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EH domain binding protein 1-like 1 (EHBP1L1), a protein with calponin homology domain, is expressed in the rat testis

Published online by Cambridge University Press:  29 July 2020

Massimo Venditti Open the ORCID record for Massimo Venditti [Opens in a new window] ,
Aldo Donizetti ,
Francesco Aniello  and
Sergio Minucci Open the ORCID record for Sergio Minucci [Opens in a new window]
Massimo Venditti
Affiliation:
Dipartimento di Medicina Sperimentale, Sez. Fisiologia Umana e Funzioni Biologiche Integrate ‘F. Bottazzi’, Università degli Studi della Campania ‘Luigi Vanvitelli’ via Costantinopoli, 16-80138 – Napoli, Italy
Aldo Donizetti
Affiliation:
Dipartimento di Biologia, Università di Napoli ‘Federico II, via Cinthia’, 21-80126 – Napoli, Italy
Francesco Aniello*
Affiliation:
Dipartimento di Biologia, Università di Napoli ‘Federico II, via Cinthia’, 21-80126 – Napoli, Italy
Sergio Minucci*
Affiliation:
Dipartimento di Medicina Sperimentale, Sez. Fisiologia Umana e Funzioni Biologiche Integrate ‘F. Bottazzi’, Università degli Studi della Campania ‘Luigi Vanvitelli’ via Costantinopoli, 16-80138 – Napoli, Italy
*
Authors for correspondence: Sergio Minucci. Dipartimento di Medicina Sperimentale, Sez. Fisiologia Umana e Funzioni Biologiche Integrate ‘F. Bottazzi’, Università degli Studi della Campania ‘Luigi Vanvitelli’ via Costantinopoli, 16-80138 – Napoli, Italy. Tel: +39081-5665829. E-mail: [email protected]. Francesco Aniello. Dipartimento di Biologia, Università di Napoli ‘Federico II, via Cinthia’, 21-80126 – Napoli, Italy. Tel: +39081-2535014. E-mail: [email protected].
Authors for correspondence: Sergio Minucci. Dipartimento di Medicina Sperimentale, Sez. Fisiologia Umana e Funzioni Biologiche Integrate ‘F. Bottazzi’, Università degli Studi della Campania ‘Luigi Vanvitelli’ via Costantinopoli, 16-80138 – Napoli, Italy. Tel: +39081-5665829. E-mail: [email protected]. Francesco Aniello. Dipartimento di Biologia, Università di Napoli ‘Federico II, via Cinthia’, 21-80126 – Napoli, Italy. Tel: +39081-2535014. E-mail: [email protected].
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Summary

In this paper, with the aim to find new genes involved in mammalian spermatogenesis, we isolated, for the first time in the rat testis, a partial cDNA clone that encoded EH domain binding protein 1-like 1 (Ehbp1l1), a protein that has a single calponin homology domain (CH). Bioinformatic analysis showed that EHBP1l1 contains three domains: the N-terminal C2-like, the CH and the C-terminal bivalent Mical/EHBP Rab binding (bMERB) domains, which are evolutionarily conserved in vertebrates. We found that Ehbp1l1 mRNA was expressed in several rat tissues, including the liver, intestine, kidney and also in the testis during its development, with a higher level in testis from 12-month-old animals. Interestingly, in situ hybridization experiments revealed that Ehbp1l1 is specifically expressed by types I and II spermatocytes, this result was validated by RT-PCR performed on total RNA obtained from enriched fractions of different testicular cell types. As EHBP1l1 has been described as linked to vesicular transport to the actin cytoskeleton and as an effector of the small GTPase Rab8, we hypothesized that it could participate both in cytoskeletal remodelling and in the regulation of vesicle sorting from the trans-Golgi network to the apical plasma membrane. Our findings provide a better understand of the molecular mechanisms of the differentiation process of spermatogenesis; Ehbp1l1 may also be used as a new marker of testicular activity.

Keywords

ActinEhbp1l1MeiosisSpermatogenesisTestis
Type
Research Article
Information
Zygote , Volume 28 , Issue 6 , December 2020 , pp. 441 - 446
Copyright
© The Author(s), 2020. Published by Cambridge University Press

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Footnotes

*

In memory of our friend, Professor Anna Cardone.

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