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The effects of methyl-β-cyclodextrin on in vitro fertilization and the subsequent development of bovine oocytes

Published online by Cambridge University Press:  24 March 2010

Yoshikazu Nagao ,
Yuki Ohta ,
Hidemi Murakami  and
Yoku Kato
Yoshikazu Nagao*
Affiliation:
University Farm, Faculty of Agriculture, Utsunomiya University, 443 Shimokomoriya, Mohka, Tochigi 321–4415, Japan. Department of Animal Production Science, United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Tokyo, Japan.
Yuki Ohta
Affiliation:
University Farm, Department of Animal Science, Faculty of Agriculture, Utsunomiya University, Tochigi, Japan. Science Service Inc., Japan.
Hidemi Murakami
Affiliation:
University Farm, Department of Animal Science, Faculty of Agriculture, Utsunomiya University, Tochigi, Japan.
Yoku Kato
Affiliation:
Department of Animal Production Science, United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Tokyo, Japan.
*
All correspondence to: Yoshikazu Nagao. University Farm, Faculty of Agriculture, Utsunomiya University, 443 Shimokomoriya, Mohka, Tochigi 321–4415, Japan. Tel: +81 285 84 1321. Fax: +81 285 84 1321. e-mail: [email protected]
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Summary

In this study, we investigated the effects of methyl-β-cyclodextrin (MBCD) on in vitro fertilization and the subsequent development of bovine oocytes. Bovine oocytes matured in serum-free medium were inseminated with frozen–thawed sperm pre-incubated in protein-free modified Brackett and Oliphant medium (BO) containing various concentrations of MBCD for various periods. MBCD decreased the frequency of live sperm, however enhanced the capacitation and acrosome reaction of the live sperm. Pre-incubation of sperm with 0.5, 1.0 and 1.5 mM MBCD for 2 and 4 h increased the frequency of normal fertilization. Embryos derived from oocytes fertilized with spermatozoa pre-incubated with MBCD developed normally to the blastocyst stage and term. There were individual differences and similar tendencies in four different sires in terms of the effects of MBCD upon fertilization. These results indicate that the pre-incubation of bovine sperm with MBCD affects viability and capacitation status of the sperm and promotes fertilization in vitro. Embryos derived from oocytes fertilized with sperm pre-incubated with MBCD developed normally to the blastocyst stage and term.

Keywords

BovineDevelopmentFertilizationMethyl-β-cyclodextrinOocyte
Type
Research Article
Information
Zygote , Volume 18 , Issue 4 , November 2010 , pp. 323 - 330
Copyright
Copyright © Cambridge University Press 2010

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References

Austin, C.R. (1951). Observations on the penetration of the sperm into the mammalian egg. Aust. J. Sci. Res. (B) 4, 581–96.Google ScholarPubMed
Brackett, B.G. & Oliphant, G. (1975). Capacitation of rabbit spermatozoa in vitro. Biol. Reprod. 12, 260–74.Google Scholar
Chang, M.C. (1951). Fertilizing capacity of spermatozoa deposited into fallopian tubes. Nature 168, 697–8.Google Scholar
Chapman, D. (1982). Protein–lipid interactions in model and natural biomembranes. In Biological Membranes (ed. Chapman, D.), pp. 177–224. New York: Academic Press.Google Scholar
Choi, Y.H. & Toyoda, Y. (1998). Cyclodextrin removes cholesterol from mouse sperm and induces capacitation in a protein-free medium. Biol. Reprod. 59, 1328–33.Google Scholar
Davis, B.K. (1978). Inhibition of fertilizing capacity in mammalian spermatozoa by natural and synthetic vesicles. In Symposium on the Pharmacological Effects of Lipids (ed. Kabara, J.J.), pp. 145–57. Am. Oil Chem. Soc.Google Scholar
Davis, B.K. (1981). Timing of fertilization in mammals: sperm cholesterol/phospholipids ratio as a determinant of the capacitation interval. Proc. Natl. Acad. Sci. 78, 7560–4.Google Scholar
Dinkins, M.B. & Brackett, B.G. (2000). Chlortetracycline staining patterns of frozen–thawed bull spermatozoa treated with beta-cyclodextrins, dibutyryl cAMP and progesterone. Zygote 8, 245–56.CrossRefGoogle Scholar
Ehrenwald, E., Parks, J.E. & Foote, R.H. (1988a). Cholesterol efflux from bovine sperm. Induction of the acrosome reaction with lysophosphatidylcholine after reducing sperm cholesterol. Gamete Res. 20, 145–57.CrossRefGoogle ScholarPubMed
Ehrenwald, E., Parks, J.E. & Foote, R.H. (1988b). Cholesterol efflux from bovine sperm. Effect of reducing sperm cholesterol on penetration of zona-free hamster and in vitro matured bovine ova. Gamete Res. 20, 413–420.Google Scholar
Fraser, L.R., Abeydeera, L.R. & Niwa, K. (1995). Ca2+-regulating mechanisms that modulate bull sperm capacitation and acrosomal exocytosis as determined by chlortetracycline analysis. Mol. Reprod. Dev. 40, 233–41.Google Scholar
Hoshi, K., Aita, T., Yanagida, K., Yoshimatu, N. & Sato, A. (1990). Variation in the cholesterol/phospholipid ratio in human spermatozoa and its relationship with capacitation. Hum. Reprod. 5, 71–4.CrossRefGoogle ScholarPubMed
Irie, T., Fukunaga, K. & Pitha, J. (1992). Hydroxypropylcyclodextrins in parenteral use: lipid dissolution and effects on lipid transfers in vitro. J. Pharmacol. Sci. 81, 521–3.Google Scholar
Irie, T., Otagiri, M., Sunada, M., Uekama, K., Ohtani, Y., Yamada, Y. & Sugiyama, Y. (1982). Cyclodextrin-induced hemolysis and shape changes of human erythrocytes in vitro. J. Pharm. Dyn. 5, 741–4.CrossRefGoogle ScholarPubMed
Kilsdonk, E.P.C., Yancey, P.C., Stoudt, G.W., Bangerter, F.W., Johnson, W.J., Phillips, M.C. & Rothblat, G.H. (1995). Cellular cholesterol efflux mediated by cyclodextrins. J. Biol. Chem. 270, 17250–6.CrossRefGoogle ScholarPubMed
Maurer, H.R. (1992). Towards serum-free, chemically defined media for mammalian cell culture. In Animal Cell Culture: A Practical Approach (ed. Freshney, R.I.), pp. 15–46. Oxford: Oxford University Press.Google Scholar
Nagao, Y., Iijima, R. & Saeki, K. (2008). Interaction between embryo and culture condition during in in vitro development of bovine early embryos. Zygote 16, 127–33.CrossRefGoogle ScholarPubMed
Saeki, K., Nagao, Y., Hoshi, M. & Nagai, M. (1995). Effects of heparin, sperm concentration and bull variation on in vitro fertilization of bovine oocytes in a protein-free medium. Theriogenology 43, 751–9.CrossRefGoogle Scholar
Stern, W.C. (1989). Cyclodextrin-based drug delivery. Drug News Perspect. 2, 410–5.Google Scholar
Szejtli, J. (1988). Cyclodextrin Technology. Dordrecht, The Netherlands: Kluwer Academic Publishers.Google Scholar
Takeo, T., Hoshii, T., Kondo, Y., Toyodome, H., Arima, H., Yamamura, K., Irie, T. & Nakagata, N. (2008). Methyl-beta-cyclodextrin improves fertilizing ability of C57BL/6 mouse sperm after freezing and thawing by facilitating cholesterol efflux from the cells. Biol. Reprod. 78, 546–51.Google Scholar
Yanagimachi, R. (1994). Mammalian fertilization in. In Physiology of Reproduction (eds Knobil, E. & Neill, J.D.), pp. 189–318. New York: Raven Press.Google Scholar
Yeagle, P.L. (1985). Cholesterol and the cell membrane. Biochim. Biophys. Acta, 882, 267–87.Google Scholar
Yancey, PG., Rodriguez, W.V., Kilsdonk, E.P., Stoudt, G.W., Johnson, W.J., Phillips, M.C. & Rothblat, G.H. (1996). Cellular cholesterol efflux mediated by cyclodextrins. J. Biol. Chem. 271, 16026–34.CrossRefGoogle ScholarPubMed
Yoshizawa, M., Konno, H., Zhu, S., Kagegama, S., Fukui, E., Muramatsu, S., Kim, S. & Araki, Y. (1999). Chromosomal diagnosis in each individual blastomere of 5- to 10-cell bovine embryos derived from in vitro fertilization. Theriogenology 51, 1239–50.Google Scholar