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Cytogenetic studies of oocyte fusion products

Published online by Cambridge University Press:  26 September 2008

Carme Nogués ,
Montserrat Ponsà ,
Josep Egozcue  and
Francesca Vidal
Carme Nogués
Affiliation:
Department de Biologia Cellular i Fisiologia, Universitat Autònoma de Barcelona, Barcelona, Spain.
Montserrat Ponsà
Affiliation:
Department de Biologia Cellular i Fisiologia, Universitat Autònoma de Barcelona, Barcelona, Spain.
Josep Egozcue
Affiliation:
Department de Biologia Cellular i Fisiologia, Universitat Autònoma de Barcelona, Barcelona, Spain.
Francesca Vidal*
Affiliation:
Department de Biologia Cellular i Fisiologia, Universitat Autònoma de Barcelona, Barcelona, Spain.
*
Dra Francesca Vidal, Unitat de Biologia Cellular, Facultat de Ciències, Universitat Autònoma de Barcelona, S-08193 Bellaterra (Barcelona), Spain. Telephone: 34-3-581.27.81. Fax: 34-3-581.22.95. email: [email protected].
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Summary

We describe for the first time the cytogenetic characteristics of mouse ‘embryos’ obtained by oocyte fusion (oocyte fusion products; OFP). Our results indicate that, after fusion, meiosis II is resumed correctly, with extrusion of two haploid polar bodies, and that metaphase synchronisation of the two haploid sets and chromosome segregation during the first cleavage are also normal.

Keywords

CytogeneticsEmbryosMouseOocyte fusion products
Type
Article
Information
Zygote , Volume 3 , Issue 1 , February 1995 , pp. 27 - 29
Copyright
Copyright © Cambridge University Press 1995

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References

Barton, S.C., Surani, M.A.H. & Norris, M.L. (1984). Role of paternal and maternal genomes in mouse development. Nature 311, 374–6.CrossRefGoogle ScholarPubMed
Gulyas, B.J. (1986). Oocyte fusion. In Developmental Biology: A Comprehensive Synthesis. Manipulation of Mammalian Development, ed. Gwatkin, R.B.L., pp. 57–80. New York: Plenum Publishing.CrossRefGoogle Scholar
Gulyas, B.J., Wood, M. & Whittingham, D.G. (1984). Fusion of oocytes and development of oocyte fusion products in the mouse. Dev. Biol. 101, 246–50.CrossRefGoogle ScholarPubMed
Kaufman, M.H.. (1983). Mammalian parthenogenesis: background to experimental studies, terminology and path ways of development. In Early Mammalian Development: Parthenogenetic Studies, ed. Barlow, P.W., Green, P.B. & Wylie, C.C., pp. 1–9. Cambridge: Cambridge University Press.Google Scholar
McGrath, J. & Solter, D. (1984). Completion of mouse embryogenesis requires both the maternal and paternal genomes. Cell 37, 179–83.CrossRefGoogle ScholarPubMed
Mintz, B. (1962). Experimental study of the developing mammalian egg: removal of the zona pellucida. Science 138, 594–7.CrossRefGoogle ScholarPubMed
Nogués, C., Ponsà, M., Egozcue, J. & Vidal, F.. (1994). Ultrastructural studies of early mouse embroys obtained by oocyte fusion. Zygote 2, 15–28.CrossRefGoogle Scholar
Quinn, P., Barros, C. & Whittingham, D.G. (1982). Preservation of hamster oocytes to assay the fertilizing capacity of human spermatozoa. J. Reprod. Fert. 66, 161–8.CrossRefGoogle ScholarPubMed
Santaló, J., Estop, A.M. & Egozcue, J. (1986). The chromosome complement of first-cleavage mouse embryos after in vitro fertilization. J. In Vitro Fert. Embryo Transf. 3, 99–105.CrossRefGoogle ScholarPubMed
Soupart, P. (1982). Initiation of mouse embryonic development by oocyte fusion. In In virto Fertilization and Embryo Transfer, ed Hafez, E.S.E. & Senm, K., pp. 51–63. Lancaster: MTP Press.Google Scholar
Surani, M.A.H. (1991). Influence of genome imprinting on gene expresion, phenotypic variations and developement. Hum. Reprod. 6, 45–51.CrossRefGoogle Scholar
Surani, M.A.H., Barton, S.C. & Norris, M.L. (1986). Nuclear transplantation in the mouse: heritable differences between parental genomes after activation of the embryonic genome. Cell 45, 127–36.CrossRefGoogle ScholarPubMed
Tarkowski, A.K. (1966). An air-drying method for chromosome preparations from mouse eggs. Cytogentics 5, 394–400.CrossRefGoogle Scholar
Whittingham, D.G. (1971). Culture of mouse ova. J. Reprod. Fert., Suppl. 14, 7–21.Google ScholarPubMed