, P. and Dhont, M. (2003). Poor response after hormonal stimulation for in vitro fertilization is not related to ovarian aging. Fertility and Sterility, 79(6), 1294–1298. doi: 10.1016/s0015-0282(03)00264-4 CrossRefGoogle Scholar
Decanter, C. (2018). Oocyte quality in PCOS. In: Palomba, S. (eds) Infertility in Women with Polycystic Ovary Syndrome. Springer, Cham. Available at https://doi.org/10.1007/978-3-319-45534-1_4 Google Scholar
Dolgushina, N. V., Syrkasheva, A. G., Makarova, N. P., Kovalskaya, E. V., Kalinina, E. A. and Sukhikh, G. T. (2015). Correlation between oocyte morphology and the embryo aneuploidy rate in IVF cycles. Gynecological Endocrinology, 31(Suppl. 1), 61–64. doi: 10.3109/09513590.2015.1086511 CrossRefGoogle Scholar
Dumesic, D. A. and Abbott, D. H. (2008). Implications of polycystic ovary syndrome on oocyte development. Seminars in Reproductive Medicine, 26(1), 53–61. doi: 10.1055/s-2007-992925 CrossRefGoogle ScholarPubMed
Fernández-González, R., Laguna, R., Ramos-Ibeas, P., Pericuesta, E., Alcalde-Lopez, V., Perez-Cerezales, S. and Gutierrez-Adan, A. (2019). Successful ICSI in mice using caput epididymal spermatozoa. Frontiers in Cell and Developmental Biology, 7, 346. doi: 10.3389/fcell.2019.00346 CrossRefGoogle ScholarPubMed
Graham, J., Han, T., Porter, R., Levy, M., Stillman, R. and Tucker, M. J. (2000). Day 3 morphology is a poor predictor of blastocyst quality in extended culture. Fertility and Sterility, 74(3), 495–497. doi: 10.1016/s0015-0282(00)00689-0 CrossRefGoogle ScholarPubMed
Gross, N., Kropp, J. and Khatib, H. (2017). MicroRNA signaling in embryo development. Biology, 6(3), 34. doi: 10.3390/biology6030034 CrossRefGoogle ScholarPubMed
Hendriks, D. J., Te Velde, E. R., Looman, C. W. N., Bancsi, L. F. J. M. M. and Broekmans, F. J. M. (2008). Expected poor ovarian response in predicting cumulative pregnancy rates: A powerful tool. Reproductive Biomedicine Online, 17(5), 727–736. doi: 10.1016/s1472-6483(10)60323-9 CrossRefGoogle ScholarPubMed
Homburg, R. (2009). Androgen circle of polycystic ovary syndrome. Human Reproduction, 24(7), 1548–1555. doi: 10.1093/humrep/dep049 CrossRefGoogle ScholarPubMed
Huang, B., Qian, K., Li, Z., Yue, J., Yang, W., Zhu, G. and Zhang, Hanwang. (2015). Neonatal outcomes after early rescue intracytoplasmic sperm injection: An analysis of a 5-year period. Fertility and Sterility, 103(6), 1432–7.e1. doi: 10.1016/j.fertnstert.2015.02.026 CrossRefGoogle ScholarPubMed
Jamil, M., Debbarh, H., Aboulmaouahib, S., Aniq Filali, O., Mounaji, K., Zarqaoui, M., Saadani, B., Louanjli, N. and Cadi, R. (2020). Reactive oxygen species in reproduction: Harmful, essential or both? Zygote, 28(4), 255–269. doi: 10.1017/S0967199420000179 CrossRefGoogle ScholarPubMed
Ji, J., Liu, Y., Tong, X. H., Luo, L., Ma, J. and Chen, Z. (2013). The optimum number of oocytes in IVF treatment: An analysis of 2455 cycles in China. Human Reproduction, 28(10), 2728–2734. doi: 10.1093/humrep/det303 CrossRefGoogle ScholarPubMed
Jonard, S. and Dewailly, D. (2004). The follicular excess in polycystic ovaries, due to intra-ovarian hyperandrogenism, may be the main culprit for the follicular arrest. Human Reproduction Update, 10(2), 107–117. doi: 10.1093/humupd/dmh010 CrossRefGoogle ScholarPubMed
Jones, C. A., Acharya, K. S., Acharya, C. R., Raburn, D. and Muasher, S. J. (2020). Patient and in vitro fertilization (IVF) cycle characteristics associated with variable blastulation rates: A retrospective study from the Duke Fertility Center (2013–2017). Middle East Fertility Society Journal, 24(1), 2–7. doi: 10.1186/s43043-019-0004-z CrossRefGoogle Scholar
Karakaya, C., Guzeloglu-Kayisli, O., Uyar, A., Kallen, A. N., Babayev, E., Bozkurt, N., Unsal, E., Karabacak, O. and Seli, E. (2015). Poor ovarian response in women undergoing in vitro fertilization is associated with altered microRNA expression in cumulus cells. Fertility and Sterility, 103(6), 1469–76.e1. doi: 10.1016/j.fertnstert.2015.02.035 CrossRefGoogle ScholarPubMed
Kenigsberg, S., Bentov, Y., Chalifa-Caspi, V., Potashnik, G., Ofir, R. and Birk, O. S. (2009). Gene expression microarray profiles of cumulus cells in lean and overweight-obese polycystic ovary syndrome patients. Molecular Human Reproduction, 15(2), 89–103. doi: 10.1093/molehr/gan082 CrossRefGoogle ScholarPubMed
Klinkert, E. R., Broekmans, F. J. M., Looman, C. W. N. and Te Velde, E. R. (2004). A poor response in the first in vitro fertilization cycle is not necessarily related to a poor prognosis in subsequent cycles. Fertility and Sterility, 81(5), 1247–1253. doi: 10.1016/j.fertnstert.2003.10.030 CrossRefGoogle Scholar
Kwon, H., Choi, D. H., Bae, J. H., Kim, J. H. and Kim, Y. S. (2010). MRNA expression pattern of insulin-like growth factor components of granulosa cells and cumulus cells in women with and without polycystic ovary syndrome according to oocyte maturity. Fertility and Sterility, 94(6), 2417–2420. doi: 10.1016/j.fertnstert.2010.03.053 CrossRefGoogle ScholarPubMed
Lai, Q., Xiang, W., Li, Q., Zhang, H., Li, Y., Zhu, G., Xiong, C. and Jin, L. (2018). Oxidative stress in granulosa cells contributes to poor oocyte quality and IVF-ET outcomes in women with polycystic ovary syndrome. Frontiers of Medicine, 12(5), 518–524. doi: 10.1007/s11684-017-0575-y.CrossRefGoogle ScholarPubMed
Langley, M. T., Marek, D. M., Gardner, D. K., Doody, K. M. and Doody, K. J. (2001). Extended embryo culture in human assisted reproduction treatments. Human Reproduction, 16(5), 902–908. doi: 10.1093/humrep/16.5.902 CrossRefGoogle ScholarPubMed
Lee, C. I., Chen, C. H., Huang, C. C., Cheng, E. H., Chen, H. H., Ho, S. T., Lin, P.-Y. Y., Lee, M. S. and Lee, T. H. (2019). Embryo morphokinetics is potentially associated with clinical outcomes of single-embryo transfers in preimplantation genetic testing for aneuploidy cycles. Reproductive Biomedicine Online, 39(4), 569–579. doi: 10.1016/j.rbmo.2019.05.020 CrossRefGoogle ScholarPubMed
Lin, L. T., Vitale, S. G., Chen, S. N., Wen, Z. H., Tsai, H. W., Chern, C. U. and Tsui, K. H. (2018). Luteal phase ovarian stimulation may improve oocyte retrieval and oocyte quality in poor ovarian responders undergoing in vitro fertilization: Preliminary results from a single-center prospective pilot study. Advances in Therapy, 35(6), 847–856. doi: 10.1007/s12325-018-0713-1 CrossRefGoogle ScholarPubMed
Macklon, N. S., Stouffer, R. L., Giudice, L. C. and Fauser, B. C. J. M. (2006). The science behind 25 years of ovarian stimulation for in vitro fertilization. Endocrine Reviews, 27(2), 170–207. doi: 10.1210/er.2005-0015 CrossRefGoogle ScholarPubMed
Melado Vidales, L., Fernández-Nistal, A., Martínez Fernández, V., Verdú Merino, V., Bruna Catalán, I. and Bajo Arenas, J. M. (2017) Anti-Müllerian hormone levels to predict oocyte maturity and embryo quality during controlled ovarian hyperstimulation. Minerva Ginecologica, 69(3), 225–232. doi: 10.23736/S0026-4784.16.03958-7 Google ScholarPubMed
Migdal, C. and Serres, M. (2011). Reactive oxygen species and oxidative stress. Medecine Sciences, 27(4), 405–412. doi: 10.1051/medsci/2011274017.CrossRefGoogle ScholarPubMed
Minasi, M. G., Colasante, A., Riccio, T., Ruberti, A., Casciani, V., Scarselli, F., Spinella, F., Fiorentino, F., Varricchio, M. T. and Greco, E. (2016). Correlation between aneuploidy, standard morphology evaluation and morphokinetic development in 1730 biopsied blastocysts: A consecutive case series study. Human Reproduction, 31(10), 2245–2254. doi: 10.1093/humrep/dew183 CrossRefGoogle ScholarPubMed
Munné, S., Kaplan, B., Frattarelli, J. L., Child, T., Nakhuda, G., Shamma, F. N., Silverberg, K., Kalista, T., Handyside, A. H., Katz-Jaffe, M., Wells, D., Gordon, T., Stock-Myer, S., Willman, S. and Study Group, STAR. (2019). Preimplantation genetic testing for aneuploidy versus morphology as selection criteria for single frozen–thawed embryo transfer in good-prognosis patients: a multicenter randomized clinical trial. Fertility and Sterility, 112(6), 1071–1079.e7. doi: 10.1016/j.fertnstert.2019.07.1346 CrossRefGoogle ScholarPubMed
Nikbakht, R., Mohammadjafari, R., Rajabalipour, M. and Moghadam, M. T. (2021). Evaluation of oocyte quality in polycystic ovary syndrome patients undergoing ART cycles. Fertility Research and Practice, 7(1), 2. doi: 10.1186/s40738-020-00094-z CrossRefGoogle ScholarPubMed
Plachot, M., Belaisch-Allart, J., Mayenga, J. M., Chouraqui, A., Tesquier, A., Serkine, A. M., Boujenah, A. and Abirached, F. (2003). Oocyte and embryo quality in polycystic ovary syndrome. Gynecologie, Obstetrique et Fertilite, 31(4), 350–354. doi: 10.1016/s1297-9589(03)00059-6 CrossRefGoogle ScholarPubMed
Polyzos, N. P., Drakopoulos, P., Parra, J., Pellicer, A., Santos-Ribeiro, S., Tournaye, H., Bosch, E. and Garcia-Velasco, J. (2018). Cumulative live birth rates according to the number of oocytes retrieved after the first ovarian stimulation for in vitro fertilization/intracytoplasmic sperm injection: a multicenter multinational analysis including ∼15,000 women. Fertility and Sterility, 110(4), 661–670.e1. doi: 10.1016/j.fertnstert.2018.04.039 CrossRefGoogle Scholar
Qiao, J. and Feng, H. L. (2011). Extra- and intra-ovarian factors in polycystic ovary syndrome: Impact on oocyte maturation and embryo developmental competence. Human Reproduction Update, 17(1), 17–33. doi: 10.1093/humupd/dmq032 CrossRefGoogle ScholarPubMed
Rienzi, L., Balaban, B., Ebner, T. and Mandelbaum, J. (2012). The oocyte. Human Reproduction, 27(Suppl. 1), i2–21. doi: 10.1093/humrep/des200.CrossRefGoogle ScholarPubMed
Shrikhande, L., Shrikhande, B. and Shrikhande, A. (2020). AMH and its clinical implications. Journal of Obstetrics and Gynaecology of India, 70(5), 337–341. doi: 10.1007/s13224-020-01362-0 CrossRefGoogle ScholarPubMed
Sigala, J., Sifer, C., Dewailly, D., Robin, G., Bruyneel, Aude, Ramdane, N., Lefebvre-Khalil, V., Mitchell, V. and Decanter, C. (2015). Is polycystic ovarian morphology related to a poor oocyte quality after controlled ovarian hyperstimulation for intracytoplasmic sperm injection? Results from a prospective, comparative study. Fertility and Sterility, 103(1), 112–118. doi: 10.1016/j.fertnstert.2014.09.040 CrossRefGoogle ScholarPubMed
Steward, R. G., Lan, L., Shah, A. A., Yeh, J. S., Price, T. M., Goldfarb, J. M. and Muasher, S. J. (2014). Oocyte number as a predictor for ovarian hyperstimulation syndrome and live birth: An analysis of 256,381 in vitro fertilization cycles. Fertility and Sterility, 101(4), 967–973. doi: 10.1016/j.fertnstert.2013.12.026 CrossRefGoogle ScholarPubMed
Sun, B., Ma, Y., Li, L., Hu, L., Wang, F., Zhang, Y., Dai, S. and Sun, Y. (2021). Factors associated with ovarian hyperstimulation syndrome (OHSS) severity in women with polycystic ovary syndrome undergoing IVF/ICSI. Frontiers in Endocrinology, 11, 615957. doi: 10.3389/fendo.2020.615957.CrossRefGoogle ScholarPubMed
Sunkara, S. K., Rittenberg, V., Raine-Fenning, N., Bhattacharya, S., Zamora, J. and Coomarasamy, A. (2011). Association between the number of eggs and live birth in IVF treatment: an analysis of 400 135 treatment cycles. Human Reproduction, 26(7), 1768–1774. doi: 10.1093/humrep/der106 CrossRefGoogle ScholarPubMed
Thaker, R., Mishra, V., Gor, M., Agarwal, R., Sheth, H., Kapadia, P. and Kumar, S. (2020). The role of stimulation protocol, number of oocytes retrieved with respect to follicular fluid oxidative stress and IVF outcome. Human Fertility, 23(1), 23–31. doi: 10.1080/14647273.2018.1551630 CrossRefGoogle ScholarPubMed
Timeva, T., Milachich, T., Antonova, I., Arabaji, T., Shterev, A. and Omar, H. A. (2006). Correlation between number of retrieved oocytes and pregnancy rate after in vitro fertilization/intracytoplasmic sperm infection. TheScientificWorldJournal, 6, 686–690. doi: 10.1100/tsw.2006.145 CrossRefGoogle ScholarPubMed
Uyar, A., Torrealday, S. and Seli, E. (2013). Cumulus and granulosa cell markers of oocyte and embryo quality. Fertility and Sterility, 99(4), 979–997. doi: 10.1016/j.fertnstert.2013.01.129 CrossRefGoogle ScholarPubMed
Vermey, B. G., Chua, S. J., Zafarmand, M. H., Wang, R., Longobardi, S., Cottell, E., Beckers, F., Mol, B. W., Venetis, C. A. and D’Hooghe, T. (2019). Is there an association between oocyte number and embryo quality? A systematic review and meta-analysis. Reproductive Biomedicine Online, 39(5), 751–763. doi: 10.1016/j.rbmo.2019.06.013 CrossRefGoogle ScholarPubMed
Yilmaz, N., Inal, H. A., Gorkem, U., Sargin Oruc, A., Yilmaz, S. and Turkkani, A. (2016). Follicular fluid total antioxidant capacity levels in PCOS. Journal of Obstetrics and Gynaecology, 36(5), 654–657. doi: 10.3109/01443615.2016.1148683 CrossRefGoogle ScholarPubMed
Yin, H., Jiang, H., He, R., Wang, C., Zhu, J. and Cao, Z. (2019). Cumulative live birth rate of advanced-age women more than 40 with or without poor ovarian response. Taiwanese Journal of Obstetrics and Gynecology, 58(2), 201–205. doi: 10.1016/j.tjog.2019.01.006 CrossRefGoogle ScholarPubMed
Zhang, Y., Liu, L., Yin, T. L., Yang, J. and Xiong, C. L. (2017). Follicular metabolic changes and effects on oocyte quality in polycystic ovary syndrome patients. Oncotarget, 8(46), 80472–80480. doi: 10.18632/oncotarget.19058 CrossRefGoogle ScholarPubMed
Zhen, X. M., Qiao, J., Li, R., Wang, L. N. and Liu, P. (2008). The clinical analysis of poor ovarian response in in-vitro-fertilization embryo-transfer among Chinese couples. Journal of Assisted Reproduction and Genetics, 25(1), 17–22. doi: 10.1007/s10815-007-9187-9 CrossRefGoogle ScholarPubMed
Zhou, J., Wang, B., Hu, Y. and Sun, H. (2017). Association between the number of oocytes retrieved and cumulative live birth rate in women aged 35–40 years undergoing long GnRH agonist IVF/ICSI cycles. Archives of Gynecology and Obstetrics, 296(5), 1005–1012. doi: 10.1007/s00404-017-4503-9 CrossRefGoogle ScholarPubMed
Ziebe, S., Lundin, K., Janssens, R., Helmgaard, L., Arce, J. C. and MERIT (Menotrophin vs Recombinant FSH In Vitro Fertilisation Trial) Group. (2007). Influence of ovarian stimulation with HP-HMG or recombinant FSH on embryo quality parameters in patients undergoing IVF. Human Reproduction, 22(9), 2404–2413. doi: 10.1093/humrep/dem221 CrossRefGoogle ScholarPubMed