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Comparison of sperm preparation methods to improve the recovery of mature spermatozoa in sub-fertile males

Published online by Cambridge University Press:  08 July 2022

Chiara Fasano ,
Giuseppe D’Andolfi ,
Loredana Di Matteo ,
Claudia Forte ,
Brian Dale Open the ORCID record for Brian Dale [Opens in a new window]  and
Elisabetta Tosti Open the ORCID record for Elisabetta Tosti [Opens in a new window]
Chiara Fasano
Affiliation:
Centro Fecondazione Assistita (CFA), Via Tasso 480, 80123 Naples, Italy
Giuseppe D’Andolfi
Affiliation:
Centro Fecondazione Assistita (CFA), Via Tasso 480, 80123 Naples, Italy
Loredana Di Matteo
Affiliation:
Centro Fecondazione Assistita (CFA), Via Tasso 480, 80123 Naples, Italy
Claudia Forte
Affiliation:
Centro Fecondazione Assistita (CFA), Via Tasso 480, 80123 Naples, Italy
Brian Dale
Affiliation:
Centro Fecondazione Assistita (CFA), Via Tasso 480, 80123 Naples, Italy
Elisabetta Tosti*
Affiliation:
Centro Fecondazione Assistita (CFA), Via Tasso 480, 80123 Naples, Italy
*
Author for correspondence: Elisabetta Tosti. Centro Fecondazione Assistita (CFA), Via Tasso 480, 80123 Naples, Italy. Tel: +39 081641689. E-mail: [email protected]
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Summary

The integrity of chromatin in the spermatozoon is essential for reproductive outcome. The aim of this study was to evaluate the most effective and cost-effective method to reduce the percentage of spermatozoa with defects in chromatin decondensation for use in assisted reproductive technologies (ART) procedures. Sperm samples from 15 sub-fertile males were examined at CFA Naples to determine the sperm decondensation index (SDI), using the aniline blue test, before and after preparation, comparing density gradients with two different swim-up approaches. All three techniques led to a reduction in decondensed spermatozoa with no statistical difference (P > 0.05) between the control and the treated sperm. In contrast, we found a highly significant decrease in SDI (P < 0.01) after the two swim-up methods in all the samples, confirming the efficacy of these methods in lowering the percentage of chromatin compaction damage. There was no statistical difference between the two swim-up methods, however swim-up from the pellet led to improved count, motility and the percentage of normal condensed spermatozoa. We suggest that swim-up from the pellet be used in ART on sub-fertile males, both to reduce cell stress by multiple centrifugation and improve the recovery rate of mature spermatozoa.

Keywords

Chromatin condensationGradientSperm preparationSpermatozoaSwim-up
Type
Research Article
Information
Zygote , Volume 30 , Issue 5 , October 2022 , pp. 664 - 673
Copyright
© The Author(s), 2022. Published by Cambridge University Press

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