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Glyphosate decreases bovine oocyte quality by inducing oxidative stress and apoptosis

Published online by Cambridge University Press:  09 June 2022

Zhiqiang E Open the ORCID record for Zhiqiang E [Opens in a new window] ,
Yuhan Zhao Open the ORCID record for Yuhan Zhao [Opens in a new window] ,
Jingyu Sun ,
Xiaomeng Zhang ,
Qingguo Jin  and
Qingshan Gao
Zhiqiang E
Affiliation:
Engineering Research Center of North-East Cold Region Beef Cattle Science & Technology Innovation, Ministry of Education, Yanbian University, Yanji, 133002, China College of Agriculture, Yanbian University, China Jilin Engineering Research Center of Yanbian Yellow Cattle Resources Reservation, China
Yuhan Zhao
Affiliation:
Engineering Research Center of North-East Cold Region Beef Cattle Science & Technology Innovation, Ministry of Education, Yanbian University, Yanji, 133002, China College of Agriculture, Yanbian University, China Jilin Engineering Research Center of Yanbian Yellow Cattle Resources Reservation, China
Jingyu Sun
Affiliation:
Engineering Research Center of North-East Cold Region Beef Cattle Science & Technology Innovation, Ministry of Education, Yanbian University, Yanji, 133002, China College of Agriculture, Yanbian University, China Jilin Engineering Research Center of Yanbian Yellow Cattle Resources Reservation, China
Xiaomeng Zhang
Affiliation:
Engineering Research Center of North-East Cold Region Beef Cattle Science & Technology Innovation, Ministry of Education, Yanbian University, Yanji, 133002, China College of Agriculture, Yanbian University, China Jilin Engineering Research Center of Yanbian Yellow Cattle Resources Reservation, China
Qingguo Jin
Affiliation:
Engineering Research Center of North-East Cold Region Beef Cattle Science & Technology Innovation, Ministry of Education, Yanbian University, Yanji, 133002, China College of Agriculture, Yanbian University, China Jilin Engineering Research Center of Yanbian Yellow Cattle Resources Reservation, China
Qingshan Gao*
Affiliation:
Engineering Research Center of North-East Cold Region Beef Cattle Science & Technology Innovation, Ministry of Education, Yanbian University, Yanji, 133002, China College of Agriculture, Yanbian University, China Jilin Engineering Research Center of Yanbian Yellow Cattle Resources Reservation, China
*
Author for correspondence: Qingshan Gao. Departamento College of Agriculture, Yanbian University. Tel: +86 433 243 6435. E-mail: [email protected]
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Summary

Glyphosate is a universal herbicide with genital toxicity, but the effect of glyphosate on oocytes has not been reported. This study aimed to evaluate the effect of glyphosate (0, 10, 20, 50 and 100 mM) on bovine oocyte in vitro maturation. We showed that 50 mM glyphosate adversely affects the development of bovine oocytes. Exposure of oocytes to 50 mM glyphosate caused an abnormal reduction in oxidative (redox) levels compared with that in the control group, with a significantly higher reactive oxide species level (P < 0.05) and significantly lower glutathione (GSH) expression (P < 0.05). Additionally, the mRNA levels of antioxidant genes (SOD1, SOD2, SIRT2, SIRT3) and the mitochondrial membrane potential (MMP) were significantly reduced (P < 0.05). Furthermore, treatment with 50 mM glyphosate-induced apoptosis, and the mRNA levels of the apoptotic genes Caspase-3 and Caspase-4 were significantly higher than those in the control group (P < 0.05); however, the mRNA level of BAX was significantly higher than that in the control group (P < 0.01). Additionally, the mRNA levels of the anti-apoptotic genes Survivin and BCL-XL were significantly lower than those in the control group (P < 0.05), and oocyte quality was adversely affected. Together, our results confirmed that glyphosate impairs the quality of oocytes by promoting abnormal oocyte redox levels and apoptosis.

Keywords

BovineGlyphosateMitochondrialOocyteOxidative stress
Type
Research Article
Information
Zygote , Volume 30 , Issue 5 , October 2022 , pp. 704 - 711
Copyright
© The Author(s), 2022. Published by Cambridge University Press

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