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Developmental capacity of Antarctic minke whale (Balaenoptera bonaerensis) vitrified oocytes following in vitro maturation, and parthenogenetic activation or intracytoplasmic sperm injection

Published online by Cambridge University Press:  01 May 2006

Takuma Fujihira ,
Mariko Kobayashi ,
Shinichi Hochi ,
Masumi Hirabayashi ,
Hajime Ishikawa ,
Seiji Ohsumi  and
Yutaka Fukui
Takuma Fujihira
Affiliation:
Laboratory of Animal Reproduction, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan.
Mariko Kobayashi
Affiliation:
Laboratory of Animal Reproduction, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan.
Shinichi Hochi
Affiliation:
Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano 386-8567, Japan.
Masumi Hirabayashi
Affiliation:
National Institute for Physiological Sciences, Okazaki, Aichi 444-8787, Japan.
Hajime Ishikawa
Affiliation:
The Institute of Cetacean Research, Tokyo 104-0055, Japan.
Seiji Ohsumi
Affiliation:
The Institute of Cetacean Research, Tokyo 104-0055, Japan.
Yutaka Fukui*
Affiliation:
Laboratory of Animal Reproduction, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan.
*
All correspondence to: Y. Fukui. Fax: +81 155 495593. e-mail: [email protected]
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Summary

The present study investigated the effects of the sexual maturity of oocyte donors on in vitro maturation (IVM) and the parthenogenetic developmental capacity of fresh minke whale oocytes. The effects of cytochalasin B (CB) pretreatment and two types of cryoprotectant solutions (ethylene glycol (EG) or ethylene glycol and dimethylsulfoxide (EG + DMSO)) on the in vitro maturation of vitrified immature whale oocytes were compared, and the developmental capacity of vitrified immature whale oocytes following IVM and intracytoplasmic sperm injection examined (ICSI). The maturation rate did not differ significantly with sexual maturity (adult, 60.9%; prepubertal, 53.1%), but the parthenogenetic activation rate of oocytes from adult donors (76.7%) was significantly higher (p < 0.05) than that of oocytes from prepubertal donors (46.4%). The maturation rates after vitrification and warming were not significantly different between the EG (22.2%) and EG + DMSO groups (30.2%), or between the CB-treated (30.4%) and non-CB-treated groups (27.3%). These results indicate that parthenogenetic activation of in vitro matured oocytes from adult minke whales was superior to that from prepubertal whales, but that the developmental capacity of the whale oocytes after parthenogenetic activation or ICSI was still low. The present study also showed that CB treatment before vitrification and two kinds of cryoprotectants did not improve the IVM rate following the vitrification of immature whale oocytes.

Keywords

ICSIIVMMinke whale oocyteParthenogenetic activationVitrification
Type
Research Article
Information
Zygote , Volume 14 , Issue 2 , May 2006 , pp. 89 - 95
Copyright
Copyright © Cambridge University Press 2006

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