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Grooves surrounding the micropyle decrease the inseminating dose in fish

Published online by Cambridge University Press:  01 December 2017

Matheus Pereira-Santos ,
Eduardo Shimoda ,
André Furugen Cesar de Andrade ,
Luciano Andrade Silva ,
Takafumi Fujimoto ,
José Augusto Senhorini ,
George Shigueki Yasui  and
Laura Satiko Okada Nakaghi
Matheus Pereira-Santos*
Affiliation:
Aquaculture Center, São Paulo State University, Via de Acesso Prof. Paulo Donato Castellane s/n, 14884–900, Jaboticabal-SP, Brazil.
Eduardo Shimoda
Affiliation:
Department of Pharmacy, Cândido Mendes University, Rua Anita Peçanha, 100, 28030–335, Campos dos Goytacazes-RJ, Brazil.
André Furugen Cesar de Andrade
Affiliation:
Department of Veterinary, University of São Paulo, Avenida Duque de Caxias Norte, 225, 13630-080, Pirassununga-SP, Brazil.
Luciano Andrade Silva
Affiliation:
Department of Veterinary, University of São Paulo, Avenida Duque de Caxias Norte, 225, 13630-080, Pirassununga-SP, Brazil.
Takafumi Fujimoto
Affiliation:
Faculty of Fisheries Sciences, Hokkaido University, 3–1-1 Minato-cho, 041–8611, Hakodate, Japan.
José Augusto Senhorini
Affiliation:
National Center for Research and Conservation of Continental Fish, Chico Mendes Institute of Biodiversity Conservation, Rodovia Pref. Euberto Nemésio Pereira de Godoy, Km 6, 5, CP 65, 13.630-970. Pirassununga, São Paulo, Brazil.
George Shigueki Yasui
Affiliation:
Department of Veterinary, University of São Paulo, Avenida Duque de Caxias Norte, 225, 13630-080, Pirassununga-SP, Brazil.
Laura Satiko Okada Nakaghi
Affiliation:
Aquaculture Center, São Paulo State University, Via de Acesso Prof. Paulo Donato Castellane s/n, 14884–900, Jaboticabal-SP, Brazil.
*
All correspondence to: Matheus Pereira-Santos. Aquaculture Center, São Paulo State University, Via de Acesso Prof. Paulo Donato Castellane s/n, 14884–900, Jaboticabal-SP, Brazil. E-mail: [email protected]
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Summary

In fish with external fertilization, sperm must reach the oocyte through the micropyle to enter the cytoplasm. Fertilization success is then influenced by characteristics of oocytes or sperm. In this study, we evaluated oocyte morphology and sperm motility parameters and their effects on the inseminating dose in a teleost fish Astyanax altiparanae. Interestingly, we found one of the lowest yet described inseminating doses in teleosts (2390 spermatozoa oocyte−1 ml−1). Such a fertilization efficacy may be explained by the long duration of sperm motility (>75 s), the small oocyte diameter (695.119 µm), large micropyle diameter (7.57 µm), and the presence of grooves on the oocyte surface that guides spermatozoon to the fertilization area. Additionally, we have described for the first time a structure that combines grooves on the chorion surface and a ridge in the micropylar area.

Keywords

FertilizationGametesMorphologySpermTeleosts
Type
Research Article
Information
Zygote , Volume 25 , Issue 6 , December 2017 , pp. 731 - 739
Copyright
Copyright © Cambridge University Press 2017 

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