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Expression of follicle-stimulating hormone receptor (FSHR) in goat ovarian follicles and the impact of sequential culture medium on in vitro development of caprine preantral follicles

Published online by Cambridge University Press:  21 December 2010

M.V.A. Saraiva ,
J.J.H. Celestino ,
V.R. Araújo ,
R.N. Chaves ,
A.P. Almeida ,
I.B. Lima-Verde ,
A.B.G. Duarte ,
G.M. Silva ,
F.S. Martins  and
J.B. Bruno
...Show all authors
M.V.A. Saraiva*
Affiliation:
Programa de Pós-Graduação em Ciências Veterinárias (PPGCV), Laboratório de Manipulação de Oócitos e Folículos Pré-Antrais (LAMOFOPA), Universidade Estadual do Ceará (UECE), Av. Paranjana, 1700, Campus do Itaperi, Fortaleza–CE–Brasil, CEP: 60740–000.
J.J.H. Celestino
Affiliation:
Faculty of Veterinary Medicine, LAMOFOPA, PPGCV, State University of Ceara, Fortaleza-CE, Brazil.
V.R. Araújo
Affiliation:
Faculty of Veterinary Medicine, LAMOFOPA, PPGCV, State University of Ceara, Fortaleza-CE, Brazil.
R.N. Chaves
Affiliation:
Faculty of Veterinary Medicine, LAMOFOPA, PPGCV, State University of Ceara, Fortaleza-CE, Brazil.
A.P. Almeida
Affiliation:
Faculty of Veterinary Medicine, LAMOFOPA, PPGCV, State University of Ceara, Fortaleza-CE, Brazil.
I.B. Lima-Verde
Affiliation:
Faculty of Veterinary Medicine, LAMOFOPA, PPGCV, State University of Ceara, Fortaleza-CE, Brazil.
A.B.G. Duarte
Affiliation:
Faculty of Veterinary Medicine, LAMOFOPA, PPGCV, State University of Ceara, Fortaleza-CE, Brazil.
G.M. Silva
Affiliation:
Faculty of Veterinary Medicine, LAMOFOPA, PPGCV, State University of Ceara, Fortaleza-CE, Brazil.
F.S. Martins
Affiliation:
Faculty of Veterinary Medicine, LAMOFOPA, PPGCV, State University of Ceara, Fortaleza-CE, Brazil.
J.B. Bruno
Affiliation:
Faculty of Veterinary Medicine, LAMOFOPA, PPGCV, State University of Ceara, Fortaleza-CE, Brazil.
M.H.T. Matos
Affiliation:
Faculty of Veterinary Medicine, LAMOFOPA, PPGCV, State University of Ceara, Fortaleza-CE, Brazil.
C.C. Campello
Affiliation:
Faculty of Veterinary Medicine, LAMOFOPA, PPGCV, State University of Ceara, Fortaleza-CE, Brazil.
J.R.V. Silva
Affiliation:
Biotecnology Nucleus of Sobral (NUBIS), Federal University of Ceara, Sobral-CE, Brazil.
J.R. Figueiredo
Affiliation:
Faculty of Veterinary Medicine, LAMOFOPA, PPGCV, State University of Ceara, Fortaleza-CE, Brazil.
*
All correspondence to: M.V.A. Saraiva. Programa de Pós-Graduação em Ciências Veterinárias (PPGCV), Laboratório de Manipulação de Oócitos e Folículos Pré-Antrais (LAMOFOPA), Universidade Estadual do Ceará (UECE), Av. Paranjana, 1700, Campus do Itaperi, Fortaleza–CE–Brasil, CEP: 60740–000. Tel: +55 85 3101 9852; Fax: +55 85 3101 9840. e-mail address: [email protected]
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Summary

This study evaluated the expression of FSH receptors (FSHR) in the different stages of goat follicle development and investigated whether the addition of increasing concentrations of FSH throughout the culture period influences the survival, growth and antral formation of in vitro-cultured caprine preantral follicles. The expression of FSHR was analysed before and after culturing follicles using real-time RT-PCR. For the culture, preantral follicles (≥150 μm) were isolated from ovarian fragments and cultured for 18 days in α-MEM+ alone or associated with recombinant FSH (rFSH: 100 or 1000 ng/ml), or in α-MEM+ supplemented with increasing concentrations of FSH throughout culture periods as follows: (a) sequential medium 1: FSH 100 ng/ml (from day 0 to 6), FSH 500 ng/ml (from day 6 to 12) and FSH 1000 ng/ml (from day 12 to 18); and (b) sequential medium 2: FSH 500 ng/ml (from day 0 to 9) and 1000 ng/ml (from day 9 to 18). Follicle development was evaluated on the basis of antral cavity formation, follicular and oocyte growth, and cumulus–oocyte complex health. The expression of FSHR in isolated caprine follicles increased from the preantral to antral phase. Regarding the culture, after 18 days, sequential medium 1 promoted follicular survival, antrum formation and a reduction in oocyte extrusion. Both sequential media promoted a higher rate of meiotic resumption compared with the other treatments. In conclusion, the addition of increased concentrations of FSH (sequential medium) has a significant impact on the in vitro development of caprine preantral follicles.

Keywords

CultureFSH receptorGoatOvarian follicleSequential FSH
Type
Research Article
Information
Zygote , Volume 19 , Issue 3 , August 2011 , pp. 205 - 214
Copyright
Copyright © Cambridge University Press 2010

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