Hostname: page-component-cd9895bd7-dzt6s Total loading time: 0 Render date: 2024-12-24T16:33:54.512Z Has data issue: false hasContentIssue false
  • English
  • Français

Effects of high calcium levels on the disturbed extrusion of the second polar body during in vitro fertilization in C3H/He mouse substrains

Published online by Cambridge University Press:  29 October 2019

Y. Ohta-Takada Open the ORCID record for Y. Ohta-Takada [Opens in a new window] ,
Y. Nagao  and
S. Kito
Y. Ohta-Takada
Affiliation:
Science Service Inc., Chiba, Japan University Farm, Faculty of Agriculture, Utsunomiya University, Tochigi, Japan Department of Technical Support and Development, National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology, Chiba, Japan
Y. Nagao
Affiliation:
University Farm, Faculty of Agriculture, Utsunomiya University, Tochigi, Japan
S. Kito*
Affiliation:
Department of Technical Support and Development, National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology, Chiba, Japan
*
Author for correspondence: Seiji Kito, Centre of Animal Research and Education, Nagoya University, Furo-cho Chikusa, Nagoya, 464–8601, Japan. Tel: +81 52 747 6875. Fax: +81 52 747 6874. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Summary

We previously reported that high concentrations (≥3.42 mM) of calcium during in vitro fertilization (IVF) disturbed the extrusion of the second polar body (PBII) in C3H/He inbred mice. In this study, the substrain specificity of this phenomenon was examined under 1.71–6.84 mM calcium concentration in ova from six C3H/He mouse commercially available substrains in Japan. PBII extrusion in ova from J substrains was not affected by calcium concentrations (<10% at any calcium level), but was grossly disturbed at high calcium levels in the ova of other substrains. This result has practical applications for the efficient production of normal zygotes by IVF, therefore contributing to the reduction in the numbers of donor animals for further zygote or embryo manipulation. Care must be taken in choosing IVF medium for particular strains and substrains.

Keywords

C3H/HeCalciumIn vitro fertilizationReproductionSecond polar body
Type
Short Communication
Information
Zygote , Volume 28 , Issue 1 , February 2020 , pp. 83 - 85
Copyright
© Cambridge University Press 2019 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

*

Present address: Yakult Central Institute, Tokyo, Japan.

†

Present address: Centre of Animal Research and Education, Nagoya University, Nagoya, Japan.

References

Byers, SL, Payson, SJ and Taft, RA (2006) Performance of ten inbred mouse strains following assisted reproductive technologies (ARTs). Theriogenology 65, 1716–26.CrossRefGoogle Scholar
Festing, MF and Blackmore, DK (1971) Life span of specified-pathogen-free (MRC category 4) mice and rats. Lab Anim 5, 179–92.CrossRefGoogle ScholarPubMed
Kito, S and Ohta, Y (2008) In vitro fertilization in inbred BALB/c mice I: isotonic osmolarity and increased calcium-enhanced sperm penetration through the zona pellucida and male pronuclear formation. Zygote 16, 249–57.CrossRefGoogle ScholarPubMed
Liu, L, Nutter, LM, Law, N and McKerlie, C (2009) Sperm freezing and in vitro fertilization in three substrains of C57BL/6 mice. J Am Assoc Lab Anim Sci 48, 39–43.Google ScholarPubMed
Ohta, Y, Nagao, Y, Minami, N, Tsukamoto, S and Kito, S (2016) Deficiencies in extrusion of the second polar body due to high calcium concentrations during in vitro fertilization in inbred C3H/He mice. Zygote 24, 603–16.CrossRefGoogle ScholarPubMed
Poltorak, A, He, X, Smirnova, I, Liu, MY, Van Huffel, C, Du, X, Birdwell, D, Alejos, E, Silva, M, Galanos, C, Freudenberg, M, Ricciardi-Castagnoli, P, Layton, B and Beutler, B (1998) Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice: mutations in Tlr4 gene. Science 282, 2085–8.CrossRefGoogle ScholarPubMed
Simon, MM, Greenaway, S, White, JK, Fuchs, H, Gailus-Durner, V, Wells, S, Sorg, T, Wong, K, Bedu, E, Cartwright, EJ et al. (2013) A comparative phenotypic and genomic analysis of C57BL/6J and C57BL /6N mouse strains. Genome Biol 14, R82.CrossRefGoogle ScholarPubMed
Thornton, CE, Brown, SD and Glenister, PH (1999) Large numbers of mice established by in vitro fertilization with cryopreserved spermatozoa: implications and applications for genetic resource banks, mutagenesis screens and mouse backcrosses. Mamm Genome 10, 987–92.CrossRefGoogle ScholarPubMed
Vasudevan, K and Sztein, JM (2012) In vitro fertility rate of 129 strains is improved by buserelin (gonadotropin-releasing hormone) administration prior to superovulation. Lab Anim 46, 299–303.CrossRefGoogle ScholarPubMed
Whitmore, AC and Whitmore, SP (1985) Subline divergence within LC Strong’s C3H and CBA inbred mouse strains. A review. Immunogenetics 21, 407–28.CrossRefGoogle Scholar