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Effect of androstenedione on the growth and meiotic competence of bovine oocytes from early antral follicles

Published online by Cambridge University Press:  09 November 2011

Hiroaki Taketsuru ,
Yuji Hirao ,
Naoki Takenouchi ,
Kosuke Iga  and
Takashi Miyano
Hiroaki Taketsuru*
Affiliation:
Laboratory of Reproductive Biology, Graduate School of Agricultural Science, Kobe University, Nada-ku, Kobe 657–8501, Japan.
Yuji Hirao
Affiliation:
National Agricultural Research Center for Tohoku Region, Morioka, Iwate 020–0198, Japan.
Naoki Takenouchi
Affiliation:
National Agricultural Research Center for Tohoku Region, Morioka, Iwate 020–0198, Japan.
Kosuke Iga
Affiliation:
National Agricultural Research Center for Tohoku Region, Morioka, Iwate 020–0198, Japan.
Takashi Miyano
Affiliation:
Graduate School of Agricultural Science, Kobe University, Kobe 657–8501, Japan.
*
All correspondence to: Hiroaki Taketsuru. Laboratory of Reproductive Biology, Graduate School of Agricultural Science, Kobe University, Nada-ku, Kobe 657–8501, Japan. Tel: +81 78 803 5806. Fax: +81 78 803 5807. e-mail: [email protected]
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Summary

Medium that contains 17β-estradiol has been reported to support in vitro growth of bovine oocytes, isolated from early antral follicles, until the final stage. The aim of this study was to determine the effects of androstenedione in medium on such growing bovine oocytes. Oocyte–granulosa cell complexes were collected from early antral follicles and cultured for 14 days in medium supplemented with 17β-estradiol (0, 10 and 100 ng/ml) or androstenedione (0, 10 and 100 ng/ml). The mean diameter of oocytes measured after seeding on the culture substrate was 96.9 μm (n = 191). Either steroid was necessary for maintainance of the organization of oocyte–granulosa cell complexes over the 14-day culture period. In the 17β-estradiol- or the androstenedione-supplemented medium about 80% or 65%, respectively, of viable oocytes were recovered. In both groups the increase in oocyte size was significant after 14 days. The in vitro grown oocytes were cultured for a further 22–24 h for oocyte maturation; 13% and 30% of oocytes grown in the 10 and 100 ng/ml 17β-estradiol-supplemented medium reached metaphase II, respectively; more than 64% of oocytes grown in the androstenedione-supplemented medium matured to metaphase II. These results show that androstenedione, as 17β-estradiol, can maintain the viability of bovine oocyte–granulosa cell complexes and support the growth of oocytes, and that androstenedione promotes the acquisition of oocyte meiotic competence efficiently at a low dose.

Keywords

AndrostenedioneBovineEarly antral follicle17β-EstradiolOocyte growth
Type
Research Article
Information
Zygote , Volume 20 , Issue 4 , November 2012 , pp. 407 - 415
Copyright
Copyright © Cambridge University Press 2011

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References

Anderson, E. & Albertini, D.F. (1976). Gap junctions between the oocyte and companion follicle cells in the mammalian ovary. J. Cell Biol. 71, 680–6.CrossRefGoogle ScholarPubMed
Cain, L., Chatterjee, S. & Collins, T.J. (1995). In vitro folliculogenesis of rat preantral follicles. Endocrinology 136, 3369–77.CrossRefGoogle ScholarPubMed
Daniel, S.A. & Armstrong, D.T. (1980). Enhancement of follicle-stimulating hormone-induced aromatase activity by androgens in cultured rat granulosa cells. Endocrinology 107, 1027–33.CrossRefGoogle ScholarPubMed
Demeestere, I., Delbaere, A., Gervy, C., Van Den Bergh, M., Devreker, F. & Englert, Y. (2002). Effect of preantral follicle isolation technique on in-vitro follicular growth, oocyte maturation and embryo development in mice. Hum. Reprod. 17, 2152–9.CrossRefGoogle ScholarPubMed
Eppig, J.J., O'Brien, M. & Wigglesworth, K. (1996). Mammalian oocyte growth and development in vitro. Mol. Reprod. Dev. 44, 260–73.3.0.CO;2-6>CrossRefGoogle ScholarPubMed
Eppig, J.J. & Schroeder, A.C. (1989). Capacity of mouse oocytes from preantral follicles to undergo embryogenesis and development to live young after growth, maturation, and fertilization in vitro. Biol. Reprod. 41, 268–76.CrossRefGoogle ScholarPubMed
Fair, T., Hyttel, P. & Greve, T. (1995). Bovine oocyte diameter in relation to maturational competence and transcriptional activity. Mol. Reprod. Dev. 42, 437–42.CrossRefGoogle ScholarPubMed
Fortune, J.E. & Armstrong, D.T. (1978). Hormonal control of 17 beta-estradiol biosynthesis in proestrous rat follicles: estradiol production by isolated theca versus granulosa. Endocrinology 102, 227–35.CrossRefGoogle ScholarPubMed
Gilchrist, R.B., Ritter, L.J. & Armstrong, D.T. (2001). Mouse oocyte mitogenic activity is developmentally coordinated throughout folliculogenesis and meiotic maturation. Dev. Biol. 240, 289–98.CrossRefGoogle ScholarPubMed
Harada, M., Miyano, T., Matsumura, K., Osaki, S., Miyake, M. & Kato, S. (1997). Bovine oocytes from early antral follicles grow to meiotic competence in vitro: effect of FSH and hypoxanthine. Theriogenology 48, 743–55.CrossRefGoogle ScholarPubMed
Hashimoto, S., Ohsumi, K., Tsuji, Y., Harauma, N., Miyata, Y., Fukuda, A., Hosoi, Y., Iritani, A. & Morimoto, Y. (2007). Growing porcine oocyte–granulosa cell complexes acquired meiotic competence during in vitro culture. J. Reprod. Dev. 53, 379–84.CrossRefGoogle ScholarPubMed
Hirao, Y., Itoh, T., Shimizu, M., Iga, K., Aoyagi, K., Kobayashi, M., Kacchi, M., Hoshi, H. & Takenouchi, N. (2004). In vitro growth and development of bovine oocyte–granulosa cell complexes on the flat substratum: effects of high polyvinylpyrrolidone concentration in culture medium. Biol. Reprod. 70, 83–91.CrossRefGoogle ScholarPubMed
Iwata, H., Ohota, M., Hashimoto, S. & Nagai, Y. (2003). Free oxygen radicals are generated at the time of aspiration of oocytes from ovaries that have been stored for a long time. Zygote 11, 1–5.CrossRefGoogle ScholarPubMed
Liu, Y.X. & Hsueh, A.J. (1986). Synergism between granulosa and theca-interstitial cells in estrogen biosynthesis by gonadotropin-treated rat ovaries: studies on the two-cell, two-gonadotropin hypothesis using steroid antisera. Biol. Reprod. 35, 27–36.CrossRefGoogle ScholarPubMed
Miyano, T. (2005). In vitro growth of mammalian oocytes. J. Reprod. Dev. 51, 169–76.CrossRefGoogle ScholarPubMed
Sato, E., Matsuo, M. & Miyamoto, H. (1990). Meiotic maturation of bovine oocytes in vitro: improvement of meiotic competence by dibutyryl cyclic adenosine 3′,5′-monophosphate. J. Anim. Sci. 68, 1182–7.CrossRefGoogle ScholarPubMed
Schreiber, J.R. & Ross, G.T. (1976). Further characterization of a rat ovarian testosterone receptor with evidence for nuclear translocation. Endocrinology 99, 590–6.CrossRefGoogle ScholarPubMed
Sen, A. & Hammes, S.R. (2010). Granulosa cell-specific androgen receptors are critical regulators of ovarian development and function. Mol. Endocrinol. 24, 1393–403.CrossRefGoogle ScholarPubMed
Shen, X., Miyano, T. & Kato, S. (1998). Promotion of follicular antrum formation by pig oocytes in vitro. Zygote 6, 47–54.CrossRefGoogle ScholarPubMed
Walters, K.A., Allan, C.M. & Handelsman, D.J. (2008). Androgen actions and the ovary. Biol. Reprod. 78, 380–9.CrossRefGoogle ScholarPubMed
Yamamoto, K., Otoi, T., Koyama, N., Horikita, N., Tachikawa, S. & Miyano, T. (1999). Development to live young from bovine small oocytes after growth, maturation and fertilization in vitro. Theriogenology 52, 81–9.CrossRefGoogle ScholarPubMed