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Epididymal tail solid-surface vitrification as an effective method for domestic cat sperm cryobanking

Published online by Cambridge University Press:  08 April 2021

Silmara L.G. Lima ,
Airton R.B. Soares ,
Leanne Stalker ,
Regiane R. Santos Open the ORCID record for Regiane R. Santos [Opens in a new window]  and
Sheyla F.S. Domingues Open the ORCID record for Sheyla F.S. Domingues [Opens in a new window]
Silmara L.G. Lima
Affiliation:
Laboratory of Wild Animal Biotechnology and Medicine, Faculty of Veterinary Medicine, Federal University of Pará, Castanhal, Pará, Brazil Postgraduate Program in Animal Health and Production in the Amazon, Federal Rural University of Amazonia, Belém, Pará, Brazil
Airton R.B. Soares
Affiliation:
Laboratory of Wild Animal Biotechnology and Medicine, Faculty of Veterinary Medicine, Federal University of Pará, Castanhal, Pará, Brazil
Leanne Stalker
Affiliation:
Department of Biomedical Science, University of Guelph, Guelph, Ontario, Canada
Regiane R. Santos*
Affiliation:
Laboratory of Wild Animal Biotechnology and Medicine, Faculty of Veterinary Medicine, Federal University of Pará, Castanhal, Pará, Brazil
Sheyla F.S. Domingues
Affiliation:
Laboratory of Wild Animal Biotechnology and Medicine, Faculty of Veterinary Medicine, Federal University of Pará, Castanhal, Pará, Brazil Postgraduate Program in Animal Health and Production in the Amazon, Federal Rural University of Amazonia, Belém, Pará, Brazil Postgraduate Program of Animal Reproduction in Amazon, Institute of Veterinary Medicine, Federal University of Pará, Castanhal, Pará, Brazil
*
Author for correspondence: Regiane R. Santos. Laboratory of Wild Animal Biotechnology and Medicine, Federal University of Pará, BR 316 Km 61, CEP 68740–970, Castanhal, Pará, Brazil. Tel: +55 91 33114707. E-mail: [email protected]
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Summary

This study aimed to describe the viability of domestic feline spermatozoa after epididymal tail vitrification. For this, 10 pairs of testis–epididymis complexes were used. The epididymal tails were vitrified using the solid-surface vitrification (SSV) method, in which two vitrification media containing ethylene glycol (EG) 40% or glycerol (GLY) 40% were tested. Vitrification with the presence of EG resulted in better results for all sperm motility parameters (motility, vigour and CASA) compared with GLY (P < 0.05). There were no statistical differences for sperm viability and acrosome integrity, plasma membrane integrity, or overall health of morphologically normal sperm before or after vitrification among experimental groups. In conclusion, epididymal tail vitrification appears to be a suitable method for long-term storage of cat sperm, especially if the procedure is performed with EG as the cryoprotectant.

Keywords

CASAEpididymisFelineSpermatozoaVitrification
Type
Research Article
Information
Zygote , Volume 29 , Issue 6 , December 2021 , pp. 452 - 458
Copyright
© The Author(s), 2021. Published by Cambridge University Press

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