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Development, molecular composition and freeze tolerance of bovine embryos cultured in TCM-199 supplemented with hyaluronan

Published online by Cambridge University Press:  01 February 2008

A.T. Palasz ,
P. Beltrán Breña ,
Marcelo F. Martinez ,
S.S. Perez-Garnelo ,
M.A. Ramirez ,
A. Gutiérrez-Adán  and
J. De la Fuente
A.T. Palasz*
Affiliation:
Dpto. de Reproducción Animal y Conservación de Recursos Zoogenéticos, INIA, Ctra de la Coruña Km 5.9, Madrid 28040, Spain.
P. Beltrán Breña
Affiliation:
Dpto. de Reproducción Animal y Conservación de Recursos Zoogenéticos, INIA, Ctra de la Coruña Km 5.9, Madrid 28040, Spain.
Marcelo F. Martinez
Affiliation:
Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK Canada S7N 5B4.
S.S. Perez-Garnelo
Affiliation:
Dpto. de Reproducción Animal y Conservación de Recursos Zoogenéticos, INIA, Ctra de la Coruña Km 5.9, Madrid 28040, Spain.
M.A. Ramirez
Affiliation:
Dpto. de Reproducción Animal y Conservación de Recursos Zoogenéticos, INIA, Ctra de la Coruña Km 5.9, Madrid 28040, Spain.
A. Gutiérrez-Adán
Affiliation:
Dpto. de Reproducción Animal y Conservación de Recursos Zoogenéticos, INIA, Ctra de la Coruña Km 5.9, Madrid 28040, Spain.
J. De la Fuente
Affiliation:
Dpto. de Reproducción Animal y Conservación de Recursos Zoogenéticos, INIA, Ctra de la Coruña Km 5.9, Madrid 28040, Spain.
*
1All correspondence to: A.T. Palasz. Ministerio De Educación y Ciencia, INIA, Ctra. de La Coruna km 5, 9 Madrid 28040, Spain. Tel: +34 91 347 40 23. Fax: +34 91 347 40 14. e-mail: [email protected]
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Summary

Hyaluronan (HA) is glycosaminoglycan that is present from the start of embryonic development and its role and concentration increases with embryo development. The objective of this study was to evaluate if the presence of HA in TCM-199 culture medium had an effect on the development and quality of bovine embryos. There was no effect of HA on the total number of zygotes developing to blastocysts on day 7, however more expanded and hatched blastocyst stages were observed on days 8 and 9 in the group supplemented with HA (p < 0.05). Following freeze/thawing, significantly more (p < 0.05) embryos cultured in medium supplemented with HA hatched than those cultured in TCM-199 alone or those with BSA. Medium supplemented with HA and BSA significantly increased the level of expression of glucose metabolism Glut-1 gene and embryo compaction Cx43 gene (p < 0.05), and had no effect on Glut-5 and IGF-II expression. In addition, HA presence in culture decreased the level of expression of apoptosis Bax and oxidative stress SOX genes (p < 0.05). There was significant difference in total number of nuclei between TCM-199 medium only and the remaining media containing BSA or HA plus BSA, between which there was no difference. In summary, our results indicate that the addition of high molecular weight HA to TCM-199 medium that contains BSA on day 4 of culture improved embryo development to hatching and hatched blastocysts and the quality of produced embryos, which were superior to embryos cultured without HA addition.

Keywords

Culture and developmentEmbryoFreezingGene expressionHyaluronanNuclei counts
Type
Research Article
Information
Zygote , Volume 16 , Issue 1 , February 2008 , pp. 39 - 47
Copyright
Copyright © Cambridge University Press 2008

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