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The effect of 6-dimethylaminopurine (6-DMAP) on DNA synthesis in activated mammalian oocytes

Published online by Cambridge University Press:  26 September 2008

S. Ledda ,
P. Loi ,
L. Bogliolo ,
R.M. Moor  and
J. Fulka Jr
S. Ledda
Affiliation:
University of Sassari, Italy, Institute of Ziitechnics, Olmedo, Italy, The Babraham Institute, Cambridge, UK, and Institute of Animal Production, Prague, Czech Republic.
P. Loi
Affiliation:
University of Sassari, Italy, Institute of Ziitechnics, Olmedo, Italy, The Babraham Institute, Cambridge, UK, and Institute of Animal Production, Prague, Czech Republic.
L. Bogliolo
Affiliation:
University of Sassari, Italy, Institute of Ziitechnics, Olmedo, Italy, The Babraham Institute, Cambridge, UK, and Institute of Animal Production, Prague, Czech Republic.
R.M. Moor
Affiliation:
University of Sassari, Italy, Institute of Ziitechnics, Olmedo, Italy, The Babraham Institute, Cambridge, UK, and Institute of Animal Production, Prague, Czech Republic.
J. Fulka Jr*
Affiliation:
University of Sassari, Italy, Institute of Ziitechnics, Olmedo, Italy, The Babraham Institute, Cambridge, UK, and Institute of Animal Production, Prague, Czech Republic.
*
J. Fulka, Jr, Institute of Animal Production, CS-104 00 Prague 10 – Uhříněves, Czech Republic. Telephone: 42 267710659. Fax: 42 267710779.
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Summary

Mouse, sheep and bovine metaphase II oocytes were briefly preincubated in medium with 6-dimethyl-aminopurine (6-DMAP) and thereafter activated with ethanol before further culture in 6-DMAP supplemented medium. The presence of 6-DMAP enhances the efficiency of activation and the speed of pronuclear formation but has no effect on DNA synthesis. These results are discussed in the context of recently published data showing that the preincubation of condensed chromosomes with 6-DMAP blocks DNA synthesis when nuclei become reformed.

Keywords

ActivationDNA synthesisOocytes
Type
Article
Information
Zygote , Volume 4 , Issue 1 , February 1996 , pp. 7 - 9
Copyright
Copyright © Cambridge University Press 1996

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