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Detection of prothymosin alpha in oocytes and embryos of Bufo arenarum

Published online by Cambridge University Press:  01 November 1998

Gustavo A. Barisone ,
Félix V. Vega ,
Fernando Dominguez  and
Marcelo O. Cabada
Gustavo A. Barisone
Affiliation:
Area Biología y PROMUBIE, Facultad de Ciencias Bioquímicas y Farmaceuticas, Universidad Nacional de Rosario, Argentina
Félix V. Vega
Affiliation:
Departamento de Fisíologia, Facultad de Veterinaria, Universidad de Santiago de Compostela, Spain
Fernando Dominguez
Affiliation:
Departamento de Fisiología, Facultad de Medicina, Universidad de Santiago de Compostela, Spain
Marcelo O. Cabada
Affiliation:
Area Biología y PROMUBIE, Facultad de Ciencias Bioquímicas y Farmaceuticas, Universidad Nacional de Rosario, Argentina
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Abstract

Prothymosin alpha (PTA) was detected by immunocytological and biochemical methods in oocytes at different stages of oogenesis, and in early embryos of the amphibian Bufo arenarum. In all cases PTA was detected in the nucleus and was absent from the cytoplasm. This indicates that this protein could act at the level of regulating transcription. Western blots were carried out using polyclonal antibodies with extracts of embryos at different stages of development from early fertilisation up to neural tube. With this method PTA was detected in all the samples under study.

Keywords

Bufo arenarumEmbryosNucleusOocytesProthymosin alpha
Type
Research Article
Information
Zygote , Volume 6 , Issue 4 , November 1998 , pp. 347 - 350
Copyright
1998 Cambridge University Press

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