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The effect of temperature on the initial development of Brycon amazonicus Spix & Agassiz, 1829 as tool for micromanipulation of embryos

Published online by Cambridge University Press:  20 September 2017

Regiane Cristina da Silva ,
Matheus Pereira dos Santos ,
José Augusto Senhorini ,
Maria do Carmo Faria Paes ,
Fernanda Nogueira Valentin ,
Takafumi Fujimoto ,
Nivaldo Ferreira do Nascimento ,
George Shigueki Yasui  and
Laura Satiko Okada Nakaghi
Regiane Cristina da Silva*
Affiliation:
UNESP's Aquaculture Centre (CAUNESP), UNESP – São Paulo State University, Campus Jaboticabal-SP. Via de Acesso Prof. Paulo Donato Castellane s/n, CEP 14884-900, Jaboticabal, SPBrazil.
Matheus Pereira dos Santos
Affiliation:
UNESP's Aquaculture Centre (CAUNESP), UNESP – São Paulo State University, Campus Jaboticabal-SP. Via de Acesso Prof. Paulo Donato Castellane s/n, CEP 14884-900, Jaboticabal, SP, Brazil.
José Augusto Senhorini
Affiliation:
Fish Biotechnology Laboratory, National Centre for Research and Continental Fish Conservation, Chico Mendes Institute of Biodiversity Conservation (CEPTA/ICMBio), Rodovia Pref. Euberto Nemesio Pereira de Godoy, Km 6,5, CEP 13630-970. Pirassununga-SP, Brazil.
Maria do Carmo Faria Paes
Affiliation:
Laboratory Animal Histology and Embryology, Department of Animal Morphology and Physiology, UNESP- São Paulo State University, Campus Jaboticabal-SP. Via de Acesso Prof. Paulo Donato Castellane s/n, CEP 14884-900 Jaboticabal, SP, Brazil.
Fernanda Nogueira Valentin
Affiliation:
UNESP's Aquaculture Centre (CAUNESP), UNESP – São Paulo State University, Campus Jaboticabal-SP. Via de Acesso Prof. Paulo Donato Castellane s/n, CEP 14884-900, Jaboticabal, SP, Brazil.
Takafumi Fujimoto
Affiliation:
Faculty of Fisheries Sciences, Hokkaido University, 3-1-1 Minato-cho, 041-8611 Hakodate, Japan.
Nivaldo Ferreira do Nascimento
Affiliation:
UNESP's Aquaculture Centre (CAUNESP), UNESP – São Paulo State University, Campus Jaboticabal-SP. Via de Acesso Prof. Paulo Donato Castellane s/n, CEP 14884-900, Jaboticabal, SP, Brazil.
George Shigueki Yasui
Affiliation:
Fish Biotechnology Laboratory, National Centre for Research and Continental Fish Conservation, Chico Mendes Institute of Biodiversity Conservation (CEPTA/ICMBio), Rodovia Pref. Euberto Nemesio Pereira de Godoy, Km 6,5, CEP 13630-970. Pirassununga-SP, Brazil.
Laura Satiko Okada Nakaghi
Affiliation:
UNESP's Aquaculture Centre (CAUNESP), UNESP – São Paulo State University, Campus Jaboticabal-SP. Via de Acesso Prof. Paulo Donato Castellane s/n, CEP 14884-900, Jaboticabal, SP, Brazil. Laboratory Animal Histology and Embryology, Department of Animal Morphology and Physiology, UNESP- São Paulo State University, Campus Jaboticabal-SP. Via de Acesso Prof. Paulo Donato Castellane s/n, CEP 14884-900 Jaboticabal, SP, Brazil.
*
All correspondence to: Regiane Cristina da Silva. UNESP's Aquaculture Centre (CAUNESP), UNESP – São Paulo State University, Campus Jaboticabal-SP. Via de Acesso Prof. Paulo Donato Castellane s/n, CEP 14884-900, Jaboticabal, SPBrazil. E-mail: [email protected]
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Summary

Primordial germ cell (PGC) transplant is a promising tool in aquaculture; however, successful use of this technique requires in depth knowledge of the early stages of embryo and larval development. The aim of this study was to analyse the effect of different temperatures (22, 26, and 30°C) on the early development of B. amazonicus. The newly fertilized eggs were distributed into tanks with controlled temperature and oxygenation. Samples were collected at pre-established times and analysed under light and fluorescence microscopy. Temperature influenced the speed and duration of each stage of early development, including hatching time. The highest pronuclei fusion rate was observed 8 min post-fertilization (mpf) at 22 and 26°C, and 6 mpf at 30°C. The duration of the 512–1000 blastomeres phase during in the blastocyst stage was 1 h 30 min at 22°C, and 25 min at 26 and 30°C. Hatching occurred at 24 h 30 mpf at 22°C, 16 h post-fertilization (hpf) at 26°C, and 11 h 30 mpf at 30°C. The rate of morphologically normal larvae was 88.34% at 22°C, 90.49% at 26°C, and 73% at 30°C. Malformations of the head, yolk sac, heart, and tail were observed in all temperatures. Nevertheless, B. amazonicus embryos were able to develop satisfactory in all three temperatures tested. These results enable embryo manipulation at different temperatures to optimize the micromanipulation time of embryos and larvae for biotechnological studies.

Keywords

BlastulaCell transplantationChimeraEmbryoGerm cell
Type
Research Article
Information
Zygote , Volume 25 , Issue 5 , October 2017 , pp. 637 - 651
Copyright
Copyright © Cambridge University Press 2017 

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