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Fertilization and early embryonic development of in vitro matured metaphase I oocytes in patients with unexpected low oocyte maturity rate

Published online by Cambridge University Press:  01 October 2021

Nafiye Yılmaz ,
Şebnem Özyer Open the ORCID record for Şebnem Özyer [Opens in a new window] ,
Derya Taş ,
Mehmet Caner Özer ,
Ayten Türkkanı ,
Esra Şafak Yılmaz  and
Özlem Moraloğlu Tekin
Nafiye Yılmaz
Affiliation:
University of Health Sciences Turkey, Ankara City Hospital, Department of Assisted Reproductive Technologies, Obstetrics and Gynecology, Ankara, Turkey
Şebnem Özyer*
Affiliation:
University of Health Sciences Turkey, Ankara City Hospital, Department of Assisted Reproductive Technologies, Obstetrics and Gynecology, Ankara, Turkey
Derya Taş
Affiliation:
Ankara City Hospital, Department of Assisted Reproductive Technologies, Embryology, Ankara, Turkey
Mehmet Caner Özer
Affiliation:
Ankara City Hospital, Department of Assisted Reproductive Technologies, Embryology, Ankara, Turkey
Ayten Türkkanı
Affiliation:
University of Health Sciences Turkey, Ankara City Hospital, Department of Assisted Reproductive Technologies, Embryology, Ankara, Turkey
Esra Şafak Yılmaz
Affiliation:
University of Health Sciences Turkey, Gülhane Faculty of Medicine, Department of Biostatistics, Ankara, Turkey
Özlem Moraloğlu Tekin
Affiliation:
University of Health Sciences Turkey, Ankara City Hospital, Department of Assisted Reproductive Technologies, Obstetrics and Gynecology, Ankara, Turkey
*
Author for correspondence: Şebnem Özyer. Ankara City Hospital, Üniversiteler, Bilkent Blv. No. 1, 06800 Çankaya/Ankara/Turkey. Tel: +90 312 5526000. E-mail: [email protected]
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Summary

To determine the fertilization and embryonic potential of immature metaphase I (MI) oocytes in patients with low oocyte maturity rate in whom the percentage of mature oocytes obtained was less than 75% of the total retrieved ones. In vivo matured metaphase II (MII) oocytes (MII-ICSI, n = 244), and in vitro matured MI oocytes (MI-MII-ICSI, n = 202) underwent an intracytoplasmic sperm injection (ICSI) procedure. Maturation rate, fertilization rate and early embryonic development were compared in both groups. In total, 683 oocytes were collected from 117 ICSI cycles of 117 patients. Among them, 244 (35.7%) were mature MII and 259 (37.9%) were MI after the denudation process. Of those 259 MI oocytes, 202 (77.9%) progressed to MII oocytes after an incubation period of 18–24 h. The maturation rate was 77.9%. Fertilization rate was found to be significantly higher in the rescued in vitro matured MI oocyte group when compared with the in vivo matured MII oocyte group (41.6% vs 25.8%; P = 0.0006). However, no significant difference was observed in terms of cleavage rates on days 2 and 3 between the groups (P = 0.9126 and P = 0.5031, respectively). There may be unidentified in vivo factors on the oocyte maturation causing low developmental capacity in spite of high fertilization rates in the group of patients with low oocyte maturity rate. Furthermore, studies are needed to determine the appropriate culture characteristics as well as culture period and ICSI timing of these oocytes.

Keywords

FertilizationMaturationMetaphase IOocyteOocyte maturity rate
Type
Research Article
Information
Zygote , Volume 30 , Issue 3 , June 2022 , pp. 319 - 323
Copyright
© The Author(s), 2021. Published by Cambridge University Press

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