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Effects of different polyvinylpyrrolidone concentrations on intracytoplasmic sperm injection

Published online by Cambridge University Press:  14 January 2020

Ding Ding ,
Qiushuang Wang ,
Xinyuan Li ,
Beili Chen ,
Weiwei Zou ,
Dongmei Ji ,
Yan Hao ,
Rufeng Xue ,
Huijuan Zou  and
Zhaolian Wei
...Show all authors
Ding Ding
Affiliation:
Reproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, No. 218 Jixi Road, Hefei230022, Anhui, China NHC Key Laboratory of study on abnormal gametes and reproductive tract (Anhui Medical University), No. 81 Meishan Road, Hefei230032, Anhui, China Key Laboratory of Population Health Across Life Cycle (Anhui Medical University), Ministry of Education of the People’s Republic of China, No. 81 Meishan Road, Hefei230032, Anhui, China
Qiushuang Wang
Affiliation:
Reproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, No. 218 Jixi Road, Hefei230022, Anhui, China NHC Key Laboratory of study on abnormal gametes and reproductive tract (Anhui Medical University), No. 81 Meishan Road, Hefei230032, Anhui, China Key Laboratory of Population Health Across Life Cycle (Anhui Medical University), Ministry of Education of the People’s Republic of China, No. 81 Meishan Road, Hefei230032, Anhui, China
Xinyuan Li
Affiliation:
Reproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, No. 218 Jixi Road, Hefei230022, Anhui, China Anhui Province Key Laboratory of Reproductive Health and Genetics, No. 81 Meishan Road, Hefei230032, Anhui, China Biopreservation and Artificial Organs, Anhui Provincial Engineering Research Center, Anhui Medical University, No. 81 Meishan Road, Hefei230032, Anhui, China
Beili Chen
Affiliation:
Reproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, No. 218 Jixi Road, Hefei230022, Anhui, China Anhui Province Key Laboratory of Reproductive Health and Genetics, No. 81 Meishan Road, Hefei230032, Anhui, China Biopreservation and Artificial Organs, Anhui Provincial Engineering Research Center, Anhui Medical University, No. 81 Meishan Road, Hefei230032, Anhui, China
Weiwei Zou
Affiliation:
Reproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, No. 218 Jixi Road, Hefei230022, Anhui, China Anhui Province Key Laboratory of Reproductive Health and Genetics, No. 81 Meishan Road, Hefei230032, Anhui, China Biopreservation and Artificial Organs, Anhui Provincial Engineering Research Center, Anhui Medical University, No. 81 Meishan Road, Hefei230032, Anhui, China
Dongmei Ji
Affiliation:
Reproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, No. 218 Jixi Road, Hefei230022, Anhui, China Anhui Province Key Laboratory of Reproductive Health and Genetics, No. 81 Meishan Road, Hefei230032, Anhui, China Biopreservation and Artificial Organs, Anhui Provincial Engineering Research Center, Anhui Medical University, No. 81 Meishan Road, Hefei230032, Anhui, China
Yan Hao
Affiliation:
Reproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, No. 218 Jixi Road, Hefei230022, Anhui, China Anhui Province Key Laboratory of Reproductive Health and Genetics, No. 81 Meishan Road, Hefei230032, Anhui, China Biopreservation and Artificial Organs, Anhui Provincial Engineering Research Center, Anhui Medical University, No. 81 Meishan Road, Hefei230032, Anhui, China
Rufeng Xue
Affiliation:
Reproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, No. 218 Jixi Road, Hefei230022, Anhui, China Anhui Province Key Laboratory of Reproductive Health and Genetics, No. 81 Meishan Road, Hefei230032, Anhui, China Biopreservation and Artificial Organs, Anhui Provincial Engineering Research Center, Anhui Medical University, No. 81 Meishan Road, Hefei230032, Anhui, China
Huijuan Zou
Affiliation:
Reproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, No. 218 Jixi Road, Hefei230022, Anhui, China Anhui Province Key Laboratory of Reproductive Health and Genetics, No. 81 Meishan Road, Hefei230032, Anhui, China Biopreservation and Artificial Organs, Anhui Provincial Engineering Research Center, Anhui Medical University, No. 81 Meishan Road, Hefei230032, Anhui, China
Zhaolian Wei
Affiliation:
Reproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, No. 218 Jixi Road, Hefei230022, Anhui, China Anhui Province Key Laboratory of Reproductive Health and Genetics, No. 81 Meishan Road, Hefei230032, Anhui, China Biopreservation and Artificial Organs, Anhui Provincial Engineering Research Center, Anhui Medical University, No. 81 Meishan Road, Hefei230032, Anhui, China
Ping Zhou
Affiliation:
Reproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, No. 218 Jixi Road, Hefei230022, Anhui, China Anhui Province Key Laboratory of Reproductive Health and Genetics, No. 81 Meishan Road, Hefei230032, Anhui, China Biopreservation and Artificial Organs, Anhui Provincial Engineering Research Center, Anhui Medical University, No. 81 Meishan Road, Hefei230032, Anhui, China
Yunxia Cao
Affiliation:
Reproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, No. 218 Jixi Road, Hefei230022, Anhui, China Anhui Province Key Laboratory of Reproductive Health and Genetics, No. 81 Meishan Road, Hefei230032, Anhui, China Biopreservation and Artificial Organs, Anhui Provincial Engineering Research Center, Anhui Medical University, No. 81 Meishan Road, Hefei230032, Anhui, China
Zhiguo Zhang*
Affiliation:
Reproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, No. 218 Jixi Road, Hefei230022, Anhui, China NHC Key Laboratory of study on abnormal gametes and reproductive tract (Anhui Medical University), No. 81 Meishan Road, Hefei230032, Anhui, China Key Laboratory of Population Health Across Life Cycle (Anhui Medical University), Ministry of Education of the People’s Republic of China, No. 81 Meishan Road, Hefei230032, Anhui, China
*
Author for correspondence: Zhiguo Zhang. Reproductive Medicine Center, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, Hefei230022, China. Tel:/Fax: +86 181 5510 6683. E-mail: [email protected]
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Summary

To explore whether different polyvinylpyrrolidone (PVP) concentrations affect the results of intracytoplasmic sperm injection (ICSI), a prospective study was conducted for 194 couples undergoing 210 ICSI therapy cycles. These cycles were divided into three groups (10, 7 and 5% groups) using the corresponding concentration of PVP for sperm immobilization. The main outcome measures were analyzed. Results indicated that, with a decrease in PVP concentrations, all of the main outcome measures increased. In particular, the high-quality cleavage embryo rate in the 7% group was significantly lower than in the 5% group (P < 0.01), and the cleavage, high-quality cleavage embryo, and high-quality blastocyst rates in the 5% group were significantly higher than those in the 10% group (all P < 0.001). For high-/intermediate-quality semen, all of the main outcome measures were significantly increased with 5% PVP. For the poor-quality semen, only the high-quality cleavage embryo and high-quality blastocyst rates were significantly higher in the 5% group. Therefore, lowering PVP concentrations greatly promoted the development of embryos in ICSI cycles, with an optimal concentration of 5% for ICSI.

Keywords

Polyvinylpyrrolidoneconcentrationintracytoplasmic sperm injectionsemendevelopment
Type
Research Article
Information
Zygote , Volume 28 , Issue 2 , April 2020 , pp. 148 - 153
Copyright
© Cambridge University Press 2020

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Footnotes

*

These authors contributed equally to this work.

References

Andersen, AN, Goossens, V, Ferraretti, AP, Bhattacharya, S, Felberbaum, R, de Mouzon, J and Nygren, KG (2008) Assisted reproductive technology in Europe, 2004: results generated from European registers by ESHRE. Hum Reprod 23, 756–71.CrossRefGoogle ScholarPubMed
Damsgaard, J, Joensen, UN, Carlsen, E, Erenpreiss, J, Jensen, MB, Matulevicius, V, Zilaitiene, B, Olesen, IA, Perheentupa, A, Punab, M, Salzbrunn, A, Toppari, J, Virtanen, HE, Juul, A, Skakkebaek, NE and Jorgensen, N (2017) Reply to Eugenio Ventimiglia, Francesco Montorsi, and Andrea Salonia’s Letter to the Editor re: Jakob Damsgaard, Ulla N. Joensen, Elisabeth Carlsen, et al. (2016) Varicocele is associated with impaired semen quality and reproductive hormone levels: a study of 7035 healthy young men from six European countries. Eur Urol 70, 1019–29. Eur Urol 71, e71–2.CrossRefGoogle Scholar
Esteves, SC and Varghese, AC (2012) Laboratory handling of epididymal and testicular spermatozoa: What can be done to improve sperm injections outcome. J Hum Reprod Sci 5, 233–43.CrossRefGoogle ScholarPubMed
Esteves, SC, Roque, M, Bradley, CK and Garrido, N (2017) Reproductive outcomes of testicular versus ejaculated sperm for intracytoplasmic sperm injection among men with high levels of DNA fragmentation in semen: systematic review and meta-analysis. Fertil Steril 108, 456–67.e1.CrossRefGoogle ScholarPubMed
Ford, WC (2010) Comments on the release of the 5th edition of the WHO Laboratory Manual for the Examination and Processing of Human Semen. Asian J Androl 12, 59–63.CrossRefGoogle ScholarPubMed
Gardner, DK, Lane, M, Stevens, J, Schlenker, T and Schoolcraft, WB (2000) Blastocyst score affects implantation and pregnancy outcome: towards a single blastocyst transfer. Fertil Steril 73, 1155–8.CrossRefGoogle ScholarPubMed
Gerris, J, Mangelschots, K, Van Royen, E, Joostens, M, Eestermans, W and Ryckaert, G (1995) ICSI and severe male-factor infertility: breaking the sperm tail prior to injection. Hum Reprod 10, 484–6.CrossRefGoogle ScholarPubMed
Hernandez-Lopez, L, Umland, N, Mondragon-Ceballos, R and Nayudu, PL (2005) Comparison of the effects of Percoll and PureSperm on the common marmoset (Callithrix jacchus) semen. J Med Primatol 34, 86–90.CrossRefGoogle ScholarPubMed
Hlinka, D, Herman, M, Vesela, J, Hredzak, R, Horvath, S and Pacin, J (1998) A modified method of intracytoplasmic sperm injection without the use of polyvinylpyrrolidone. Hum Reprod 13, 1922–7.CrossRefGoogle ScholarPubMed
Hussain, T, Ranjha, NM and Shahzad, Y (2011) Swelling and controlled release of tramadol hydrochloride from a pH-sensitive hydrogel. Des Monomers Polym 14, 233–49.CrossRefGoogle Scholar
Kato, Y and Nagao, Y (2009) Effect of PVP on sperm capacitation status and embryonic development in cattle. Theriogenology 72, 624–35.CrossRefGoogle ScholarPubMed
Kato, Y and Nagao, Y (2012) Effect of polyvinylpyrrolidone on sperm function and early embryonic development following intracytoplasmic sperm injection in human assisted reproduction. Reprod Med Biol 11, 165–76.CrossRefGoogle ScholarPubMed
Kimura, Y and Yanagimachi, R (1995) Intracytoplasmic sperm injection in the mouse. Biol Reprod 52, 709–20.CrossRefGoogle ScholarPubMed
Li, Z, Wang, AY, Bowman, M, Hammarberg, K, Farquhar, C, Johnson, L, Safi, N and Sullivan, EA (2018) ICSI does not increase the cumulative live birth rate in non-male factor infertility. Hum Reprod 33, 1322–30.CrossRefGoogle Scholar
Luna, D, Hilario, R, Duenas-Chacon, J, Romero, R, Zavala, P, Villegas, L and Garcia-Ferreyra, J (2015) The IMSI procedure improves laboratory and clinical outcomes without compromising the aneuploidy rate when compared to the classical ICSI procedure. Clin Med Insights Reprod Health 9, 29–37.CrossRefGoogle ScholarPubMed
Martin, MJ (2000) Development of in vivo-matured porcine oocytes following intracytoplasmic sperm injection. Biol Reprod 63, 109–12.CrossRefGoogle ScholarPubMed
Palermo, GD, Neri, QV, Schlegel, PN and Rosenwaks, Z (2014) Intracytoplasmic sperm injection (ICSI) in extreme cases of male infertility. PLoS One 9, e113671.CrossRefGoogle ScholarPubMed
Rosenwaks, Z. and Pereira, N (2017) The pioneering of intracytoplasmic sperm injection: historical perspectives. Reproduction 154, F71–7.CrossRefGoogle ScholarPubMed
Rubino, P, Vigano, P, Luddi, A and Piomboni, P (2016) The ICSI procedure from past to future: a systematic review of the more controversial aspects. Hum Reprod Update 22, 194–227.Google ScholarPubMed
Schwarzer, JU, Fiedler, K, Hertwig, I, Krusmann, G, Wurfel, W, Muhlen, B, Pickl, U, Lochner-Ernst, D, Schleyer, M, Ovens-Rader, A and Hennig, M (2003) Male factors determining the outcome of intracytoplasmic sperm injection with epididymal and testicular spermatozoa. Andrologia 35, 220–6.CrossRefGoogle ScholarPubMed
Simopoulou, M, Gkoles, L, Bakas, P, Giannelou, P, Kalampokas, T, Pantos, K and Koutsilieris, M (2016) Improving ICSI: a review from the spermatozoon perspective. Syst Biol Reprod Med 62, 359–71.CrossRefGoogle ScholarPubMed
Tomas, C, Orava, M, Tuomivaara, L and Martikainen, H (1998) Low pregnancy rate is achieved in patients treated with intracytoplasmic sperm injection due to previous low or failed fertilization in in-vitro fertilization. Hum Reprod 13, 65–70.CrossRefGoogle ScholarPubMed
Vanden Meerschaut, F, Nikiforaki, D, Heindryckx, B and De Sutter, P (2014) Assisted oocyte activation following ICSI fertilization failure. Reprod Biomed Online 28, 560–71.CrossRefGoogle ScholarPubMed
Velaers, A, Paternot, G, Debrock, S, D’Hooghe, T and Spiessens, C (2012) Triple touch sperm immobilization vs. single touch sperm immobilization in ICSI – a randomised trial. Reprod Biol Endocrinol 10, 65.CrossRefGoogle ScholarPubMed
Wang, YX, Wu, Y, Chen, HG, Duan, P, Wang, L, Shen, HQ, Lu, WQ, Sun, B, Wang, Q, Zhang, B, Chavarro, JE, Zhang, J and Pan, A (2019) Seminal plasma metabolome in relation to semen quality and urinary phthalate metabolites among Chinese adult men. Environ Int 129, 354–63.CrossRefGoogle ScholarPubMed
Ward, MA and Yanagimachi, R (2018) Intracytoplasmic sperm injection in mice. Cold Spring Harb Protoc, 2018.CrossRefGoogle Scholar
World Health Organization (2010) WHO Laboratory Manual for the Examination and Processing of Human Semen, 5th edn. Geneva, Switzerland: WHO Press.Google Scholar
Yeste, M, Jones, C, Amdani, SN and Coward, K (2017) Oocyte activation and fertilisation: crucial contributors from the sperm and oocyte. Results Probl Cell Differ 59, 213–39.CrossRefGoogle ScholarPubMed
Yoeli, R, Orvieto, R, Ashkenazi, J, Shelef, M, Ben-Rafael, Z and Bar-Hava, I (2008) Comparison of embryo quality between intracytoplasmic sperm injection and in vitro fertilization in sibling oocytes. J Assist Reprod Genet 25, 23–8.CrossRefGoogle ScholarPubMed