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Efficiency and kinetics of the in vitro fertilization process in bovine oocytes with different meiotic competence

Published online by Cambridge University Press:  01 August 2007

J. Horakova ,
K. Hanzalova ,
Z. Reckova ,
P. Hulinska  and
M. Machatkova
J. Horakova
Affiliation:
Department of Genetics and Reproduction, Veterinary Research Institute, Hudcova, Brno, Czech Republic
K. Hanzalova
Affiliation:
Department of Genetics and Reproduction, Veterinary Research Institute, Hudcova, Brno, Czech Republic
Z. Reckova
Affiliation:
Department of Genetics and Reproduction, Veterinary Research Institute, Hudcova, Brno, Czech Republic
P. Hulinska
Affiliation:
Department of Genetics and Reproduction, Veterinary Research Institute, Hudcova, Brno, Czech Republic
M. Machatkova*
Affiliation:
Department of Genetics and Reproduction, Veterinary Research Institute, Hudcova, Brno, Czech Republic
*
All correspondence to: Marie Machatkova, Department of Genetics and Reproduction, Veterinary Research Institute, Hudcova 70, 621 32 Brno, Czech Republic. Tel: +420 533 331 418. Fax: +420 541 211 229. e-mail: [email protected]
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Summary

The aim of the study was to investigate the efficiency and kinetics of fertilization in oocytes with different meiotic competence, as defined by the phase of the follicular wave and follicle size. Oocytes were recovered from cows with synchronized estrus cycles, slaughtered in either the growth (day 3) or the dominant (day 7) phase, separately from large, medium and small follicles. The oocytes were matured and fertilized by a standard protocol. Twenty-four hours after fertilization, the oocytes were denuded from cumulus cells, fixed and stained with bisbensimid Hoechst–PBS. Fertilization was more efficient and the first cleavage was accelerated in growth phase-derived oocytes, as shown by significantly higher (p ≤ 0.01) proportions of both normally fertilized and cleaved oocytes (68.8 and 25.1%), in comparison with dominant phase-derived oocytes (44.2 and 10.3%). In the growth-phase derived oocytes, proportions of normally fertilized and cleaved oocytes were significantly higher (p ≤ 0.01) in oocytes from large (100.0 and 36.4%) and medium (83.3 and 36.5%) follicles than in those from small (54.8 and 14.6%) follicles. The dominant phase-derived oocytes showed higher proportions of normally fertilized and cleaved oocytes in the populations recovered from small (51.5 and 10.0%) and medium (43.1 and 12.0%) follicles than in those from large (25.0 and 0%) follicles; however, the differences were not significant. It can be concluded that: (i) efficiency and kinetics of fertilization differ in relation to oocyte's meiotic competence; (ii) improved development of embryos from oocytes with greater meiotic competence is associated with a more effective fertilization process.

Keywords

BovineEfficiencyIn vitro fertilizationKineticsOocyte
Type
Research Article
Information
Zygote , Volume 15 , Issue 3 , August 2007 , pp. 251 - 256
Copyright
Copyright © Cambridge University Press 2007

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