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Effect of sperm preparation on development of bovine blastocyst in vitro

Published online by Cambridge University Press:  01 November 2016

Maria Celina Abraham ,
Anders Johannisson  and
Jane M. Morrell
Maria Celina Abraham*
Affiliation:
Division of Reproduction, Department of Clinical Sciences, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Ulls väg 26, Uppsala, Sweden.
Anders Johannisson
Affiliation:
Division of Reproduction, Department of Clinical Sciences, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Ulls väg 26, Uppsala, Sweden.
Jane M. Morrell
Affiliation:
Division of Reproduction, Department of Clinical Sciences, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Ulls väg 26, Uppsala, Sweden.
*
All correspondence to Maria Celina Abraham. Division of Reproduction, Department of Clinical Sciences, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Ulls väg 26, Uppsala, Sweden. Tel: +46 01867 11 62. E-mail: [email protected]
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Summary

Sperm preparation is an important step in the in vitro production of embryos. Centrifugation through colloids has been used to select normal sperm for assisted reproduction in several species. Animal models can sometimes be used as a preliminary step to investigate sperm preparation methods that are potentially of use for human fertility treatments. In this study bovine semen was prepared using three variants of the single-layer centrifugation sperm selection technique (Small, Mini, Mini-EP) with Bovicoll (Androcoll-B). Computer-assisted sperm motility analysis, the hypo-osmotic swelling test, and the sperm chromatin structure assay were performed on unselected (control) and SLC-selected sperm samples. Mini and Mini-EP gave the highest yield of motile spermatozoa, progressive motility and membrane integrity. In vitro fertilization trials were performed to investigate the fertilizing ability of the frozen–thawed bovine spermatozoa selected with Bovicoll. Mini-SLC (single-layer centrifugation) and swim-up (Control) were performed and cleavage rate and blastocyst rate did not differ significantly between groups. As there was a trend to an increased number of cells in blastocysts in the SLC group, the Mini-SLC method is at least as good as swim-up for selecting frozen–thawed bull spermatozoa for in vitro fertilization (IVF). This method could potentially be used to prepare human sperm for assisted reproduction.

Keywords

BovineComputer-assisted semen analysis (CASA)In vitro fertilization (IVF)In vitro production (IVP) of embryosSingle-layer centrifugationSperm chromatin structure assay (SCSA)
Type
Research Article
Information
Zygote , Volume 24 , Issue 6 , December 2016 , pp. 825 - 830
Copyright
Copyright © Cambridge University Press 2016 

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