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Identification of phospholipase activity in Rhinella arenarum sperm extract capable of inducing oocyte activation

Published online by Cambridge University Press:  09 September 2013

Federico Bonilla ,
Carlos Minahk ,
María Teresa Ajmat ,
Graciela Sánchez Toranzo  and
Marta Inés Bühler
Federico Bonilla*
Affiliation:
Departamento de Biología del Desarrollo, Instituto Superior de Investigaciones Biológicas (INSIBIO), Chacabuco 461–2 ° piso, T4000ILI San Miguel de Tucumán, República Argentina.
Carlos Minahk
Affiliation:
Departamento de Química Biológica - Instituto Superior de Investigaciones Biológicas-INSIBIO. Universidad Nacional de Tucumán, Chacabuco 461 (4000), Tucumán, Argentina.
María Teresa Ajmat
Affiliation:
Departamento de Biología del Desarrollo-INSIBIO. Facultad de Bioquímica, Química y Farmacia. Universidad Nacional de Tucumán, Chacabuco 461 (4000), Tucumán, Argentina.
Graciela Sánchez Toranzo
Affiliation:
Departamento de Biología del Desarrollo-INSIBIO. Facultad de Bioquímica, Química y Farmacia. Universidad Nacional de Tucumán, Chacabuco 461 (4000), Tucumán, Argentina.
Marta Inés Bühler
Affiliation:
Departamento de Biología del Desarrollo-INSIBIO. Facultad de Bioquímica, Química y Farmacia. Universidad Nacional de Tucumán, Chacabuco 461 (4000), Tucumán, Argentina.
*
All correspondence to: Federico Bonilla. Departamento de Biología del Desarrollo, Instituto Superior de Investigaciones Biológicas (INSIBIO), Chacabuco 461–2 ° piso, T4000ILI San Miguel de Tucumán, República Argentina. Tel: +54381 4247752 int. 7093. Fax: +54 381 4247752 int. 7004. e-mail: [email protected]
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Summary

Egg activation, which includes cortical granule exocytosis, resumption and completion of meiosis and pronuclear formation culminates in the first mitotic cleavage. However, the mechanism through which the fertilizing sperm induces this phenomenon is still controversial. We investigated the effect of the microinjection of homologous sperm soluble fractions obtained by fast protein liquid chromatography (FPLC) from reacted sperm (without acrosome) and non-reacted sperm on the activation of Rhinella arenarum oocytes matured in vitro. The FPLC-purified sperm fraction obtained from reacted or non-reacted sperm is able to induce oocyte activation when it is microinjected. This fraction has a 24 kDa protein and showed phospholipase C (PLC) activity in vitro, which was inhibited by D-609 but not by n-butanol or neomycin, suggesting that it is a PLC that is specific for phosphatidylcholine (PC-PLC). The assays conducted using inhibitors of inositol triphosphate (IP3) and ryanodine receptors (RyRs) indicate that the fraction with biological activity would act mainly through the cADPr (cyclic ADP ribose) pathway. Moreover, protein kinase C (PKC) inhibition blocks the activation produced by the same fraction. Immunocytochemical studies indicate that this PC-PLC can be found throughout the sperm head.

Keywords

DiacylglycerolOocyte activationPC-PLCRhinella arenarumSperm factor
Type
Research Article
Information
Zygote , Volume 22 , Issue 4 , November 2014 , pp. 483 - 495
Copyright
Copyright © Cambridge University Press 2013 

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