Hostname: page-component-848d4c4894-8kt4b Total loading time: 0 Render date: 2024-07-07T22:49:27.282Z Has data issue: false hasContentIssue false
  • English
  • Français

The effect of FF-MAS on porcine cumulus–oocyte complex maturation, fertilization and pronucleus formation in vitro

Published online by Cambridge University Press:  01 August 2006

Inger Faerge ,
Frantisek Strejcek ,
Jozef Laurincik ,
Detlef Rath ,
Heiner Niemann ,
Karl Schellander ,
Christine Rosenkranz ,
Poul Maddox Hyttel  and
Christian Grøndahl
Inger Faerge*
Affiliation:
Fertility Team, Novo Nordisk A/S, Copenhagen, Denmark.
Frantisek Strejcek
Affiliation:
Constantine the Philosopher University, Nitra, Slovak Republic.
Jozef Laurincik
Affiliation:
Constantine the Philosopher University, Nitra, Slovak Republic. Research Institute of Animal Production, Nitra, Slovak Republic.
Detlef Rath
Affiliation:
Research Institute of Animal Production, Nitra, Slovak Republic.
Heiner Niemann
Affiliation:
Research Institute of Animal Production, Nitra, Slovak Republic.
Karl Schellander
Affiliation:
Faculty of Agriculture, Institute of Animal Breeding Science, University of Bonn, Bonn, Germany.
Christine Rosenkranz
Affiliation:
University of Veterinary Medicine, Vienna, Austria.
Poul Maddox Hyttel
Affiliation:
Veterinary and Agricultural University, Copenhagen, Denmark.
Christian Grøndahl
Affiliation:
Fertility Team, Novo Nordisk A/S, Copenhagen, Denmark.
*
All correspondence to: Inger Faerge, DVM, PhD, Institute of Basic Veterinary Sciences, Groennegaardsvej 7, 1870 Frb C, Copenhagen, Denmark. Tel: +45 35283882. Fax: +45 35282548. e-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Summary

Follicular fluid meiosis-activating sterol (FF-MAS) has been isolated from the follicular fluid (FF) of several species including man. FF-MAS increases the quality of in vitro oocyte maturation, and thus the developmental potential of oocytes exposed to FF-MAS during in vitro maturation is improved. The aim of the present study was to investigate the effects of FF-MAS on porcine oocyte maturation and pronucleus formation in vitro. Porcine cumulus–oocyte complexes (COCs) were isolated from abattoir ovaries and in vitro matured for 48 h in NCSU 37 medium supplemented with 1 mg/l cysteine, 10 ng/ml epidermal growth factor and 50 μM 2-mercaptoethanol with or without 10% porcine follicular fluid (pFF). For the first 22 h, 1 mM db-cAMP and 10 I.E PMSG/hCG was added. The medium was supplemented with 1 μM, 3 μM, 10 μM, 30 μM or 100 μM FF-MAS dissolved in ethanol. After maturation the COCs were denuded mechanically using a fine glass pipette under constant pH and in vitro fertilized with fresh semen (5 × 105 spermatozoa/ml). The presumptive zygotes were evaluated 18 h after fertilization. The addition of pFF increased the monospermic as well as the polyspermic penetration of oocytes. In the absence of pFF, the addition of FF-MAS decreased the polyspermic penetration rate, whereas FF-MAS in combination with pFF decreased monospermic and increased polyspermic penetration. The degeneration rate of ova decreased in the presence of FF-MAS irrespective of the presence or absence of pFF. In the absence of pFF, FF-MAS at 3–10 μM increased the number of zygotes with advanced maternal pronuclear stages. In supraphysiological doses, i.e. 30–100 μM, FF-MAS dose-dependently and reversibly inhibited nuclear maturation in the absence of pFF.

Keywords

FF-MASPigPronucleus formationSterols
Type
Research Article
Information
Zygote , Volume 14 , Issue 3 , August 2006 , pp. 189 - 199
Copyright
Copyright © Cambridge University Press 2006

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Ainsworth, L., Tsang, B.K., Downey, B., Marcus, G.J. & Armstrong, D.T. (1980). Interrelationships between follicular fluid steroid levels, gonadotrophic stimuli, and oocyte maturation during preovulatory development of porcine follicles. Biol. Reprod. 23, 621–7.CrossRefGoogle Scholar
Bivens, M., Lindenthal, B., O'Brien, M.J., Wigglesworth, K., Blume, T., Grondahl, C. & Eppig, J.J. (2004). A synthetic analogue of meiosis-activating sterol (FF-MAS) is a potent agonist promoting meiotic maturation and preimplantation development of mouse oocytes maturing in vitro. Hum Reprod. 19, 2340–4.CrossRefGoogle Scholar
Byskov, A.G., Andersen, C.Y., Nordholm, L., Thøgersen, H., Guoliang, X., Wassmann, O., Andersen, J.V., Guddal, E. & Roed, T. (1995). Chemical structure of sterols that activate oocyte meiosis. Nature 374, 559–62.CrossRefGoogle Scholar
Byskov, A.G., Andersen, C.Y., Hossaini, A. & Guoliang, X. (1997). Cumulus cells of oocyte–cumulus complexes secrete a meiosis-activating substance when stimulated with FSH. Mol. Reprod. Dev. 46, 296–305.3.0.CO;2-K>CrossRefGoogle ScholarPubMed
Calvin, H., Grosshans, K. & Blake, E. (1986). Estimation and manipulation of glutathione levels in prepubertal mouse ovaries and ova: relevance to sperm nucleus transformation in the fertilized egg. Gamete Res. 14, 265–75.CrossRefGoogle Scholar
Cavilla, J.L., Kennedy, C.R., Baltsen, M., Klentzeris, L.D., Byskov, A.G. & Hartshorne, G.M. (2001). The effects of meiosis activating sterol on in vitro maturation and fertilization of human oocytes from stimulated and unstimulated ovaries. Hum. Reprod. 16, 547–55.CrossRefGoogle ScholarPubMed
Coy, P., Gadea, J., Romar, R., Matas, C. & Garcia, E. (2002). Effect of in vitro fertilization medium on the acrosome reaction, cortical reaction, zona pellucida hardening and in vitro development in pigs. Reproduction 124, 279–88.CrossRefGoogle ScholarPubMed
Cukurcam, S., Hegele-Hartung, C. & Eichenlaub-Ritter, U. (2003). Meiosis-activating sterol protects oocytes from precocious chromosome segregation. Hum. Reprod. 18, 1908–17.CrossRefGoogle ScholarPubMed
Donnay, I., Faerge, I., Grondahl, C., Verhaeghe, B., Sayoud, H., Ponderato, N., Galli, C. & Lazzari, G. (2004). Effect of prematuration, meiosis activating sterol and enriched maturation medium on the nuclear maturation and competence to development of calf oocytes. Theriogenology 15, 1093–107.CrossRefGoogle Scholar
Grøndahl, C., Ottesen, J.L., Lessl, M., Faarup, P., Murray, A., Grønvald, F.C., Hegele-Hartung, C. & Ahnfelt-Rønne, I. (1998). Meiosis-activating sterol promotes resumption of meiosis in mouse oocytes cultured in vitro in contrast to related oxysterols. Biol. Reprod. 58, 1297–302.CrossRefGoogle ScholarPubMed
Hegele-Hartung, C., Lessl, M., Ottesen, J. & Grøndahl, C. (1998). Oocyte maturation can be induced by a synthetic meiosis activating sterol (MAS) leading to improvement of IVF rate in mice. Hum. Reprod. Suppl. 13, 98.Google Scholar
Hegele-Hartung, C., Kuhnke, J., Lessl, M., Grøndahl, C., Ottesen, J., Beier, H.M., Eisner, S. & Eichenlaub-Ritter, U. (1999). Nuclear and cytoplasmic maturation of mouse oocytes after treatment with synthetic meiosis-activating sterol in vitro. Biol. Reprod. 61, 1362–72.CrossRefGoogle ScholarPubMed
Hsu, C., Holmes, S. & Hammond, J. (1987). Ovarian epidermal growth factor-like activity: concentrations in the porcine follicular fluid during follicular enlargement. Gamete Res. 147, 242–7.Google Scholar
Hunter, R. (1967). Polyspermic fertilization in pigs during luteal phase of the estrous cycle. J. Exp. Zool. 165, 451–66.CrossRefGoogle ScholarPubMed
Ju, J.C., Tsay, C. & Ruan, C.W. (2003). Alterations and reversibility in the chromatin, cytoskeleton and development of pig oocytes treated with roscovitine. Mol. Reprod. Dev. 64, 482–91.CrossRefGoogle ScholarPubMed
Laurincik, J., Rath, D. & Niemann, H. (1994). Differences in pronucleus formation and first cleavage following in vitro fertilization between pig oocytes matured in vivo and in vitro. J. Reprod. Fertil. 102, 277–84.CrossRefGoogle ScholarPubMed
Laurincik, J., Hyttel, P. & Kopecny, V. (1995). DNA synthesis and pronucleus development in pig zygotes obtained in vivo: an autoradiographic and ultrastructural study. Mol. Reprod. Dev. 40, 325–32.Google ScholarPubMed
Le Beux, G., Richard, F.J. & Sirard, M.A. (2003). Effect of cycloheximide, 6-DMAP, roscovitine and butyrolactone I on resumption of meiosis in porcine oocytes. Theriogenology 1, 1049–58.CrossRefGoogle Scholar
Mattioli, M., Galeati, G. & Seren, E. (1988). Effect of follicle somatic cells during pig oocyte maturation on egg penetration and male pronucleus formation. Gamete Res. 20, 177–83.CrossRefGoogle ScholarPubMed
Mattioli, M., Bacci, M.L., Galeati, G. & Seren, E. (1989). Developmental competence of pig oocytes matured and fertilized in vitro. Theriogenology 31, 1201–7.CrossRefGoogle ScholarPubMed
Naito, K., Fukuda, Y. & Toyodam, Y. (1988). Effects of porcine follicular fluid on male pronucleus formation in porcine oocytes matured in vitro. Gamete Res. 21, 289–95.CrossRefGoogle ScholarPubMed
Petters, R.M. & Wells, K.D. (1993). Culture of pig embryos. J. Reprod. Fertil. Suppl. 48, 61–73.Google ScholarPubMed
Pincus, G. & Enzmann, E.V. (1935). The comparative behavior of mammalian eggs in vivo and in vitro. I. The activation of ovarian eggs. J. Exp. Med. 62, 655–75.CrossRefGoogle ScholarPubMed
Rath, D., Long, C.R., Dobrinsky, J., Welsh, G., Schreier, L.L. & Johnson, L. (1999). In vitro production of sexed embryos for gender preselection: high-speed sorting of X-chromosome-bearing sperm to produce pigs after embryo transfer.J. Anim. Sci. 77, 3346–52.CrossRefGoogle ScholarPubMed
Schroepfer, G.J. (1982). Sterol biosynthesis. Annu. Rev. Biochem. 51, 555–85.CrossRefGoogle ScholarPubMed
Stromstedt, M., Rozman, D. & Waterman, M.R. (1996). The ubiquitously expressed human CYP51 encodes lanosterol 14α-demethylase, a cytochrome P450 whose expression is regulated by oxysterols. Arch. Biochem. Biophys. 329, 73–81.CrossRefGoogle Scholar
Yoshida, M., Ishizaki, Y. & Kawagishi, H. (1990). Blastocyst formation by pig embryos resulting from in vitro fertilization of oocytes matured in vitro. J. Reprod. Fertil. 88, 1–8.CrossRefGoogle Scholar
Yoshida, M., Ishizaki, Y., Kawagishi, H., Bamba, K. & Kojima, Y. (1992). Effects of pig follicular fluid on maturation of pig oocytes in vitro and on their subsequent fertilizing and developmental capacity in vitro. J. Reprod. Fertil. 95, 481–8.CrossRefGoogle ScholarPubMed