Hostname: page-component-78c5997874-ndw9j Total loading time: 0 Render date: 2024-11-05T00:49:13.771Z Has data issue: false hasContentIssue false
  • English
  • Français

Effects of oviductal fluid and heparin on fertility and characteristics of porcine spermatozoa

Published online by Cambridge University Press:  26 September 2008

Nam-Hyung Kim ,
Billy N. Day ,
Joon-Gyo Lim ,
Hoon Taek Lee  and
Kil Saeng Chung
Nam-Hyung Kim
Affiliation:
Animal Resource Research Center, Department of Animal Science, Kon Kuk University, Seoul 143–701, Korea.
Billy N. Day
Affiliation:
Department of Animal Sciences, University of Missouri-Columbia, Columbia, MO 65211, USA.
Joon-Gyo Lim
Affiliation:
Animal Resource Research Center, Department of Animal Science, Kon Kuk University, Seoul 143–701, Korea.
Hoon Taek Lee
Affiliation:
Animal Resource Research Center, Department of Animal Science, Kon Kuk University, Seoul 143–701, Korea.
Kil Saeng Chung*
Affiliation:
Animal Resource Research Center, Department of Animal Science, Kon Kuk University, Seoul 143–701, Korea.
*
Dr Kil-Saeng Chung, Department of Animal Sciences, Kon-Kuk University, Kwangjin Gu, Mojin-dong, Seoul 133-701, Korea. Fax: 822-455-5305.
Get access Rights & Permissions [Opens in a new window]

Summary

The objective of this study was to determine the effects of oviductal fluid and heparin on sperm penetration and the characteristics of spermatozoa. The addition of oviductal fluid and heparin to the fertilisation medium decreased sperm penetration and the mean number of spermatozoa in penetrated eggs. The number of spermatozoa firmly bound to zona pellucida was also decreased in the presence of oviductal fluid and heparin. Chlortetracycline (CTC) fluorescence patterns were used to determine the incidence of capacitation and the acrosome reaction. The proportion of capacitated and acrosome-free spermatozoa increased when spermatozoa were exposed for 1.5 and 3 h to oviductal fluid and heparin. In contrast heparin alone did not increase the number of capacitated spermatozoa at these time points. These results suggest that factor(s) in oviductal secretions reduce polyspermic fertilisation and the number of spermatozoa that will penetrate porcine oocytes. The reduction of polyspermic penetration by oviductal secretions may be due to a reduced number of spermatozoa in the fertilisation medium with an intact acrosome.

Keywords

Acrosome reactionIn vitro fertilisationOviductal fluidPigPolyspermy
Type
Article
Information
Zygote , Volume 5 , Issue 1 , February 1997 , pp. 61 - 65
Copyright
Copyright © Cambridge University Press 1997

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Archibong, A.E., Petters, R.M. & Johnson, B.H.. (1989). Development of porcine embryos from one- and two-cell stages to blastocysts in culture medium supplemented with porcine oviductal fluid. Biol Reprod. 41, 1076–83.CrossRefGoogle ScholarPubMed
DasGupta, S., Mills, C.L. & Fraser, L.R.. (1993). Ca2+-related changes in the capacitation state of human spermatozoa assessed by a chlortetracycline fluorescence assay. J. Reprod. Fertil. 99, 135–43.CrossRefGoogle Scholar
Day, B.N. & Polge, C. (1968). Effects of progesterone on fertilization and egg transport in the pig. J. Reprod. Fertil. 17,227–30.CrossRefGoogle ScholarPubMed
Dubuc, A. & Sirard, M.-A. (1995). Effect of coculturing spermatozoa with oviductal cells on the incidence of polyspermy in pig in vitro fertilization. Mol. Reprod. Dev. 41, 360–7.CrossRefGoogle ScholarPubMed
Fraser, L.R., Abeydeera, L.R. & Niwa, K. (1995). Ca2+ regulating mechanism that modulates bull sperm capacitation and acrosomal exocytosis as determined by chlorotetracycline analysis. Mol. Reprod. Dev. 40, 233–41.CrossRefGoogle ScholarPubMed
Funahashi, H. & Day, B.N. (1993). Effects of follicular fluid at fertilization in vitro on sperm penetration in pig oocytes. J. Reprod. Fertil 99, 97–103.CrossRefGoogle ScholarPubMed
Huang, T.T.F., Flemming, A.D. & Yanagimachi, R. (1981). Only acrosome-reacted spermatozoa can bind and penetrate into zona pellucida: a study using guinea pig. J. Reprod. Dev. 217, 286–90.Google ScholarPubMed
Hunter, R.H.F. (1991). Oviduct function in pigs, with particular reference to the pathological condition of polyspermy. Mol. Reprod. Dev. 29, 385–91.CrossRefGoogle Scholar
Hunter, R.H.F. (1994). Modulation of gamete and embryonic microenvironments by oviduct glycoprotein. Mol. Reprod. Dev. 39, 176–81.CrossRefGoogle Scholar
Jones, R. (1991). Interaction of zona pellucida glycoproteins, sulfated carbohydrates and synthetic polymers with proacrosin, the putative egg-binding protein from mammalian spermatozoa. Development 111, 1155–63.CrossRefGoogle ScholarPubMed
Kim, N.-H., Funahashi, H., Moon, S.J., Abeydeera, L., Prather, R.S. & Day, B.N. (1996a). Effects of oviductal fluid on the cortical granule reaction and polyspermy in the porcine oocytes. J. Reprod. Fertil. 107, 879–86.Google Scholar
Kim, N.-H., Kim, J.H., Lee, H.T. & Chung, K.S. (1996b). Effect of extracellular potassium concentration on the meiotic and cytoplasmic maturation. Anim. Reprod. Sci. 44, 175–82.CrossRefGoogle Scholar
Mattioli, M., Galeati, G. & Seren, E. (1988). Effect of follicle somatic cell during pig oocyte maturation on egg penetrability and male pronuclear formation. Gamete Res. 20, 177–83.CrossRefGoogle Scholar
Nagai, T. & Moor, R.M. (1990). Effect of oviductal cells on the incidence of polyspermy in pig eggs fertilized in vitro. Mol. Reprod. Dev. 26, 377–82.CrossRefGoogle ScholarPubMed
Nagai, T., Niwa, K. & Iritani, A. (1984). Effects of sperm concentration during preincubation in a defined medium on fertilization in vitro of pig follicular oocytes. J. Reprod. Fertil. 70, 271–7.CrossRefGoogle Scholar
Niwa, K. & Ohgoda, O. (1988). Synergistic effect of caffeine and heparin on in vitro fertilization of cattle oocytes matured in culture. Theriogenology 30, 733–41.CrossRefGoogle ScholarPubMed
Parrish, J.J., Susko-Parish, J.L., Winer, M.A. & First, N.L. (1988). Capacitation of bovine spermatozoa by heparin. Biol. Reprod. 38, 1171–80.CrossRefGoogle ScholarPubMed
Parrish, J.J., Susko-Parish, J.L., Handrow, R.R., Sims, M.M. & First, N.L. (1989). Capacitation of bovine spermatozoa by oviductal fluid. Biol. Reprod. 40, 1020–5.CrossRefGoogle Scholar
Petters, R.M. & Wells, K.D. (1993). Culture of pig embryos. J. Reprod. Fertil. (Suppl.) 48, 61–73.Google ScholarPubMed
Schroeder, A.C., Schultz, R.M., Kopf, G.S., Taylor, F.R., Becker, R.B. & Eppig, J.J. (1991). Fetuin inhibits zona pellucida hardening and conversion of ZP2 to ZP2f during spontaneous mouse oocyte maturation in vitro in the absence of serum. Biol. Reprod. 43, 46–55.Google Scholar
Wang, W.H., Niwa, K. & Okuda, K. (1991). In vitro penetration of pig oocytes matured in culture by frozen-thawed ejaculated spermatozoa. J. Reprod. Fertil. 93, 491–6.CrossRefGoogle ScholarPubMed
Wang, W.H., Abeydeera, L.R., Fraser, L.R. & Niwa, K. (1995). Functional analysis using chlortetracycline fluorescence and in vitro fertilization of frozen-thawed ejaculated boar spermatozoa incubated in a protein free chemically defined medium. J. Reprod. Fertil. 104, 305–13.CrossRefGoogle Scholar
Ward, C.R. & Storey, B.T. (1984). Determination of the time course of capacitation in mouse spermatozoa using a chlortetracycline fluorescence assay. Dev. Biol. 104, 287–96.CrossRefGoogle ScholarPubMed
Wassarman, P.M. (1988). Zona pellucida glycoproteins. Annu. Rev. Biochem. 57, 415–42.CrossRefGoogle ScholarPubMed