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Full term development of normal mice after transfer of IVF embryos derived from oocytes stored at room temperature for 1 day

Published online by Cambridge University Press:  01 February 2008

Zi-Li Lei ,
Jun-Cheng Huang ,
Li-Hong Shi ,
Yi-Liang Miao ,
Chang-Long Nan ,
Ji-Wen Yang ,
Ying-Chun OuYang ,
Qing-Yuan Sun  and
Da-Yuan Chen
Zi-Li Lei
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, PR China. Graduate School, Chinese Academy of Sciences, Beijing 100039, PR China.
Jun-Cheng Huang
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, PR China. Graduate School, Chinese Academy of Sciences, Beijing 100039, PR China.
Li-Hong Shi
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, PR China. Graduate School, Chinese Academy of Sciences, Beijing 100039, PR China.
Yi-Liang Miao
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, PR China. Graduate School, Chinese Academy of Sciences, Beijing 100039, PR China.
Chang-Long Nan
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, PR China. Graduate School, Chinese Academy of Sciences, Beijing 100039, PR China.
Ji-Wen Yang
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, PR China. Graduate School, Chinese Academy of Sciences, Beijing 100039, PR China.
Ying-Chun OuYang
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, PR China.
Qing-Yuan Sun
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, PR China.
Da-Yuan Chen*
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, PR China.
*
1 All correspondence to: Da-Yuan Chen, State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, #25 Bei Si Huan Xi Road, Haidian, Beijing 100080, PR China. Tel: +86 10 64807052. Fax: +86 10 64807099. e-mail: [email protected]
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Summary

Early studies have shown that some mouse cumulus–oocyte complexes (COCs) stored at room temperature for 24 h still retained full developmental potential. In this study, we stored denuded mouse oocytes (DOs) at room temperature (25 °C) for 24 h and activated these oocytes with 10 mM SrCl2 or fertilized the oocytes by IVF. We found that nearly half of the DOs stored at room temperature for 1 day can be fertilized normally by IVF and that two foster mothers gave birth to seven pups. Embryos from stored oocytes were cultured in CZB medium with or without 1 μg/ml 17β-estradiol (E2). The numbers of embryo that developed to morula/blastocyst stage after parthenogenetic activation and IVF were significantly increased when E2 was added to the culture (p < 0.05). These results suggest that E2 might improve mouse embryo development in vitro. The birth of seven agouti pups and their healthy growth indicated that the storage of DOs at room temperature for 1 day may be a practical procedure for mammalian reproduction.

Keywords

Embryo development17β-EstradiolIVFOocytePup
Type
Research Article
Information
Zygote , Volume 16 , Issue 1 , February 2008 , pp. 21 - 27
Copyright
Copyright © Cambridge University Press 2008

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