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Connexin37 mRNA expression in in vivo and in vitro mouse oocyte

Published online by Cambridge University Press:  01 May 2009

Bao Ying Yin ,
Yong Zhang ,
Jian Hong Sun ,
Ji Xia Li  and
Ye Fei Ma
Bao Ying Yin
Affiliation:
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province, People's Republic of China.
Yong Zhang*
Affiliation:
College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi Province 712100, People's Republic of China.
Jian Hong Sun
Affiliation:
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province, People's Republic of China.
Ji Xia Li
Affiliation:
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province, People's Republic of China.
Ye Fei Ma
Affiliation:
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province, People's Republic of China.
*
All correspondence to Yong Zhang. College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi Province 712100, People's Republic of China. Tel: +86 29 87080085. Fax: +86 29 87080085. e-mail: [email protected]
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Summary

To evaluate gene expression of Connexin37 (Cx37) in oocytes from in vitro follicles at different stages, mouse preantral follicles were isolated and cultured for 12 days in vitro. Compared with in vitro follicles, follicles grown in vivo were collected at day 14 (d14), d16, d18, d20, d22 and d24 with the same stages for gene expression of Cx37 in oocytes. Our results showed that Cx37 mRNA increased along with follicular development, reached the highest level at the onset of antrum cavity formation and decreased after antrum formation in both in vivo and in vitro mouse oocytes. However, Cx37 mRNA was significant higher (p < 0.01) in in vitro cultured oocytes than in vivo oocytes. Moreover, significantly higher levels of Cx37 mRNA were found in oocytes from in vitro disrupted follicles (p < 0.01) and non-grown follicles (p < 0.05) than those from normal follicles with a similar size. These data determine temporal gene expression of Cx37 in oocytes from follicules at different stages and indicate that the gene expression level of Cx37 in oocytes could be evaluated as a criterion to the regulatory mechanism of Cx37 in an in vitro model.

Keywords

ConnexinFollicle cultureMouseOocyte development
Type
Research Article
Information
Zygote , Volume 17 , Issue 2 , May 2009 , pp. 163 - 168
Copyright
Copyright © Cambridge University Press 2009

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