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Development of prepubertal goat oocytes after their in vitro maturation and chemical activation

Published online by Cambridge University Press:  10 August 2020

Seungbum Hong ,
Binoy S. Vettical  and
Nisar Ahmad Wani Open the ORCID record for Nisar Ahmad Wani [Opens in a new window]
Seungbum Hong
Affiliation:
Reproductive Biotechnology Centre, Post Box 299003, Dubai, United Arab Emirates
Binoy S. Vettical
Affiliation:
Reproductive Biotechnology Centre, Post Box 299003, Dubai, United Arab Emirates
Nisar Ahmad Wani*
Affiliation:
Reproductive Biotechnology Centre, Post Box 299003, Dubai, United Arab Emirates
*
Author for correspondence: Nisar Ahmad Wani. Reproductive Biotechnology Centre, Post Box 299003, Dubai, United Arab Emirates. Tel: +971 4 327 2568. Fax: + 971 4 327 2563. E-mail: [email protected]
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Summary

Experiments were conducted to study in vitro maturation of prepubertal goat oocytes and their developmental potential after chemical activation. In Experiment 1, cumulus–oocytes complexes collected from the ovaries of prepubertal goats slaughtered at a local abattoir were matured in vitro in TCM-199-based medium supplemented with 10 µg/ml luteinizing hormone (LH) (treatment 1) or 10 µg/ml LH + 0.1 mM l-cysteine (treatment 2). In Experiment 2, mature oocytes were activated with either 5 µM ionomycin or 7% ethanol. After 18 h, some oocytes were randomly fixed and stained to evaluate their chromatin status, while others were cultured in embryo culture medium to study their further development. In Experiment 3, oocytes activated with 5 µM ionomycin were cultured for 7 days in one of the four different culture media [Charles Rosenkrans medium (CR-1), TCM-199, potassium simplex optimization medium (KSOM) and synthetic oviductal fluid (SOF)] to study their developmental potential. The maturation rate in control, treatment 1, and treatment 2 media did not differ from each other (P > 0.05). However, the lowest degeneration of oocytes was observed in treatment 3 (P < 0.05) when compared with the other two groups. The proportion of activated oocytes was higher, while non-activated oocytes were lower in ionomycin group when compared with the group activated with ethanol (P < 0.05). The proportions of oocytes cleaved were 65.7, 56.8, 61.0 and 54.4% in CR-1, TCM-199, KSOM and SOF medium, respectively, with no significant difference. However, further development of cleaved oocytes was better in KSOM followed by SOF.

Keywords

Chemical activationIn vitro cultureIn vitro maturationPrepubertal goat oocytes
Type
Research Article
Information
Zygote , Volume 28 , Issue 6 , December 2020 , pp. 447 - 452
Copyright
© The Author(s), 2020. Published by Cambridge University Press

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Footnotes

*

Part of the results presented in this manuscript, were reported at the Annual Conference of the International Embryo Technology Society, 20–23 January 2018, New Orleans, USA.

References

Abazari-Kia, AH, Mohammadi-Sangcheshmeh, A, Dehghani-Mohammadabadi, M, Jamshidi-Adegani, F, Veshkini, A, Zhandi, M, Cinar, MU and Salehi, M (2014). Intracellular glutathione content, developmental competence and expression of apoptosis-related genes associated with G6PDH-activity in goat oocyte. J Assist Reprod Genet 31, 313–21.CrossRefGoogle ScholarPubMed
Abramczuk, J, Solter, D and Koprowski, H (1977). The beneficial effect EDTA on development of mouse one-cell embryos in chemically defined medium. Dev Biol 61, 378–83.CrossRefGoogle ScholarPubMed
Biggers, JD and Summers, MC (2008). Choosing a culture medium: making informed choices. Fertil Steril 90, 473–83.CrossRefGoogle ScholarPubMed
Cuthbertson, KS, Whittingham, DG and Cobbold, PH (1981). Free Ca2+ increases in exponential phases during mouse oocyte activation. Nature 294, 754–7.CrossRefGoogle ScholarPubMed
De Matos, DG, Furnus, CC and Moses, DF (1997). Glutathione synthesis during in vitro maturation of bovine oocytes: role of cumulus cells. Biol Reprod 57, 1420–5.CrossRefGoogle ScholarPubMed
Eppig, JJ (1996). Coordination of nuclear and cytoplasmic oocyte maturation in eutherian mammals. Reprod Fert Dev 8, 485–9.CrossRefGoogle ScholarPubMed
Gasparrini, B, Boccia, L, Rosa, AD, Palo, RD, Campanile, G and Zicarelli, L (2004). Chemical activation of buffalo (Bubalus bubalis) oocytes by different methods: effects of aging on post-parthenogenetic development. Theriogenology 62, 1627–37.CrossRefGoogle ScholarPubMed
Izquierdo, D, Villamediana, P and Paramio, MT (1999). Effect of culture media on embryo development from prepubertal goat IVM–IVF oocytes. Theriogenology 52, 847–61.CrossRefGoogle ScholarPubMed
Jiménez-Macedo, AR, Izquierdo, D, Anguita, B and Paramio, MT (2005). Comparison between intracytoplasmic sperm injection and in vitro fertilisation employing oocytes derived from prepubertal goats. Theriogenology 64, 1249–62.CrossRefGoogle ScholarPubMed
Jones, KT, Carroll, J, and Whittingham, DG (1995). Ionomycin, thapsigargin, ryanodine, and sperm induced Ca2+ release increase during meiotic maturation of mouse oocytes. J Biol Chem 270, 6671–7.CrossRefGoogle ScholarPubMed
Junk, SM, Dharmarajan, A and Yovich, JL (2003). FSH priming improves oocytes maturation but priming with RSH or hCG has no effect on subsequent embryonic development in an in vitro maturation program. Theriogenology 59, 1741–1749.CrossRefGoogle Scholar
Khatun, M, Bhuiyan, MM, Ahmed, JU, Haque, A, Rahman, MB and Shamsuddin, M (2010). In vitro maturation and fertilization of prepubertal and pubertal black Bengal goat oocytes. J Vet Sci 12, 75–82.CrossRefGoogle Scholar
Kim, NH, Uhm, SJ, Ju, JY, Lee, HT and Chung, KS (1997). Blastocoele formation and cell allocation to the inner cell mass and trophectoderm in haploid and diploid pig parthenotes developing in vitro . Zygote 5, 365–70.CrossRefGoogle ScholarPubMed
Kline, D and Kline, JT (1992). Repetitive calcium transients and the role of calcium in exocytosis and cell cycle activation in the mouse egg. Dev Biol 149, 80–9.CrossRefGoogle ScholarPubMed
Lan, GC, Han, D, Wu, YG, Han, ZB, Ma, SF, Liu, XY, Chang, CL and Tan, JH (2005). Effects of duration, concentration, and timing of ionomycin and 6-dimethylaminopurine (6-DMAP) treatment on activation of goat oocytes. Mol Reprod Dev 71, 380–8.CrossRefGoogle ScholarPubMed
Lawitts, JA and Biggers, JD (1991). Optimization of mouse embryo culture media using simplex methods. J Reprod Fertil 91, 543–56.CrossRefGoogle ScholarPubMed
Liu, RH, Sun, QY, Li, YH, Jiao, LH and Wang, WH (2003). Maturation of porcine oocytes after cooling at the germinal vesicle stage. Zygote 11, 299–305.CrossRefGoogle ScholarPubMed
Lorca, T, Cruzalegui, FH, Fesquet, D, Cavadore, JC, Méry, J, Means, A and Dorée, M (1993). Calmodulin-dependent protein kinase II mediates inactivation of MPF and CSF upon fertilization of Xenopus eggs. Nature 366, 270–3.CrossRefGoogle ScholarPubMed
Matsukawa, T, Ikeda, S, Imai, H and Yamada, M (2002). Alleviation of the two-cell block of ICR mouse embryos by polyaminocarboxylate metal chelators. Reproduction 124, 65–71.CrossRefGoogle ScholarPubMed
Nagai, T (1987). Parthenogenetic activation of cattle follicular oocytes in vitro with ethanol. Gamete Res 16, 243–9.CrossRefGoogle ScholarPubMed
Ongeri, EM, Bormann, CL, Butler, RE, Melican, D, Gavin, WG, Echelard, Y, Krisher, RL and Behboodi, E (2001). Development of goat embryos after in vitro fertilization and parthenogenetic activation by different methods. Theriogenology 55, 1933–45.CrossRefGoogle ScholarPubMed
Ozil, JP (1990). The parthenogenetic development of rabbit oocytes after repetitive pulsatile electrical stimulation. Development 109, 117–27.Google ScholarPubMed
Phillips, DM and Dekel, N (1982). Effect of gonadotropins and prostaglandin on cumulus mucification in cultures of intact follicles. J Exp Zool 221, 275–82.CrossRefGoogle ScholarPubMed
Purse, VG, Wall, RJ, Rexroad, CE, Hammer, RE and Brinster, RL (1985). A rapid whole-mount staining procedure for nuclei of mammalian embryos. Theriogenology 24, 687–91.CrossRefGoogle Scholar
Rho, GJ, Kawarsky, S, Johnson, WH, Kochhar, K and Betteridge, KJ (1998). Sperm and oocyte treatments to improve the formation of male and female pronuclei and subsequent development following intracytoplasmic sperm injection into bovine oocytes. Biol Reprod 59, 918–24.CrossRefGoogle ScholarPubMed
Rodríguez-González, E, López-Bejar, M, Mertens, MJ and Paramio, MT, (2003). Effects on in vitro embryo development and intracellular glutathione content of the presence of thiol compounds during maturation of prepubertal goat oocytes. Mol Reprod Dev 65, 446–53.CrossRefGoogle ScholarPubMed
Romaguera, R, Casanovas, A, Morató, R, Izquierdo, D, Catalá, M, Jiménez-Macedo, AR, Mogas, T and Paramio, MT (2010). Effect of follicle diameter on oocyte apoptosis, embryo development and chromosomal ploidy in prepubertal goats. Theriogenology 74, 364–73.CrossRefGoogle ScholarPubMed
Rosenkrans, CF Jr and First, NL (1994). Effect of free amino acids and vitamins on cleavage and developmental rate of bovine zygotes in vitro . J. Anim. Sci. 72, 434–7.CrossRefGoogle ScholarPubMed
Shiina, Y Kaneda, M Matsuyama, K Tanaka, K Hiroi, M and Doi, K (1993). Role of extracellular Ca2+ on the intracellular Ca2+ changes in fertilized and activated mouse oocytes. J Reprod Fertil 97, 143–50.CrossRefGoogle ScholarPubMed
Shimada, M, Nishibori, M, Isobe, N, Kawano, N and Terada, T (2003). Luteinizing hormone receptor formation in cumulus cells surrounding porcine oocytes and its role during meiotic maturation of porcine oocytes. Biol Reprod 68, 1142–9.CrossRefGoogle ScholarPubMed
Susko-Parrish, JL, Leibfried-Rutledge, ML, Northey, DL, Schutzkus, V and First, NL (1994). Inhibition of protein kinases after an induced calcium transient causes transition of bovine oocytes to embryonic cycles without meiotic completion. Dev Biol 166, 729–739.CrossRefGoogle ScholarPubMed
Talukder, AK, Shamsuddin, M, Rahman, MB, Bari, FY and Parrish, JJ (2009). Normal and abnormal fertilization of zebu cattle oocytes in vitro . J Embryo Transf 24, 89–95.Google Scholar
Tervit, HR, Whittingham, DG and Rowson, LE (1972). Successful culture of in vitro sheep and cattle ova. J Reprod Fertil 30, 493–7.CrossRefGoogle ScholarPubMed
Wani, NA (2002). In vitro maturation and in vitro fertilization of sheep oocytes. Small Rum Res 44, 89–95.CrossRefGoogle Scholar
Wani, NA (2008). Chemical activation of in vitro matured dromedary camel (Camelus dromedarius) oocytes: optimization of protocols. Theriogenology 69, 591–602.CrossRefGoogle ScholarPubMed
Wani, NA (2009). In vitro embryo production in camel (Camelus dromedarius) from in vitro matured oocytes fertilized with epididymal spermatozoa stored at 4C. Anim Reprod Sci 111, 69–79.CrossRefGoogle Scholar
Wani, NA and Nowshari, MA (2005). Kinetics of nuclear maturation and effect of holding ovaries at room temperature on in vitro maturation of camel (Camelus dromedarius) oocytes. Theriogenology 64, 75–85.CrossRefGoogle ScholarPubMed
Wani, NA, Wernery, U, Hassan, FA, Wernery, R and Skidmore, JA (2010). Production of the first cloned camel by somatic cell nuclear transfer. Biol Reprod 82, 373–9.CrossRefGoogle ScholarPubMed
Younis, AI, Zuelke, KA, Harper, KM, Oliveira, MAL and Brackett, BG (1991). In vitro fertilization of goat oocytes. Biol Reprod 44, 1177–82.CrossRefGoogle ScholarPubMed
Zhou, P, Wu, YG, Li, Q, Lan, GC, Wang, G, Gao, D and Tan, JH (2008). The interactions between cysteamine, cystine and cumulus cells increase the intracellular glutathione level and developmental capacity of goat cumulus-denuded oocytes. Reproduction 135, 605–11.CrossRefGoogle ScholarPubMed
Zuelke, KA and Brackett, BG (1990). Luteinizing hormone enhanced in vitro maturation of bovine oocytes with and without protein supplementation. Biol Reprod 43, 784–7.CrossRefGoogle ScholarPubMed