Hostname: page-component-586b7cd67f-dlnhk Total loading time: 0 Render date: 2024-11-22T15:19:58.543Z Has data issue: false hasContentIssue false
  • English
  • Français

DNA fragmentation index, pAKT and pERK1/2 in cumulus cells are related to oocyte competence in patients undergoing in vitro fertilization programme

Published online by Cambridge University Press:  14 August 2019

Giovanni Ruvolo ,
Maria Carmela Roccheri ,
Claudio Luparello ,
Domenica Matranga ,
Alberto Ferrigno  and
Liana Bosco Open the ORCID record for Liana Bosco [Opens in a new window]
Giovanni Ruvolo
Affiliation:
Centro di Biologia della Riproduzione, Via Villareale Palermo, Sicily
Maria Carmela Roccheri
Affiliation:
Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche. Università degli Studi di Palermo, Viale delle Scienze Ed.16, Palermo, Sicily
Claudio Luparello
Affiliation:
Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche. Università degli Studi di Palermo, Viale delle Scienze Ed.16, Palermo, Sicily
Domenica Matranga
Affiliation:
Department of Health Promotion Sciences Maternal and Infant Care, Internal Medicine and Medical Specialities, University of Palermo, Palermo, Italy
Alberto Ferrigno
Affiliation:
Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche. Università degli Studi di Palermo, Viale delle Scienze Ed.16, Palermo, Sicily
Liana Bosco*
Affiliation:
Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche. Università degli Studi di Palermo, Viale delle Scienze Ed.16, Palermo, Sicily
*
Address for correspondence: Liana Bosco. Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche. Università degli Studi di Palermo, Viale delle Scienze Ed.16, Palermo, Sicily. Tel: +39 09123897411. Fax: +39 0916577210. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Summary

Activated pERK1/2 and pAKT are key players in supporting cell survival and proliferation pathways. Translocation of pERK1/2 into the nucleus, where it interacts with transcription factors and DNA itself, is instrumental in exerting an anti-apoptotic effect. In this study, pAKT levels, pERK1/2 nuclear localization and DNA fragmentation index (DFI) in cumulus cells of single cumulus–oocyte complexes of patients undergoing in vitro fertilization programmes were evaluated and correlated with the clinical outcome of the related embryos. For a positive clinical outcome of blastocyst development, pERK1/2 nuclear localization and DFI value had a significant inverse relationship, whereas the former and the intracellular accumulation of pAKT had a significant direct relationship. This relationship was not observed for the negative clinical outcome of the arrested embryos. Moreover, intracellular accumulation of pAKT and DFI value had a significant inverse relationship in all samples examined. The obtained data suggest that the intranuclear relocation of pERK1/2, along with an enhanced intracellular accumulation of pAKT, may exert a survival effect and increase cell viability, therefore providing a novel marker tool to choose the best oocyte to be fertilized and submitted to an intracytoplasmic sperm injection cycle.

Keywords

ApoptosisCell survivalDFIMolecular markersOocyte quality
Type
Short Communication
Information
Zygote , Volume 27 , Issue 5 , October 2019 , pp. 350 - 354
Copyright
© Cambridge University Press 2019 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Agnello, M, Bosco, L, Chiarelli, R, Martino, C and Roccheri, MC (2015) The role of autophagy and apoptosis during embryo development. In: Ntuli, T (ed.) Cell Death – Autophagy, Apoptosis and Necrosis. InTech Ltd, London, pp. 83–112.Google Scholar
Bosco, L, Roccheri, MC, Martino, C, Chiarelli, R, Lispi, M and Ruvolo, G (2017a) Apoptosis rate in cumulus cells as possible molecular biomarker for oocyte competence. EuroMed Biomed J 12, 51–6.Google Scholar
Bosco, L, Chiarelli, R, Roccheri, MC, Matranga, D and Ruvolo, G (2017b) Relationship between apoptosis and survival molecules in human cumulus cells as markers of oocyte competence. Zygote 25, 583–91.CrossRefGoogle ScholarPubMed
Bosco, L, Ruvolo, G, Luparello, C, Ferrari, S, Valerio, D, Santi, D, Piomboni, P, Sarcina, E, Lispi, M and Roccheri, MC (2017c) Gene expression and apoptosis levels in cumulus cells of patients with polymorphisms of FSHR and LHB undergoing in vitro fertilization program. Cell Physiol Biochem 43, 2391–404.CrossRefGoogle ScholarPubMed
Casar, B, Pinto, A and Crespo, P (2009) ERK dimers and scaffold proteins: unexpected partners for a forgotten (cytoplasmic) task. Cell Cycle 8, 1007–13.CrossRefGoogle ScholarPubMed
Choi, SK, Galán, M, Kassan, M, Partyka, M, Trebak, M and Matrougui, K (2012) Poly(ADP-ribose) polymerase 1 inhibition improves coronary arteriole function in type 2 diabetes mellitus. Hypertension 59, 1060–8.CrossRefGoogle ScholarPubMed
Du, M, Fu, X and Zhou, Y (2013) Effects of trichostatin A on cumulus expansion during mouse oocyte maturation. Asian-Australas J Anim Sci 26, 1545–52.CrossRefGoogle ScholarPubMed
Duronio, V (2008) The life of a cell: apoptosis regulation by the PI3K/PKB pathway. Biochem J 415, 333–44.CrossRefGoogle ScholarPubMed
El-Hayek, S and Clarke, HJ (2016) Control of oocyte growth and development by intercellular communication within the follicular niche. Results Probl Cell Differ 58, 191–224.CrossRefGoogle ScholarPubMed
Librizzi, M, Chiarelli, R, Bosco, L, Sansook, S, Gascon, JM, Spencer, J, Caradonna, F and Luparello, C (2015) The histone deacetylase inhibitor JAHA down-regulates pERK and global DNA methylation in MDA-MB231 breast cancer cells. Materials 8, 7041–7.CrossRefGoogle ScholarPubMed
Mebratu, Y and Tesfaigzi, Y (2009) How ERK1/2 activation controls cell proliferation and cell death: Is subcellular localization the answer? Cell Cycle 8, 1168–75.CrossRefGoogle Scholar
Plotnikov, A, Flores, K, Maik-Rachline, G, Zehorai, E, Kapri-Pardes, E, Berti, DA, Hanoch, T, Besser, MJ and Seger, R (2015) The nuclear translocation of ERK1/2 as an anticancer target. Nat Commun 6, 6685.CrossRefGoogle ScholarPubMed
Ruvolo, G, Bosco, L, Pane, A, Morici, G, Cittadini, E and Roccheri, MC (2007) Lower apoptosis rate in human cumulus cells after administration of recombinant luteinizing hormone to women undergoing ovarian stimulation for in vitro fertilization procedures. Fertil Steril 87, 542–6.CrossRefGoogle ScholarPubMed
Ruvolo, G, Fattouh, RR, Bosco, L, Brucculeri, AM and Cittadini, E (2013) New molecular markers for the evaluation of gamete quality. J Assist Reprod Genet 30, 207–12.CrossRefGoogle ScholarPubMed
Shiota, M, Sugai, N, Tamura, M, Yamaguchi, R, Fukushima, N, Miyano, T and Miyazaki, H (2003) Correlation of mitogen-activated protein kinase activities with cell survival and apoptosis in porcine granulosa cells. Zoolog Sci 20, 193–201.CrossRefGoogle ScholarPubMed
Stanciu, M and De Franco, DB (2002) Prolonged nuclear retention of activated extracellular signal-regulated protein kinase promotes cell death generated by oxidative toxicity or proteasome inhibition in a neuronal cell line. J Biol Chem 277, 4010–7.CrossRefGoogle ScholarPubMed
Wainstein, E and Seger, R (2016) The dynamic subcellular localization of ERK: mechanisms of translocation and role in various organelles. Curr Opin Cell Biol 39, 15–20.CrossRefGoogle ScholarPubMed
Xu, B, Zhang, YW, Tong, XH and Liu, YS (2015) Characterization of microRNA profile in human cumulus granulosa cells: Identification of microRNAs that regulate Notch signaling and are associated with PCOS. Mol Cell Endocrinol 404, 26–36.CrossRefGoogle ScholarPubMed
Zuccotti, M, Merico, V, Cecconi, S, Redi, CA and Garagna, S (2011) What does it take to make a developmentally competent mammalian egg? Hum Reprod Update 17, 525–40.CrossRefGoogle ScholarPubMed