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Expression of Fas–Fas ligand system associated with atresia in murine ovary

Published online by Cambridge University Press:  26 September 2008

Ji Ping Xu ,
Xiang Li ,
Etsuko Mori ,
Eimei Sato ,
Shigeru Saito ,
Mao Wu Guo  and
Tsuneatsu Mori
Ji Ping Xu
Affiliation:
Institute of Medical Science, University of Tokyo, and Nara Medical University, Japan.
Xiang Li
Affiliation:
Institute of Medical Science, University of Tokyo, and Nara Medical University, Japan.
Etsuko Mori
Affiliation:
Institute of Medical Science, University of Tokyo, and Nara Medical University, Japan.
Eimei Sato
Affiliation:
Institute of Medical Science, University of Tokyo, and Nara Medical University, Japan.
Shigeru Saito
Affiliation:
Institute of Medical Science, University of Tokyo, and Nara Medical University, Japan.
Mao Wu Guo
Affiliation:
Institute of Medical Science, University of Tokyo, and Nara Medical University, Japan.
Tsuneatsu Mori*
Affiliation:
Institute of Medical Science, University of Tokyo, and Nara Medical University, Japan.
*
T. Mori, Department of Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 shirokanedai, Minato-ku, Tokyo 108, Japan. Telephone: +81-3-5449-5261. Fax: +81-3-5449-5402, e-mail: [email protected].
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Summary

We detected that Fas receptor (Fas) was expressed at transcriptional levels in oocytes/eggs and some granulosa cells of murine ovary, whereas, Fas ligand (FasL) was found to be strongly expressed in granulosa cells by means of in situ hybridisation. These results were supported by an indirect immunofluorescence (IIF) test with anti-Fas monoclonal antibody (mAb)/FasL Ab. The lysates from granulosa cells were precipitated by anti-FasL Ab, exhibiting a specific band at 40 kDa. When zona pellucida (ZP)-free eggs were incubated with granulosa cells in vitro, apoptosis (DNA fragmentation) was induced in the eggs, as detected by the terminal deoxynucleotide transferase mediated dUTP-nick end labelling (TUNEL) method. To examine whether FasL-bearing cells can induce apoptosis in eggs expressing Fas through molecular interaction between FasL and Fas, we employed a baculovirus expression system to generate FasL on the surface of Spodoptera frugiperda (Sf9) cells. The co-incubation of eggs with Autographa californica nuclear polyhedrosis virus (AcNPV) and FasL transfected Sf9 (Sf9–FasL) cells in vitro was performed and resulted in the induction of apoptosis in eggs as detected by the TUNEL method. Apoptosis was absent in eggs co-incubated with AcNPV-1393 transfected Sf9 (Sf9−1393) cells. Thus, ovarian atresia was suggested to be induced by molecular interaction between FasL on granulosa cells and Fas on oocytes/eggs during oogenesis.

Keywords

Fas–Fas ligandOocyte/eggGranulosa cellSF9-FasL cellAtresia
Type
Article
Information
Zygote , Volume 5 , Issue 4 , November 1997 , pp. 321 - 327
Copyright
Copyright © Cambridge University Press 1997

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