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IGF-I slightly improves nuclear maturation and cleavage rate of bovine oocytes exposed to acute heat shock in vitro

Published online by Cambridge University Press:  29 May 2014

Qi Meiyu ,
Di Liu  and
Zvi Roth
Qi Meiyu
Affiliation:
Heilongjiang Academy of Agricultural Sciences, Harbin, China.
Di Liu
Affiliation:
Heilongjiang Academy of Agricultural Sciences, Harbin, China.
Zvi Roth*
Affiliation:
Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, the Hebrew University, Rehovot 76100, Israel.
*
All correspondence to: Zvi Roth. Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, the Hebrew University, Rehovot 76100, Israel. Tel: + 972 8 9489103. Fax: + 972 8 9498552. e-mail: [email protected]
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Summary

An in vitro model of embryo production was used to examine the effects of insulin-like growth factor (IGF)-I on maturation and developmental competence of oocytes exposed to heat shock. Cumulus–oocyte complexes were matured at 38.5°C or exposed to acute heat shock (HS; 41.5°C), with or without 100 ng/ml IGF-I, for 22 h through in vitro maturation. The experimental groups were control (C), C + IGF-I, HS, and HS + IGF-I. Oocytes were fertilized at the end of maturation, and the proportion of cleaved embryos was recorded 44 h later. HS during maturation increased the proportion of TUNEL-positive oocytes (P < 0.05). HS did not have any effect on cortical granule translocation but impaired resumption of meiosis, expressed as a decreased proportion of oocytes with nuclei in metaphase I (P < 0.05) and metaphase II (MII; P < 0.05). HS decreased the proportion of oocytes that cleaved (P < 0.05), in particular those oocytes that further developed to 4-cell-stage embryos (P < 0.05). IGF-I alleviated, to some extent, the deleterious effects of HS on the oocytes as reflected by a reduced proportion of TUNEL-positive oocytes (P < 0.03). While not significant, IGF-I tended to increase the proportion of MII-stage oocytes (P < 0.08) and 4-cell-stage cleaved embryos (P < 0.06). Further examination is required to explore whether IGF-I also affects the developmental competence of oocytes exposed to HS.

Keywords

BovineCleavage rateHeat shockIGF-IOocyte maturation
Type
Research Article
Information
Zygote , Volume 23 , Issue 4 , August 2015 , pp. 514 - 524
Copyright
Copyright © Cambridge University Press 2014 

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