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Fertilisation competence of bovine normally matured or aged oocytes derived from different antral follicles: morphology, protein synthesis, H1 and MBP kinase activity

Published online by Cambridge University Press:  26 September 2008

Antonín Pavlok ,
Petr Kaláb  and
Petr Bobák
Antonín Pavlok*
Affiliation:
Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Libeˇchov, Czech Republic
Petr Kaláb
Affiliation:
Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Libeˇchov, Czech Republic
Petr Bobák
Affiliation:
Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Libeˇchov, Czech Republic
*
Dr Antonín Pavlok, Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, CZ-277 21 Liběchov, Czech Republic. Tel: 420 206 697147. Fax: 420 206 697186. e-mail: [email protected].
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Summary

We have investigated the fertilisation competence, protein synthesis, histone H1 kinase and myelin basic protein (MBP) kinase activities in three categories of bovine oocytes (derived from three size categories of follicles: M–medium, 2.5–5.0 mm; S–small, 1.5–2.5 mm; T – tiny, 1.0–1.5mm). In contrast to more or less normal meiotic maturation (85.6%) and fertilisation (70.8%) of M oocytes cultured for 24h, the fertilisation of M oocytes cultured for 40h was associated with increased rates of retarded male pronuclear development and retention of the second polar body. The S and T oocytes cultured for 24h or 40h were mostly arrested at defective late diakinesis - metaphase I (77.5–100%) stage. After fertilisation of S and T oocytes cultured for 24h no polar body was extruded and formation of one, three or four female pronuclei, together with mostly normal male pronuclei, was observed. The fertilisation of S and T oocytes after 40h culture resulted in a higher number of female and a decreased number of male pronuclei. A major change in the pattern of protein synthesis was associated with the resumption of meiosis. There were no significant differences in the profile of protein synthesis between oocyte categories in all groups either matured or fertilised. The H1 kinase activity reached comparable increased levels in oocytes of all categories matured for 24h and decreased during the 40h culture, most significantly in M oocytes. The MBP kinase activity was at approximately the same high level in all categories of oocytes after 24h of culture and remained stable until 40h. The fertilisation after 24h of culture resulted, in M oocytes, in low levels of both H1 and MBP kinase activities; in S oocytes, only H1 kinase was completely inactivated while MBP kinase activity decreased to some extent; in T oocytes, both H1 and MBP kinase activity decreased. Fertilisation of all oocyte categories after 40h culture resulted in complete inactivation of both these kinases to their basal levels.

Keywords

FertilisationHistone H1 kinaseMBP kinaseOocyte maturationOocyte protein synthesis
Type
Article
Information
Zygote , Volume 5 , Issue 3 , August 1997 , pp. 235 - 246
Copyright
Copyright © Cambridge University Press 1997

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