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Expression and functional activity of neurotransmitter system components in sea urchins’ early development†

Published online by Cambridge University Press:  29 April 2015

Denis A. Nikishin ,
Ivan Milošević ,
Milorad Gojković ,
Ljubisav Rakić ,
Vladimir V. Bezuglov  and
Yuri B. Shmukler
Denis A. Nikishin
Affiliation:
N.K. Koltzov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia. M.V. Lomonosov Moscow State University, Moscow, Russia.
Ivan Milošević
Affiliation:
Institute of Marine Biology, Kotor, Montenegro.
Milorad Gojković
Affiliation:
Institute of Marine Biology, Kotor, Montenegro.
Ljubisav Rakić
Affiliation:
Serbian Academy of Science and Art, Belgrade, Serbia.
Vladimir V. Bezuglov
Affiliation:
M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bio-Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Yuri B. Shmukler*
Affiliation:
26, Vavilov St, Moscow 119334, Russia N.K. Koltzov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia.
*
All correspondence to: Yuri B. Shmukler. 26, Vavilov St, Moscow 119334, Russia. Tel: +7 499 135 0052. Fax: +7 499 135 8012. E-mail: [email protected]
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Summary

Reverse-transcription polymerase chain reaction (RT-PCR) investigation of the expression of the components supposedly taking part in serotonin regulation of the early development of Paracentrotus lividus has shown the presence of transcripts of five receptors, one of which has conservative amino acid residues characteristic of monoaminergic receptors. At the early stages of embryogenesis the expressions of serotonin transporter (SERT) and noradrenaline transporter (NET) were also recognized. The activities of the enzymes of serotonin synthesis and serotonin transporter were shown using immunohistochemistry and incubation with para-chlorophenylalanine (PСРА) and 5-hydroxytryptophan (HTP). Pharmacological experiments have shown a preferential cytostatic activity of ligands characterized as mammalian 5-hydroxytryptamine (5-HT)1-antagonists. On the basis of the sum of the data from molecular biology and embryo physiological experiments, it is suggested that metabotropic serotonin receptors and membrane transporters take part in the regulatory processes of early sea urchin embryogenesis.

Keywords

EmbryoReceptorSea urchinSerotoninTransporter
Type
Research Article
Information
Zygote , Volume 24 , Issue 2 , April 2016 , pp. 206 - 218
Copyright
Copyright © Cambridge University Press 2015 

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Footnotes

†

In memory of Prof. Gennady A. Buznikov, pioneer of embryonic transmitter research.

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