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Development and quality of porcine embryos in different culture system and embryo-producing methods

Published online by Cambridge University Press:  01 February 2007

S-A. Ock ,
S-L. Lee ,
J-G. Kim ,
B-M. Kumar ,
S. Balasubramanian ,
S-Y. Choe  and
G-J. Rho
S-A. Ock
Affiliation:
Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Chinju, Republic of Korea660–701.
S-L. Lee
Affiliation:
Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Chinju, Republic of Korea660–701.
J-G. Kim
Affiliation:
Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Chinju, Republic of Korea660–701.
B-M. Kumar
Affiliation:
Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Chinju, Republic of Korea660–701.
S. Balasubramanian
Affiliation:
Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Chinju, Republic of Korea660–701. Department of Clinics, Madras Veterinary College, Tamilnadu Veterinary and Animal Sciences University, Chennai–600 007, Tamil Nadu, India.
S-Y. Choe
Affiliation:
Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Chinju, Republic of Korea660–701.
G-J. Rho*
Affiliation:
Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Chinju, Republic of Korea660–701.
*
All correspondence to: G-J. Rho, Department of Obstetrics and Theriogenology, College of Veterinary Medicine, Gyeongsang National University, 900 Gazwa, Chinju, Republic of Korea 660–701. Tel: +82 55 751 5824. Fax: +82 55 751 5803. e-mail: [email protected]
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Summary

In this study, the developmental ability and cellular composition of porcine IVF, parthenote and somatic cell nuclear transfer (SCNT) embryos were evaluated following different in vitro culture systems. Group 1, embryos were cultured in NCSU-23 with 5.55 mM D-glucose (NCSU+) until day 6 on 20% O2 or 5% O2 (Group 2). Group 3, embryos were cultured in D-glucose-free NCSU-23 (NCSU−) with 0.17 mM Na pyruvate/2.73 mM Na lactate for 58 h and subsequently cultured in NCSU+ until day 6 (NCSU −/+) on 20% O2 or 5% O2 (Group 4). IVF blastocysts did not differ significantly with O2 concentrations, but differed significantly with major energy source (glucose and pyruvate/lactate). In Group 3 and 4 IVF blastocysts, the total cell number and apoptosis rates were not significantly different with different O2 concentrations. Blastocyst rate, total cell number and apoptosis rate in Groups 3 and 4 parthenote embryos also were not significantly different. Parthenote and SCNT, under the same culture treatment, exhibited significant differences in blastocyst and apoptosis rates (47.5 ± 16.1 vs. 24.0 ± 4.0 and 4.9 ± 9.0 vs. 22.8 ± 23.3). Apoptosis-generating rate increased in the order parthenote, IVF and then SCNT. In conclusion, in vitro development of porcine embryos was not affected by O2 concentrations but was affected by major energy source. Even so, the concentration of each major energy source and the timing of its inclusion in culture could accomplish relatively high embryonic development, the apoptosis rate stressed that more work still needs to be done in developing a better defined culture system that could support SCNT embryos equivalent to in vivo preimplantation porcine embryos.

Keywords

ApoptosisDevelopmentEmbryo culture systemGas atmospherePorcine
Type
Research Article
Information
Zygote , Volume 15 , Issue 1 , February 2007 , pp. 1 - 8
Copyright
Copyright © Cambridge University Press 2007

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