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Exosc10 deficiency in the initial segment is dispensable for sperm maturation and male fertility in mice

Published online by Cambridge University Press:  18 November 2024

Meiyang Zhou ,
Junjie Yu ,
Yu Xu ,
Hong Li ,
Yan-Qin Feng ,
Xiao Wang ,
Fanyi Qiu ,
Nana Li  and
Zhengpin Wang
Meiyang Zhou
Affiliation:
Shandong Provincial Key Laboratory of Animal Cell and Developmental Biology, School of Life Sciences, Shandong University, Qingdao 266237, PR China
Junjie Yu
Affiliation:
Shandong Provincial Key Laboratory of Animal Cell and Developmental Biology, School of Life Sciences, Shandong University, Qingdao 266237, PR China
Yu Xu
Affiliation:
Shandong Provincial Key Laboratory of Animal Cell and Developmental Biology, School of Life Sciences, Shandong University, Qingdao 266237, PR China
Hong Li
Affiliation:
Shandong Provincial Key Laboratory of Animal Cell and Developmental Biology, School of Life Sciences, Shandong University, Qingdao 266237, PR China
Yan-Qin Feng
Affiliation:
Shandong Provincial Key Laboratory of Animal Cell and Developmental Biology, School of Life Sciences, Shandong University, Qingdao 266237, PR China
Xiao Wang
Affiliation:
Shandong Provincial Key Laboratory of Animal Cell and Developmental Biology, School of Life Sciences, Shandong University, Qingdao 266237, PR China
Fanyi Qiu
Affiliation:
Shandong Provincial Key Laboratory of Animal Cell and Developmental Biology, School of Life Sciences, Shandong University, Qingdao 266237, PR China
Nana Li*
Affiliation:
Shandong Provincial Key Laboratory of Animal Cell and Developmental Biology, School of Life Sciences, Shandong University, Qingdao 266237, PR China
Zhengpin Wang*
Affiliation:
Shandong Provincial Key Laboratory of Animal Cell and Developmental Biology, School of Life Sciences, Shandong University, Qingdao 266237, PR China
*
Corresponding author: Zhengpin Wang; Email: [email protected] or Nana Li; Email: [email protected]
Corresponding author: Zhengpin Wang; Email: [email protected] or Nana Li; Email: [email protected]
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Summary

EXOSC10 is an exosome-associated ribonuclease that degrades and processes a wide range of transcripts in the nucleus. The initial segment (IS) of the epididymis is crucial for sperm transport and maturation in mice by affecting the absorption and secretion that is required for male fertility. However, the role of EXOSC10 ribonuclease-mediated RNA metabolism within the IS in the regulation of gene expression and sperm maturation remains unknown. Herein, we established an Exosc10 conditional knockout (Exosc10 cKO) mouse model by crossing Exosc10F/F mice with Lcn9-Cre mice which expressed recombinase in the principal cells of IS as early as post-natal day 17. Morphological and histological analyses revealed that Exosc10 cKO males had normal spermatogenesis and development of IS. Moreover, the sperm concentration, morphology, motility, and frequency of acrosome reactions in the cauda epididymides of Exosc10 cKO mice were comparable with those of control mice. Thus, Exosc10 cKO males had normal fertility. Collectively, our genetic mouse model and findings demonstrate that loss of EXOSC10 in the IS of epididymis is dispensable for sperm maturation and male fertility.

Keywords

epididymisEXOSC10initial segmentspermatogenesissperm maturation
Type
Research Article
Information
Zygote , Volume 32 , Issue 6 , December 2024 , pp. 437 - 445
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Copyright
© The Author(s), 2024. Published by Cambridge University Press

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Footnotes

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These authors contributed equally to this work

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