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Effects of follicular fluids on the growth of porcine preantral follicle and oocyte

Published online by Cambridge University Press:  01 August 2008

T. Metoki ,
H. Iwata ,
M. Itoh ,
M. Kasai ,
A. Takajyo ,
A. Suzuki ,
T. Kuwayama  and
Y. Monji
T. Metoki
Affiliation:
Tokyo University of Agriculture, Department of Animal Science, Funako 1737, Atsugi City, Kanagawa Prefecture 243–0034, Japan.
H. Iwata*
Affiliation:
Tokyo University of Agriculture, Department of Animal Science, Funako 1737, Atsugi City, Kanagawa Prefecture 243–0034, Japan. Tokyo University of Agriculture, Department of Animal Science, Funako 1737, Atsugi City, Kanagawa Prefecture 243–0034, Japan.
M. Itoh
Affiliation:
Tokyo University of Agriculture, Department of Animal Science, Funako 1737, Atsugi City, Kanagawa Prefecture 243–0034, Japan.
M. Kasai
Affiliation:
Tokyo University of Agriculture, Department of Animal Science, Funako 1737, Atsugi City, Kanagawa Prefecture 243–0034, Japan.
A. Takajyo
Affiliation:
Tokyo University of Agriculture, Department of Animal Science, Funako 1737, Atsugi City, Kanagawa Prefecture 243–0034, Japan.
A. Suzuki
Affiliation:
Tokyo University of Agriculture, Department of Animal Science, Funako 1737, Atsugi City, Kanagawa Prefecture 243–0034, Japan.
T. Kuwayama
Affiliation:
Tokyo University of Agriculture, Department of Animal Science, Funako 1737, Atsugi City, Kanagawa Prefecture 243–0034, Japan.
Y. Monji
Affiliation:
Tokyo University of Agriculture, Department of Animal Science, Funako 1737, Atsugi City, Kanagawa Prefecture 243–0034, Japan.
*
All correspondence to H. Iwata, Tokyo University of Agriculture, Department of Animal Science, Funako 1737, Atsugi City, Kanagawa Prefecture 243–0034, Japan. Tel: +81 46 270 6587. Fax: +81 46 270 6587. e-mail: [email protected]
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Summary

We examined the effect of supplementing the culture medium with follicular fluid (FF) on the growth of porcine preantral follicles and oocytes. Firstly, preantral follicles were retrieved from ovaries and then FF was collected from all antral follicles that were 2–7 mm in diameter (AFF), which included large follicles of 4–7 mm in diameter (LFF) and small follicles of 2–3 mm in diameter (SFF). When preantral follicles with a diameter of 250 μm were cultured in medium containing AFF, the growth of follicles and oocytes was greater than when follicles were cultured in medium containing fetal calf serum (FCS). When this growth-promoting effect in AFF was compared for LFF and SFF, the LFF were shown to be significantly more effective than SFF. This LFF effect was lost, however, when the concentration of LFF in the medium was decreased from 5% to 0.5% or when LFF were heat treated (60 °C for 30 min) or trypsin was added. In contrast, a decrease in SFF concentration from 5% to 0.5% and heat treatment of the SFF enhanced preantral follicle growth. Furthermore, proteins obtained from LFF that had molecular weights greater than 10 kDa (LFF > 10 kDa) had similar, but relatively reduced, growth-promoting properties. The remaining three LFF protein fractions (<10 kDa or <100 kDa or >100 kDa), however, did not have these growth-promoting properties. In conclusion, the supplementation of medium with LFF, rather than serum, enhanced preantral follicle and oocyte growth. Factors that enhanced follicle development in LFF and factors that suppressed follicle development in SFF were proteins and these LFF factors ranged in size from 10 kDa to over 100 kDa.

Keywords

DiameterFollicular fluidOocytePigPreantral follicle
Type
Research Article
Information
Zygote , Volume 16 , Issue 3 , August 2008 , pp. 239 - 247
Copyright
Copyright © Cambridge University Press 2008

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