No CrossRef data available.
Article contents
Efficiency and kinetics of the in vitro fertilization process in bovine oocytes with different meiotic competence
Published online by Cambridge University Press: 01 August 2007
Summary
The aim of the study was to investigate the efficiency and kinetics of fertilization in oocytes with different meiotic competence, as defined by the phase of the follicular wave and follicle size. Oocytes were recovered from cows with synchronized estrus cycles, slaughtered in either the growth (day 3) or the dominant (day 7) phase, separately from large, medium and small follicles. The oocytes were matured and fertilized by a standard protocol. Twenty-four hours after fertilization, the oocytes were denuded from cumulus cells, fixed and stained with bisbensimid Hoechst–PBS. Fertilization was more efficient and the first cleavage was accelerated in growth phase-derived oocytes, as shown by significantly higher (p ≤ 0.01) proportions of both normally fertilized and cleaved oocytes (68.8 and 25.1%), in comparison with dominant phase-derived oocytes (44.2 and 10.3%). In the growth-phase derived oocytes, proportions of normally fertilized and cleaved oocytes were significantly higher (p ≤ 0.01) in oocytes from large (100.0 and 36.4%) and medium (83.3 and 36.5%) follicles than in those from small (54.8 and 14.6%) follicles. The dominant phase-derived oocytes showed higher proportions of normally fertilized and cleaved oocytes in the populations recovered from small (51.5 and 10.0%) and medium (43.1 and 12.0%) follicles than in those from large (25.0 and 0%) follicles; however, the differences were not significant. It can be concluded that: (i) efficiency and kinetics of fertilization differ in relation to oocyte's meiotic competence; (ii) improved development of embryos from oocytes with greater meiotic competence is associated with a more effective fertilization process.
- Type
- Research Article
- Information
- Copyright
- Copyright © Cambridge University Press 2007
References
Alm, H., Torner, H., Lohrke, B., Viergutz, T., Ghoneim, I.M. & Kanitz, W. (2005). Bovine blastocyst development rate in vitro is influenced by selection of oocytes by brillant cresyl blue staining before IVM as indicator for glucose-6-phosphate dehydrogenase activity. Theriogenology 63, 2194–205.Google Scholar
Arlotto, T., Schwartz, J.L., First, N.L. & Leibfried-Rutledge, M.L. (1996). Aspects of follicle and oocyte stage that affect in vitro maturation and development of bovine oocytes. Theriogenology 45, 943–56.CrossRefGoogle ScholarPubMed
de Wit, A.A.C., Wurth, Y.A. & Kruip, T.A.M. (2000). Effect of ovarian phase and follicle quality on morphology and developmental capacity of the bovine cumulus–oocyte complex. J. Anim. Sci. 78, 1277–83.CrossRefGoogle ScholarPubMed
Dominko, T. & First, N.L. (1992). Kinetics of bovine oocyte maturation allows selection for developmental competence and is affected by gonadotropins. Theriogenology 37, 203 abstract.Google Scholar
Eid, L.N., Lorton, S.P. & Parrish, J.J. (1994). Paternal influence on S-phase in the first cell cycle of the bovine embryo. Biol. Reprod. 51, 1232–7.Google Scholar
Farin, P.W. & Farin, C.E. (1995). Transfer of bovine embryos produced in vivo or in vitro: survival and fetal development. Biol. Reprod. 52, 676–82.Google Scholar
Gandolfi, T.A.L. & Gandolfi, F. (2001). The maternal legacy to the embryo: cytoplasmic components and their effects on early development. Theriogenology 55, 1255–76.Google Scholar
Ginther, O.J., Wiltbank, M.C., Fricke, P.M., Gibbons, J.R. & Kot, K. (1996). Selection of the dominant follicle in cattle. Biol. Reprod. 55, 1187–94.Google Scholar
Hagemann, L.J. (1999). Influence of the dominant follicle on oocytes from subordinate follicles. Theriogenology 51, 449–59.CrossRefGoogle ScholarPubMed
Hagemann, L.J., Beaumont, S.E., Berg, M., Donnison, M.J., Ledgard, A., Peterson, A.J., Schurmann, A. & Tervit, H.R. (1999). Development during single IVP of bovine oocytes from dissected follicles: interactive effects of estrous cycle stage, follicle size and atresia. Mol. Reprod. Dev. 53, 451–8.Google Scholar
Hendriksen, P.J.M., Vos, P.L.a.m., Steenweg, W.N.M., Bevers, M.M. & Dieleman, S.J. (2000). Bovine follicular development and its effect on the in vitro competence of oocytes. Theriogenology 53, 11–20.Google Scholar
Hyttel, P., Fair, T., Callesen, H. & Greve, T. (1997). Oocyte growth, capacitation and final maturation in cattle. Theriogenology 47, 23–32.Google Scholar
Iwata, H., Hashimoto, S., Ohota, M., Kimura, K., Shibano, K. & Miyake, M. (2004). Effects of follicle size and electrolytes and glucose in maturation medium on nuclear maturation and developmental competence of bovine oocytes. Reproduction 127, 159–64.Google Scholar
Leibfried-Rutledge, M.L. (1999). Factors determining competence of in vitro produced cattle embryos. Theriogenology 51, 473–85.Google Scholar
Lequarre, A.S., Vigneron, C., Ribaucour, F., Holm, P., Donnay, I., Dalbies-Tran, R., Callesen, H. & Mermillod, P. (2005). Influence of antral follicle size on oocyte characteristics and embryo development in the bovine. Theriogenology 63, 841–59.Google Scholar
Lonergan, P., Monaghan, P., Rizos, D., Boland, M.P. & Gordon, I. (1994). Effect of follicle size on bovine oocyte quality and developmental competence following maturation, fertilization and culture in vitro. Mol. Reprod. Dev. 37, 48–53.Google Scholar
Lonergan, P., Fair, T., Corcoran, D. & Evans, A.C.O. (2006). Effect of culture environment on gene expression and developmental characteristics in IVF-derived embryos. Theriogenology 65, 137–52.Google Scholar
Machatkova, M., Jokesova, E., Horky, F. & Krepelova, A. (2000). Utilization of the growth phase of the first follicular wave for bovine oocyte collection improves blastocyst production. Theriogenology 54, 543–50.CrossRefGoogle ScholarPubMed
Machatkova, M., Krausova, K., Jokesova, E. & Tomanek, M. (2004). Developmental competence of bovine oocytes: effects of follicle size and the phase of follicular wave on in vitro embryo production. Theriogenology 61, 329–35.Google Scholar
Machatkova, M., Hanzalova, K., Horakova, J., Reckova, Z. & Hulinska, P. (2006). Collection of oocytes from donors in the growth phase of follicular development can enhance the production of bovine embryos for cryopreservation. Vet Med-Czech 51, 232–8.Google Scholar
Mihm, M. & Austin, E.J. (2002). The final stages of dominant follicle selection in cattle. Domest. Anim. Endocrinol. 23, 155–66.Google Scholar
Mihm, M., Crowe, M.A., Knight, P.G. & Austin, E.J. (2002). Follicle wave growth in cattle. Reprod. Dom. Anim. 37, 191–200.Google Scholar
Pavlok, A., Lucas-Hahn, A. & Niemann, H. (1992). Fertilization and developmental competence of bovine oocytes derived from different categories of antral follicles. Mol Reprod. Dev. 31, 63–7.Google Scholar
Sirard, M.A. (2001). Resumption of meiosis: mechanism involved in meiotic progression and its relation with developmental competence. Theriogenology 55, 1241–54.Google Scholar
Sirard, M.A., Richard, F., Blondin, P. & Robert, C. (2006). Contribution of the oocyte to the embryo quality. Theriogenology 65, 126–36.Google Scholar
Varisanga, M.D., Sumantri, C., Murakami, M., Fahrudin, M. & Suzuki, T. (1998). Morphological classification of the ovaries in relation to the subsequent oocyte quality for IVF-produced bovine embryos. Theriogenology 50, 1015–23.Google Scholar
Ward, F., Enright, B., Rizos, D., Boland, M. & Lonergan, P. (2002). Optimalization of in vitro bovine embryo production: effect of duration of maturation, lenght of gamete co-incubation, sperm concetration and sire. Theriogenology 57, 2105–17.Google Scholar