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Cortical granule exocytosis is triggered by different thresholds of calcium during fertilisation in sea urchin eggs

Published online by Cambridge University Press:  15 January 2010

John C. Matese  and
David R. McClay
John C. Matese
Affiliation:
Zoology Department, Duke University, Durham, North Carolina, USA
David R. McClay*
Affiliation:
Zoology Department, Duke University, Durham, North Carolina, USA
*
D.R. McClay, Developmental, Cell and Molecular Biology Program, Zoology Department, Box 91000, Levine Science Research Building, Duke University, Durham, North Carolina 27708-1000, USA. Telephone: +1 (919) 613-8188. Fax: +1 (919) 613-8177. e-mail: [email protected].
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Summary

In sea urchin eggs, fertilisation is followed by a calcium wave, cortical granule exocytosis and fertilisation envelope elevation. Both the calcium wave and cortical granule exocytosis sweep across the egg in a wave initiated at the point of sperm entry. Using differential interference contrast (DIC) microscopy combined with laser scanning confocal microscopy, populations of cortical granules undergoing calcium-induced exocytosis were observed in living urchin eggs. Calcium imaging using the indicator Calcium Green-dextran was combined with an image subtraction technique for visual isolation of individual exocytotic events. Relative fluorescence levels of the calcium indicator during the fertilisation wave were compared with cortical fusion events. In localised regions of the egg, there is a 6s delay between the detection of calcium release and fusion of cortical granules. The rate of calcium accumulation was altered experimentally to ask whether this delay was necessary to achieve a threshold concentration of calcium to trigger fusion, or was a time-dependent activation of the cortical granule fusion apparatus after the ‘triggering’ event. Calcium release rate was attenuated by blocking inositol 1,4,5-triphospate (InsP3)-gated channels with heparin. Heparin extended the time necessary to achieve a minimum concentration of calcium at the sites of cortical granule exocytosis. The data are consistent with the conclusion that much of the delay observed normally is necessary to reach threshold concentration of calcium. Cortical granules then fuse with the plasma membrane. Further, once the minimum threshold calcium concentration is reached, cortical granule fusion with the plasma membrane occurs in a pattern suggesting that cortical granules are non-uniform in their calcium sensitivity threshold.

Keywords

CalciumCortical granulesExocytosisFertilisation reactionUrchin
Type
Research Article
Information
Zygote , Volume 6 , Issue 1 , February 1998 , pp. 55 - 63
Copyright
Copyright © Cambridge University Press 1998

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