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Effect of oestrogen on mouse follicle growth and meiotic resumption

Published online by Cambridge University Press:  27 October 2021

Heng Chi  and
Zuowu Cao Open the ORCID record for Zuowu Cao [Opens in a new window]
Heng Chi
Affiliation:
Department of Developmental and Regenerative Biology, Jinan University College of Life Science and Technology, Guangzhou, China
Zuowu Cao*
Affiliation:
Department of Developmental and Regenerative Biology, Jinan University College of Life Science and Technology, Guangzhou, China
*
Author for correspondence: Zuowu Cao. Department of Developmental and Regenerative Biology, Jinan University College of Life Science and Technology, Guangzhou, 510632, China. E-mail: [email protected]
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Summary

Many studies have shown that oestrogen affects late follicular development, but whether oestrogen is involved in other aspects of folliculogenesis remains unclear. In this study, two antagonists of oestrogen, tamoxifen and G15, were used to determine the effects of oestrogen on folliculogenesis. Mouse preantral follicles and cumulus–oocyte complexes (COCs) were cultured in vitro. The results showed that follicle growth stimulated using pregnant mare serum gonadotrophin (PMSG) was inhibited using tamoxifen, whether in vivo or in vitro. The average diameters, the maximum diameters of follicles and the numbers of follicles with a diameter of more than 300 μm decreased significantly following a 4-day culture with tamoxifen. G15, the antagonist of oestrogen via the membrane receptor, did not change follicular growth stimulated by PMSG in vitro. Results of in vitro maturation of COCs showed that germinal vesicle breakdown (GVBD) occurred spontaneously (95.1%) after 2 h in culture, and the GVBD ratio changed little with the addition of either oestrogen or 10 μM G15. However, first polar body (PBI) extrusion was driven by oestrogen markedly and supplementation with 10 μM G15 inhibited PBI extrusion (82.4% vs 55.0%) significantly. These results demonstrated that oestrogen promotes follicle growth through the nuclear receptor during follicle growth and then triggers the transition of metaphase to anaphase through the membrane receptor during meiotic resumption. So oestrogen plays a progressive role in the two phases of follicle growth and oocyte meiotic resumption.

Keywords

FolliculogenesisMeiotic resumptionOestrogenOocytePolar body
Type
Research Article
Information
Zygote , Volume 30 , Issue 3 , June 2022 , pp. 330 - 337
Copyright
© The Author(s), 2021. Published by Cambridge University Press

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